Overview

This detailed new edition presents the latest developments of the main pillars of protein analysis, namely sample preparation, separation, and characterization. Core areas in this volume are protocols for the analysis of post-translational modifications and protein interaction partners, followed by sophisticated procedures to enrich for extracellular vesicles and organelles, along with several types of protein immuno-assays complemented by various methods for the characterization of antibodies and host-cell protein analysis. Last but not least, a few standard sample preparation protocols and recent advances concerning immuno-chemical detection of proteins are included as well. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and up-to-date, Proteomic Profiling: Methods and Protocols, Second Edition serves as an ideal reference for students of biochemistry, biomedicine, biology, and genomics and will be an invaluable source for the experienced, practicing scientist as well.

Full Product Details

Author:   Anton Posch
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   2nd ed. 2021
Volume:   2261
Weight:   1.141kg
ISBN:  

9781071611883

ISBN 10:   1071611887
Pages:   592
Publication Date:   10 January 2022
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

1. How Modern Mass Spectrometry Can Solve Ancient Questions: A multi-Omics Study of the Stomach Content of the Oldest Human Ice Mummy, the 5300-Year-Old Iceman or Oetzi             Rudolf Grimm   2. Step-by-Step Sample Preparation of Proteins for Mass Spectrometric Analysis             Xinping Li, Thomas Franz, Ilian Atanassov, and Thomas Colby   3. Lab-on-a-Filter Techniques for Economical, Effective, and Flexible Proteome Analysis             Yi-Han Lin, Rodrigo Vargas Eguez, Isha Vashee, and Yanbao Yu   4. Released N-Glycan Analysis for Biotherapeutic Development Using Liquid Chromatography and Mass Spectrometry             Ximo Zhang   5. Comprehensive Protocol to Simultaneously Study Protein Phosphorylation, Acetylation, and N-Linked Sialylated Glycosylation             Marcella Nunes Melo-Braga, María Ibáñez-Vea, Katarzyna Kulej, and Martin R. Larsen   6. Phos-Tag Fluorescent Gel Staining for the Quantitative Detection of His- and Asp-Phosphorylated Proteins             Emiko Kinoshita-Kikuta, Eiji Kinoshita, and Tohru Koike   7. Polyubiquitin Profile in Down Syndrome and Alzheimer Disease Brain             Antonella Tramutola and Marzia Perluigi   8. Platform Methods to Characterize the Charge Heterogeneity of Three Common Protein Therapeutics by Imaged Capillary Isoelectric Focusing             Jiaqi Wu, Will McElroy, Charles Haitjema, Carsten Lück, and Christopher Heger   9. A Protocol for Isolation, Purification, Characterization, and Functional Dissection of Exosomes             Alin Rai, Haoyun Fang, Monique Fatmous, Bethany Claridge, Qi Hui Poh, Richard J. Simpson, and David W. Greening   10. Human Plasma Extracellular Vesicle Isolation and Proteomic Characterization for the Optimization of Liquid Biopsy in Multiple Myeloma             Antonia Reale, Tiffany Khong, Rong Xu, Maoshan Chen, Sridurga Mithraprabhu, Nicholas Bingham, Andrew Spencer, and David W. Greening   11. Isolation of Extracellular Vesicles for Proteomic Profiling             Dongsic Choi, Janusz Rak, and Yong Song Gho   12. Isolation of Proteins from Extracellular Vesicles (EVs) for Mass Spectrometry-Based Proteomic Analyses             Prabal Subedi, Michael Schneider, Michael J. Atkinson, and Soile Tapio   13. Flow Cytometry as an Important Tool in Proteomic Profiling             Michael P. Blundell, Sharon L. Sanderson, and Tracey A. Long   14. Improved Immunoprecipitation to Mass Spectrometry Method for Enrichment of Low-Abundant Protein Targets             Penny Jensen, Bhavin Patel, Suzanne Smith, Renuka Sabnis, and Barbara Kaboord   15. Multiplex Fluorescent Bead-Based Immunoassay for the Detection of Cytokines, Chemokines, and Growth Factors             Jonathan M. Preuss, Ute Burret, and Sabine Vettorazzi   16. Bead-Based Multiplex Immunoassays: Procedures, Tips, and Tricks             Candice Cox   17. Immunoaffinity-Based Liquid Chromatography Mass Spectrometric Assay to Accurately Quantify the Protein Concentration of HMGB1 in EDTA Plasma             Viktoria Anselm, Andreas Steinhilber, Cornelia Sommersdorf, and Oliver Poetz   18. Recombinant Anti-Idiotypic Antibodies in Ligand Binding Assays for Antibody Drug Development             Stefan Harth and Christian Frisch   19. cDNA Display-Mediated Immuno-PCR (cD-IPCR): An Ultrasensitive Immunoassay for Biomolecular Detection             Chathuni Jayathilake and Naoto Nemoto   20. Chromatin Immunoprecipitation (ChIP) to Study DNA-Protein Interactions             Eliza C. Small, Danielle Maryanski, Kevin J. Harvey, Michael-C. Keogh, and Andrea Johnstone   21. Profiling Protein-DNA Interactions by Chromatin Immunoprecipitation in Arabidopsis             Hendry Susila, Zeeshan Nasim, Suhyun Jin, Geummin Youn, Hyewon Jeong, Ji-Yul Jung, and Ji Hoon Ahn   22. Biotin Proximity Labeling for Protein-Protein Interaction Discovery: The BioID Method             Jeff E. Habel   23. Studying OTUD6B-OTUB1 Protein-Protein Interaction by Low-Throughput GFP-Trap Assays and High-Throughput AlphaScreen Assays             Elisabeth Weber, Kenji Schorpp, and Kamyar Hadian   24. Thermal Shift Assay for Exploring Interactions between Fatty Acid-Binding Protein and Inhibitors             Jiaqing Hao   25. Isolation and Purification of Mitochondria from Cell Culture for Proteomic Analyses             Yaschar Kabiri, Christine von Toerne, Adriana Fontes, Percy A. Knolle, and Hans Zischka   26. Investigating the Adipose Tissue Secretome: A Protocol to Generate High Quality Samples Appropriate for Comprehensive Proteomic Profiling             Sonja Hartwig, Jorg Kotzka, and Stefan Lehr   27. Methods for Proteomics-Based Analysis of the Human Muscle Secretome Using an In Vitro Exercise Model             Cora Weigert, Sonja Hartwig, and Stefan Lehr   28. Western Blotting Using In-Gel Protein Labeling as a Normalization Control: Advantages of Stain-Free Technology             Rômulo Leão Silva Neris, Andrea Marie Chua Dobles, and Aldrin V. Gomes   29. Technical Considerations for Contemporary Western Blot Techniques             Kenneth Oh   30. Simple Western: Bringing the Western Blot into the 21st Century             Carsten Lück, Charles Haitjema, and Christopher Heger   31. Development of Peptide Ligands for Targeted Capture of Host Cell Proteins from Cell Culture Production Harvests             R. Ashton Lavoie, Taufika Islam Williams, R. Kevin Blackburn, Ruben G. Carbonell, and Stefano Menegatti   32. Sample Preparation of Secreted Mammalian Host Cell Proteins and their Characterization by Two-Dimensional Electrophoresis and Western Blotting             Anton Posch, Franziska Kollmann, Thomas Berkelman, and Elizabeth Dreskin   33. Quantitative Proteomic Analysis Using Formalin-Fixed Paraffin-Embedded (FFPE) Human Cardiac Tissue             Omid Azimzade1, Mike J. Atkinson, and Soile Tapio   34. Chloroplast Isolation and Enrichment of Low Abundant Proteins by Affinity Chromatography for Identification in Complex Proteomes             Roman G. Bayer, Simon Stael, and Markus Teige   35. Principles of Protein Labeling Techniques             Christian Obermaier, Anja Griebel, and Reiner Westermeier   36. Mechanical/Physical Methods of Cell Disruption and Tissue Homogenization             Stanley Goldberg

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