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OverviewRadiation Oncology and Radiotherapy, Part A, Volume 172 in the Methods in Cell Biology series, highlights advances in the field, with this new volume presenting interesting chapters on timely topics, including DNA damage quantification by the COMET assay, Immunofluorescence microscopy-assisted quantification of ATM and ATR activation in irradiated cells, Immunoblotting-based characterization of the DNA damage response, Assessment of lipid peroxidation in irradiated cells, A simple method to assess clonogenic survival of irradiated cancer cells, Quantification of beta-galactosidase activity as a marker of radiation-driven cellular senescence, Cytofluorometric assessment of cell cycle progression in irradiated cells, and more. Other sections cover Assessment of transcription inhibition as a characteristic of immunogenic cell death, Assessment of eIF2a phosphorylation during immunogenic cell death, Quantification of cytosolic DNA species by immunofluorescence and automated image analysis, Flow cytometry-assisted quantification of CALR exposure during immunogenic cell death, Interference of immunogenic anticancer therapy by artificially controlled calreticulin secretion from tumor cells, along with many additional topics of interest. Full Product DetailsAuthor: Ai Sato (Stich Radiation Oncology, New York, NY, USA) , Jeffrey Kraynak (research laboratory specialist at Stich Radiation Oncology, New York, NY, USA.) , Ariel E. Marciscano (Assistant Professor of Radiation Oncology, Weill Cornell Medical College) , Lorenzo Galluzzi (Assistant Professor of Cell Biology in Radiation Oncology, Department of Radiation Oncology, Weill Cornell Medical College, NY, USA)Publisher: Elsevier Science & Technology Imprint: Academic Press Inc Weight: 0.610kg ISBN: 9780323899499ISBN 10: 0323899498 Pages: 214 Publication Date: 01 September 2022 Audience: Professional and scholarly , Professional & Vocational Format: Hardback Publisher's Status: Active Availability: Manufactured on demand  We will order this item for you from a manufactured on demand supplier. Table of Contents1. DNA damage quantification by the COMET assay Mathias Weyland 2. Immunofluorescence microscopy-assisted quantification of ATM and ATR activation in irradiated cells Ilio Vitale 3. Immunoblotting-based characterization of the DNA damage response Ilio Vitale 4. Assessment of lipid peroxidation in irradiated cells Boyi Gan, Chao Mao, Amber Horbath and Guang Lei 5. A simple method to assess clonogenic survival of irradiated cancer cells Maria Esperanza Rodriguez Ruiz 6. Quantification of beta-galactosidase activity as a marker of radiation-driven cellular senescence Giulia Petroni 7. Cytofluorometric assessment of cell cycle progression in irradiated cells Giulia Petroni, Ai Sato, Aitziber Buqué Martínez and Lorenzo Galluzzi 8. Assessment of transcription inhibition as a characteristic of immunogenic cell death Guido Kroemer, Oliver Kepp, Giulia Cerrato and Allan Sauvat 9. Assessment of eIF2a phosphorylation during immunogenic cell death Guido Kroemer, Oliver Kepp and Lucillia Bezu 10. Quantification of cytosolic DNA species by immunofluorescence and automated image analysis Lorenzo Galluzzi 11. Flow cytometry-assisted quantification of CALR exposure during immunogenic cell death Jitka Fucikova 12. Interference of immunogenic anticancer therapy by artificially controlled calreticulin secretion from tumor cells Guido Kroemer, Oliver Kepp, Peng LIU and Liwei Zhao 13. Assessment of type I interferon responses as a feature of immunogenic cell death. Guido Kroemer, Oliver Kepp, Sabrina Forveille, Allan Sauvat and Liwei Zhao 14. Assessment of immunological memory formation in vivo Guido Kroemer, Oliver Kepp, Liwei Zhao and Peng LIU 15. Characterization of the SASP of cancer cells experiencing DNA damage Francis Rodier 16. ELISA-based quantification of type I IFN secretion by irradiated cancer cells Claire I. Vanpouille-Box 17. RT-PCR-assisted quantification of type I IFN responses by irradiated cancer cells Lorenzo Galluzzi 18. Methods to characterize the exosomal output of irradiated cancer cells Sheila Spada 19. Cytofluorometric assessment of acute cell death responses driven by radiation therapy Lorenzo GalluzziReviewsAuthor InformationAi Sato is a research specialist at Stich Radiation Oncology, New York, NY, USA Jeffrey Kraynak is a research laboratory specialist at Stich Radiation Oncology, New York, NY, USA. Dr. Ariel E. Marciscano is an assistant professor of Radiation Oncology at Weill Cornell Medical College. Lorenzo Galluzzi is Assistant Professor of Cell Biology in Radiation Oncology at the Department of Radiation Oncology of the Weill Cornell Medical College, Honorary Assistant Professor Adjunct with the Department of Dermatology of the Yale School of Medicine, Honorary Associate Professor with the Faculty of Medicine of the University of Paris, and Faculty Member with the Graduate School of Biomedical Sciences and Biotechnology of the University of Ferrara, the Graduate School of Pharmacological Sciences of the University of Padova, and the Graduate School of Network Oncology and Precision Medicine of the University of Rome “La Sapienza. Moreover, he is Associate Director of the European Academy for Tumor Immunology and Founding Member of the European Research Institute for Integrated Cellular Pathology. Galluzzi is best known for major experimental and conceptual contributions to the fields of cell death, autophagy, tumor metabolism and tumor immunology. He has published over 450 articles in international peer-reviewed journals and is the Editor-in-Chief of four journals: OncoImmunology (which he co-founded in 2011), International Review of Cell and Molecular Biology, Methods in Cell biology, and Molecular and Cellular Oncology (which he co-founded in 2013). Additionally, he serves as Founding Editor for Microbial Cell and Cell Stress, and Associate Editor for Cell Death and Disease, Pharmacological Research and iScience. Tab Content 6Author Website:Countries AvailableAll regions |
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