Overview

This volume compiles new experimental approaches and concepts focusing mostly, but not solely, on ways to manipulate and regulate Ras activity and its downstream signaling output. Chapters detail standard methodologies, biochemical methods, Ras processing trafficking and localization, Ras signaling and inhibition, and in vivo models for studying Ras function. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls.   Authoritative and accessible, Ras Activity and Signaling: Methods and Protocols aims to provide support and guidance to lab workers in their work on Ras GTPases and in the design of new projects requiring novel methodologies.

Full Product Details

Author:   Ignacio Rubio ,  Ian Prior
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2021
Volume:   2262
Weight:   0.854kg
ISBN:  

9781071611920

ISBN 10:   1071611925
Pages:   439
Publication Date:   12 May 2022
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Part I: Generic Ras Biology 1. Ras Variant Biology and Contributions to Human Disease Ian A. Prior   2. Regulation of the Small GTPase Ras and its Relevance to Human Disease Kayla R. Kulhanek, Jeroen P. Roose, and Ignacio Rubio   Part II: Methods in Ras Biochemistry 3. Precise Characterization of KRAS4B Proteoforms by Combining Immunoprecipitation with Top-Down Mass Spectrometry Lauren M. Adams, Caroline J. DeHart, and Neil L. Kelleher   4. Absolute Quantitation of GTPase Protein Abundance Fiona E. Hood, Yasmina M. Sahraoui, Rosalind E. Jenkins, and Ian A. Prior   5. Validation of Isoform- and Mutation-specific RAS Antibodies Andrew M. Waters and Channing J. Der   6. Production and Membrane Binding of N-terminally Acetylated, C-terminally Farnesylated and Carboxymethylated KRAS4b Simon Messing, Constance Agamasu, Matt Drew, Caroline DeHart, Andrew G. Stephen,  and William K. Gillette   7. Active GTPase Pulldown Protocol Martin J. Baker and Ignacio Rubio   8. Methods to Monitor Ras Activation State Kari Kopra and Harri Härmä   9. NMR Detection Methods for Profiling RAS Nucleotide Cycling Ryan C. Killoran and Matthew J. Smith   Part III: Methods in Ras Processing, Trafficking, and Localization 10. Ras Diffusion and Interactions with the Plasma Membrane Measured by FRAP Variations Orit Gutman, Marcelo Ehrlich, and Yoav I. Henis   11. Understanding Ras Spatial Cycles through Reaction-Diffusion Simulations Malte Schmick and Philippe I. H. Bastiaens   12. Super-Resolution Imaging and Spatial Analysis of RAS on Intact Plasma Membrane Sheets Yong Zhou and John F. Hancock   13. FLIM-FRET Analysis of Ras Nanoclustering and Membrane-Anchorage Hanna Parkkola, Farid Ahmad Siddiqui, Christina Oetken-Lindholm, and Daniel Abankwa   14. Assessment of Plasma Membrane Fatty Acid Composition and Fluidity Using Imaging Flow Cytometry Natividad R. Fuentes, Michael L. Salinas, Xiaoli Wang, Yang-Yi Fan, and Robert S. Chapkin   15. Spatiotemporal Imaging of Small GTPase Activity using Conformational Sensors for GTPase Activity (COSGA) Yao-Wen Wu   Part IV: Methods in Ras Signaling and Inhibition 16. Using BioID to Characterize the RAS Interactome Hema Adhikari and Christopher M. Counter   17. Probing RAS function with Monobodies Imran Khan and John P. O’Bryan   18. Detection of Endogenous RASSF1A Interacting Proteins Howard Donninger, Desmond Harrell-Stewart, and Geoffrey J. Clark   19. Mathematical Modeling to Study KRAS Mutant-Specific Responses to Pathway Inhibition Edward C. Stites   20. A Facile Method to Engineer Mutant Kras Alleles in an Isogenic Cell Background Konstantin Budagyan and Jonathan Chernoff   21. RASless MEFs as a Tool to Study RAS-dependent and -independent Functions Carmen G. Lechuga, Marina Salmón, Guillem Paniagua, Carmen Guerra, Mariano Barbacid, Matthias Drosten   Part V: Methods in vivo Ras Biology 22. Generation of Patient-derived Colorectal Cancer Organoids for RAS Studies Dirk Schumacher, Joseph L. Regan, Dorothea Przybilla, and Reinhold Schäfer   23. Ras-GEF Mouse Models for the Analysis of Ras Biology and Signaling Alberto Fernández-Medarde and Eugenio Santos   24. Studying Metabolic Abnormalities in the Costello Syndrome HRAS G12V Mouse Model: Isolation of Mouse Embryonic Fibroblasts and Their In Vitro Adipocyte Differentiation Miray Fidan, Saravanakkumar Chennappan, and Ion Cristian Cirstea   25. Dissecting Oncogenic RAS Signalling in Melanoma Development in Genetically Engineered Zebrafish Models Andrew P. Badrock and Adam Hurlstone   26. Ras, Ral, and Rap1 in C. elegans Neal R. Rasmussen and David J. Reiner

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