Overview

This thorough book explores some of the most important methods and concepts affecting the quantitative analysis of the transport, targeting, and disposition of chemicals within cells, which in turn impact the macroscopic pharmacokinetics of chemical agents in the whole organism. The first half of the volume focuses on small organic molecules with drug-like characteristics, while the second half delves into the cellular pharmacokinetics of biologics and other macromolecules, including peptide therapeutics, cyclotides, antibodies, as well as nanoparticles, thus creating a comprehensive treatise that approaches cellular pharmacokinetics from the different perspectives of pharmaceutical scientists, chemical biologists, medicinal chemists, and protein engineers dealing with very different chemical agents spanning a wide range of sizes, physicochemical properties, and targeting mechanisms. Written for the Methods in Pharmacology and Toxicology series, chapters provide the kind of key detail and expert implementation advice that leads to excellent results in the lab. Synthetic biologists, biophysicists, and bioengineers are amongst the long list of scientists who could benefit from reading this book or from using it as a textbook.  Authoritative and practical, Quantitative Analysis of Cellular Drug Transport, Disposition, and Delivery builds on a long history of drug development and the adding of quantitative methods at the cellular scale in order to inspire new approaches to drug development that are better able to take advantage of phenomena such as soluble-to-insoluble phase transitions or bispecific targeting, which could ultimately be exploited for the development of more effective drug delivery systems and therapeutic agents.

Full Product Details

Author:   Gus R. Rosania ,  Greg M. Thurber
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2021
Weight:   0.891kg
ISBN:  

9781071612521

ISBN 10:   1071612522
Pages:   453
Publication Date:   08 June 2021
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Part I: Small Molecules   1. Measurement of Transcellular Transport Rates and Intracellular Drug Sequestration in the Presence of an Extracellular Concentration Gradient             Kyoung Ah Min and Gus R. Rosania   2. Kinetic Design for Establishing Long Term Stationary Cytosol Concentrations during Drug Transport across P-gp Expressing Confluent Cell Monolayers to Facilitate Measuring Cytosol Concentration, Fitting Drug Molar Partition Coefficients into the Cytosolic Monolayer of the Plasma Membrane and Kinetically Identifying Drug Uptake Transporters             Joe Bentz   3. In Vitro Methodologies to Assess Potential for Transporter-Mediated Drug-Drug Interactions             Jibin Li, Qing Wang, and Ismael J. Hidalgo   4. Determination of Fraction Unbound and Unbound Partition Coefficient to Estimate Intracellular Free Drug Concentration             Sangwoo Ryu, Keith Riccardi, Samantha Jordan, Nathaniel Johnson, and Li Di   5. Quantitative Analysis of Intracellular Drug Concentrations in Hepatocytes             Chitra Saran, James J. Beaudoin, Nathan D. Pfeifer, and Kim L.R. Brouwer   6. Quantification of Intracellular Drug Aggregates and Precipitates             Phillip Rzeczycki and Gus R. Rosania   7. Quantitative Phenotypic Analysis of Drug Sequestering Macrophage Subpopulations             Mikhail D. Murashov   8. Using an Integrated QSAR Model to Check Whether Small-Molecule Xenobiotics Will Accumulate in Biomembranes, with Particular Reference to Fluorescent Imaging Probes             Richard W. Horobin and Juan C. Stockert   9. Diversity-Oriented Fluorescence Library Approach (DOFLA) for Discovery of Cell-Permeable Probes for Applications in Live Cell Imaging             Dongdong Su and Young-Tae Chang   Part II: Macromolecules, Biologics, and Nanoparticles   10. Overcoming Cellular and Systemic Barriers to Design the Next Wave of Peptide Therapeutics             Jerome Hochman, Tomi Sawyer, and Ruchia Duggal   11. Intracellular Targeting of Cyclotides for Therapeutic Applications             Nicole Lawrence and David J. Craik   12. Cellular Trafficking of Monoclonal and Bispecific Antibodies             John J. Rhoden and Christopher M. Wiethoff   13. Quantitative Drug Target Imaging Using Paired-Agent Principles             Kenneth M. Tichauer, Negar Sadeghipour, Yu “Winston” Wang, Summer L. Gibbs, Jonathan T.C. Liu, and Kimberley S. Samkoe   14. Quantitative Determination of Intracellular Bond Cleavage             Joshua A. Walker, Michelle R. Sorkin, and Christopher A. Alabi   15. Development and Application of a Single Cell-Level PK-PD Model for ADCs             Shufang Liu and Dhaval K. Shah   16. Contribution of Non-Target Cells to the Disposition, Antitumor Activity, and Antigen-Independent Toxicity of Antibody Drug-Conjugates             David W. Meyer, Fu Li, and Robert P. Lyon   17. Tracking siRNA-Nanocarrier Assembly and Disassembly Using FRET             Lorenz Isert, Aditi Mehta, Friederike Adams, and Olivia M. Merkel   18. Sub-Cellular Drug Depots as Reservoirs for Small Molecule Drugs             Ran Li, Ralph Weissleder, and Miles A. Miller

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