Quorum Sensing: Methods and Protocols

Author:   Livia Leoni ,  Giordano Rampioni
Publisher:   Humana Press Inc.
Edition:   1st ed. 2018
Volume:   1673
ISBN:  

9781493973088


Pages:   375
Publication Date:   15 November 2017
Format:   Hardback
Availability:   Manufactured on demand   Availability explained
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Quorum Sensing: Methods and Protocols


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Author:   Livia Leoni ,  Giordano Rampioni
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   1st ed. 2018
Volume:   1673
Weight:   8.782kg
ISBN:  

9781493973088


ISBN 10:   1493973088
Pages:   375
Publication Date:   15 November 2017
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand   Availability explained
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Use of Whole Cell Bioassays for Screening Quorum Signaling, Quorum-Interference, and Biofilm Dispersion.- Detection of 2-Alkyl-4-Quinolones Using Biosensors.- “Hot Stuff”: The Many Uses of a Radiolabel Assay in Detecting Acyl-Homoserine Lactone Quorum Sensing Signals.- Liquid Chromatography/Mass Spectrometry (LC/MS) for the Detection and Quantification of N-Acyl-L-Homoserine Lactones (AHLs) and 4-Hydroxy-2-Alkylquinolines (HAQs).- Detection of the Bacterial Quorum Sensing Signaling Molecules N-Acyl Homoserine Lactones (HSL) and N-Acyl-Homoserine (HS) with an Enzyme-Linked Immunosorbent Assay (ELISA) and via Ultrahigh Performance Liquid Chromatography Coupled to Mass Spectrometry (UHPLC-MS).- Biosensors for the Detection and Quantification of AI-2 Class Quorum Sensing Compounds.- Detection of Agr-Type Autoinducing Peptides Produced by Staphylococcus aureus.- Ultra Performance Liquid Chromatography/Mass Spectrometryfor the Detection and Quantification of Diffusible Signal Factor (DSF)-Family Quorum Sensing Signals.- Rapid Electrochemical Detection of Pseudomonas aeruginosa Signaling Molecules by Boron-Doped Diamond Electrode.- Detection and Quantification of Butyrolactones from Streptomyces.- Fluorescence Quenching Studies of γ-Butyrolactone Binding Protein (CprB) from Streptomyces coelicolor A3(2).- Methods to Study Solo/Orphan Quorum Sensing Receptors.- Enzymatic Assays to Investigate Acyl-Homoserine Lactone Autoinducer Synthases.- Global Expression Analysis of Quorum Sensing-Controlled Genes by RNAseq.- Identification of AHL- and BDSF-Controlled Proteins in Burkholderia cenocepacia by Proteomics.- Imaging N-Acyl Homoserine Lactone Quorum Sensing In Vivo.- Assessing Pseudomonas aeruginosa Autoinducer Effects on Mammalian Epithelial Cells.- Animal Models for Pseudomonas aeruginosa Quorum Sensing Studies.- Methods to Study Quorum Sensing-Dependent Virulence and Movement of Phytopathogens In Planta.- Differential Equations Models to Study Quorum Sensing.- Qualitative and Quantitative Determination of Quorum Sensing Inhibition In Vitro.- A Co-Culture-Based Approach for Screening Campaigns Aimed at Identifying Novel Pseudomonas aeruginosa Quorum Sensing Inhibitors.- A Culture-Dependent Method for the Identification of Quorum Quenching Enzymes of Microbial Origin.- Directed Evolution of Quorum Quenching Enzymes: A Method for the Construction of a Directed Evolution Platform and Characterization of a Quorum-Quenching Lactonase from Geobacillus kaustophilus.- Generation of High-Sensitivity Monoclonal Antibodies Specific for Homoserine Lactones.- Identification of AI-2 Quorum Sensing Inhibitors in Vibrio harveyi through Structure-Based Virtual Screening.- Identification of Staphylococcal Quorum Sensing Inhibitors byQuantification of õ-Hemolysin with High Performance Liquid Chromatography.

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