Pulsed Field Gel Electrophoresis: A Practical Approach
Author:   A. P. Monaco (Senior Scientist, ICRF, Institute of Molecular Medicine, John Radcliffe Hospital, Oxford)
Publisher:   Oxford University Press
Volume:   158
ISBN:  

9780199635351


Pages:   216
Publication Date:   12 October 1995
Format:   Paperback
Availability:   In Print   Availability explained
Limited stock is available. It will be ordered for you and shipped pending supplier's limited stock.

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Pulsed Field Gel Electrophoresis: A Practical Approach


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Overview

PFGE is the most reliable method for separating large fragments of DNA and has had a major impact on biological research. This book presents detailed chapters on both the theory behind PFGE amd protocols for most of its major research and diagnostic applications. This book is intended for researchers at all levels in labs involved in molecular human genetics, mammalian genetics (mouse, rat, etc), molecular microbiologists, molecular parasitologists, biochemists who use molecular biology techniques, and some molecular diagnostic labs.

Full Product Details

Author:   A. P. Monaco (Senior Scientist, ICRF, Institute of Molecular Medicine, John Radcliffe Hospital, Oxford)
Publisher:   Oxford University Press
Imprint:   Oxford University Press
Volume:   158
Dimensions:   Width: 15.40cm , Height: 1.10cm , Length: 23.30cm
Weight:   0.328kg
ISBN:  

9780199635351


ISBN 10:   0199635358
Pages:   216
Publication Date:   12 October 1995
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Out of Print
Availability:   In Print   Availability explained
Limited stock is available. It will be ordered for you and shipped pending supplier's limited stock.

Table of Contents

1. Theories of gel electrophoresis of high molecular weight DNA ; 2. PFGE in physical mapping ; 3. Mutation detection and diagnosis using PFGE ; 4. Cloning from PFGE-purified genomic DNA fragments and YACs ; 5. Yeast artificial chromosome cloning using PFGE ; 6. PFGE analysis of yeast artificial chromosomes ; 7. Functional analysis of mammalian genomes using yeast artificial chromosomes ; 8. PFGE in the study of a bacterial pathogen (Haemophilus influenzae) ; 9. Analysis of the genomes of protozoan parasites using PFGE

Reviews

All nine chapters treat all these problems which will certainly meet a great success. * Celular & Molecular Biology, Vol. 42, No. 3, 1996 * an invaluable laboratory aid for all researchers using PFGE * International Journal of Biochemistry and Cell Biology, Vol. 28, No. 7, 1996 *


`an invaluable laboratory aid for all researchers using PFGE' International Journal of Biochemistry and Cell Biology, Vol. 28, No. 7, 1996 `All nine chapters treat all these problems which will certainly meet a great success.' Celular & Molecular Biology, Vol. 42, No. 3, 1996


an invaluable laboratory aid for all researchers using PFGE International Journal of Biochemistry and Cell Biology, Vol. 28, No. 7, 1996 All nine chapters treat all these problems which will certainly meet a great success. Celular & Molecular Biology, Vol. 42, No. 3, 1996


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