Overview

This detailed volume explores techniques to study reactive oxygen species (ROS) in plants and to characterize their roles in development and stress responses. Beginning with a section on strategies to induce ROS production, the book continues with methods to visualize ROS and detect changes in redox homeostasis, small-scale and targeted analyses for investigating the effects of ROS accumulation during stress on plant physiology and metabolism, as well as systems biology approaches to understand ROS functions. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and practical, Reactive Oxygen Species in Plants: Methods and Protocols serves as a vital resource that any researcher, and in particular young researchers, can use and adapt to further our knowledge of this dynamic area of plant science.

Full Product Details

Author:   Amna Mhamdi
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2022
Volume:   2526
Weight:   0.787kg
ISBN:  

9781071624685

ISBN 10:   1071624687
Pages:   298
Publication Date:   04 June 2022
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Part I: Strategies to Induce ROS Production   1. Modification of Chloroplast Antioxidant Capacity by Plastid Transformation             Shengchun Li, Pan Shen, Bipeng Wang, Xiujie Mu, Mimi Tian, Tao Chen, and Yi Han   2. Analysis of Ascorbate Metabolism in Arabidopsis under High-Light-Stress             Takanori Maruta and Takahiro Ishikawa   3. Genetic Manipulation of Reactive Oxygen Species (ROS) Homeostasis Utilizing CRISPR/Cas9-Based Gene Editing in Rice             Sheng Xu, Tao Chen, Mimi Tian, Marie-Sylviane Rahantaniaina, Linlin Zhang, Ren Wang, Wei Xuan, and Yi Han   4. Studying Plant Stress Reactions In Vivo by PAM Chlorophyll Fluorescence Imaging             Alexey Shapiguzov and Jaakko Kangasjärvi   Part II: Methods to Visualize ROS and to Detect Changes in Redox Homeostasis   5. Live Monitoring of ROS-Induced Cytosolic Redox Changes with roGFP2-Based Sensors in Plants             José Manuel Ugalde, Lara Fecker, Markus Schwarzländer, Stefanie J. Müller-Schüssele, and Andreas J. Meyer   6. Quantitative Measurement of Ascorbate and Glutathione by Spectrophotometry             Graham Noctor and Amna Mhamdi   7. Measurement of NAD(P)H and NADPH-Generating Enzymes             Amna Mhamdi, Frank Van Breusegem, and Graham Noctor   8. Quantitative Analysis for ROS-Producing Activity and Regulation of Plant NADPH Oxidases in HEK293T Cells             Sachie Kimura, Hidetaka Kaya, Kenji Hashimoto, Michael Wrzaczek, and Kazuyuki Kuchitsu   Part III: Small-Scale Targeted Analysis of ROS Accumulation during Stress and Effects on Plant Physiology   9. Estimation of the Level of Abasic Sites in Plant mRNA Using Aldehyde Reactive Probe             Jagna Chmielowska-Bąk, Karolina Izbiańska-Jankowska, and Joanna Deckert   10. A Simplified Method to Assay Protein Carbonylation by Spectrophotometry             Corentin Moreau and Emmanuelle Issakidis-Bourguet   11. In Vitro Biochemical Analysis of Recombinant Plant Proteins under Oxidation             Zeya Chen and Jingjing Huang   12. Methods to Analyze the Redox Reactivity of Plant Proteins             Thualfeqar Al-Mohanna, George V. Popescu, and Sorina C. Popescu   13. Determination of ROS-Induced Lipid Peroxidation by HPLC-Based Quantification of Hydroxy Polyunsaturated Fatty Acids             Brigitte Ksas and Michel Havaux   14. Detection of Lipid Peroxidation-Derived Free Azelaic Acid, a Biotic Stress Marker and Other Dicarboxylic Acids in Tobacco by Reversed Phase HPLC-MS under Non-Derivatized Conditions             Attila L. Ádám, György Kátay, András Künstler, and Lóránt Király   15. Determination of Reactive Carbonyl Species, which Mediate Reactive Oxygen Species Signals in Plant Cells             Jun'ichi Mano, Md. Sanaullah Biswas, Koichi Sugimoto, and Yoshiyuki Murata   16. Measuring Stress-Induced Changes in Defense Phytohormones and Related Compounds             Caroline Lelarge-Trouverie, Amna Mhamdi, Florence Guérard, and Graham Noctor   Part IV: Systems Biology Approaches to Understand ROS Functions   17. Targeted Mass Spectrometry Analysis of Protein Phosphorylation by Selected Ion Monitoring Coupled to Parallel Reaction Monitoring (tSIM/PRM)             Jesús Pascual and Saijaliisa Kangasjärvi   18. Quantitative Analysis of Post-Translational Modifications of Plant Histones             Hana Kuchaříková, Zuzana Plšková, Zbyněk Zdráhal, Miloslava Fojtová, Pavel Kerchev, and Gabriela Lochmanová   19. Characterization of RBPome in Oxidative Stress Conditions             Zhicheng Zhang, Evy Timmerman, Francis Impens, and Frank Van Breusegem   20. Analysis of ROS-Triggered Changes in the Transcriptome             Patrick Willems

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