Proteomics in Practice: A Guide to Successful Experimental Design

Author:   Reiner Westermeier (GE Healthcare, Munich, Germany) ,  Tom Naven (GE Healthcare, Chalfont St Giles, UK) ,  Hans-Rudolf Höpker (GE Healthcare, Freiburg, Germany)
Publisher:   Wiley-VCH Verlag GmbH
Edition:   2nd, Completely Revised Edition
ISBN:  

9783527319411


Pages:   502
Publication Date:   26 March 2008
Format:   Hardback
Availability:   To order   Availability explained
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Proteomics in Practice: A Guide to Successful Experimental Design


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Overview

Still the only concise practical guide to laboratory experiments in proteomics, this new edition now also covers DIGE technology and liquid-chromatography, while the troubleshooting section has been considerably extended. Adopting a practical approach, the authors present the relevant techniques and explain the route to successful experimental design and optimal method selection. They cover such electrophoretic techniques as isoelectric focusing, SDS page, 2-D page, and DIGE, as well as liquid-chromatography techniques, such as ion exchange, affinity chromatography and reversed-phase HPLC. Mass-spectrometric techniques include MALDI, ESI, and FT ICR. Generously illustrated, partly in color, the book also features updates of protocols as well as animations illustrating crucial methodological steps on a companion website.

Full Product Details

Author:   Reiner Westermeier (GE Healthcare, Munich, Germany) ,  Tom Naven (GE Healthcare, Chalfont St Giles, UK) ,  Hans-Rudolf Höpker (GE Healthcare, Freiburg, Germany)
Publisher:   Wiley-VCH Verlag GmbH
Imprint:   Blackwell Verlag GmbH
Edition:   2nd, Completely Revised Edition
Dimensions:   Width: 17.80cm , Height: 3.10cm , Length: 24.60cm
Weight:   1.080kg
ISBN:  

9783527319411


ISBN 10:   3527319417
Pages:   502
Publication Date:   26 March 2008
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   To order   Availability explained
Stock availability from the supplier is unknown. We will order it for you and ship this item to you once it is received by us.

Table of Contents

Preface Foreword Abbreviations Glossary of terms INTRODUCTION History Critical points Hypothesis driven approach vs. High Throughput Complementarities of workflows Concept of experimental planning Success stories, highlights of useful references PART I: PROTEOMICS TECHNOLOGY Electrophoresis Liquid Chromatography Mass spectrometry Bioinformatics tools / data mining Methods under development PART II. PRACTICAL MANUAL OF PROTEOME ANALYSIS Strategy where to start Gel-based workflow LC-based workflow Latest trends Validation with Western blotting Statistic evaluation PART III: TROUBLE SHOOTING Gel-based workflow LC based workflow References Index

Reviews

This book will be a valuable addition to any laboratory engaged in proteomics and is highly recommended. ( Internet Journal of Genomics and Proteomics 2008)


This book will be a valuable addition to any laboratory engaged in proteomics and is highly recommended. (Internet Journal of Genomics and Proteomics 2008)


Author Information

Reiner Westermeier gained his doctorate in 1981 at the Technical University of Munich, Germany. There he remained engaged in the development of new electrophoresis systems and applications until 1983, a period that also involved several international collaborations and lecturing tours. From 1984 to 1987 Reiner Westermeier was employed as an electrophoresis products and applications specialist at LKB Instrument GmbH thereafter until 1990 at Pharmacia Biotech. He founded ETC Elektrophorese-Technik in 1991 for the development of new electrophoresis methods, media, and equipment. From January 1996 to July 1997 he was manager of scientific support Europe at Pharmacia Biotech in Freiburg, Germany. He has been at Amersham Pharmacia Biotech, later GE Healthcare since July 1997, and its Senior Scientist for Proteomics since January 2002. Reiner Westermeier is the author of several publications and two books, both published by WILEY-VCH. Tom Naven and Hans-Rudolf Höpker are also working for GE Healthcare as proteomics scientists.

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