Overview
This volume discusses the latest mass spectrometry (MS)-based technologies for proteoform identification, characterization, and quantification. Some of the topics covered in this book include sample preparation, proteoform separation, proteoform gas-phase fragmentation, and bioinformatics tools for MS data analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Proteoform Identification: Methods and Protocols is a valuable resource for researchers in both academia and the biopharmaceutical industry who are interested in proteoform analysis using MS.
Full Product Details
Author: Liangliang Sun , Xiaowen Liu
Publisher: Springer-Verlag New York Inc.
Imprint: Springer-Verlag New York Inc.
Edition: 1st ed. 2022
Volume: 2500
Weight: 0.678kg
ISBN: 9781071623244
ISBN 10: 1071623249
Pages: 240
Publication Date: 04 June 2022
Audience:
Professional and scholarly
,
Professional & Vocational
Format: Hardback
Publisher's Status: Active
Availability: Manufactured on demand 
We will order this item for you from a manufactured on demand supplier.
Table of Contents
Preface… Table of Contents… Contributing Authors… 1. Proteoforms and Proteoform Families: Past, Present, and Future Lloyd M. Smith 2. Membrane Ultrafiltration-Based Sample Preparation Method and Sheath-Flow CZE-MS/MS for Top-Down Proteomics Zhichang Yang and Liangliang Sun 3. Size Exclusion Chromatography Strategies and MASH Explorer for Large Proteoform Characterization Timothy N. Tiambeng, Zhijie Wu, Jake A. Melby, and Ying Ge 4. RPLC-RPLC-MS/MS for Proteoform Identification Kellye A. Cupp-Sutton, Zhe Wang, Dahang Yu, and Si Wu 5. Monolithic Materials-Based RPLC-MS for Proteoform Separation and Identification Yu Liang, Lihua Zhang, and Yukui Zhang 6. Capillary Isoelectric Focusing: Mass Spectrometry Method for the Separation and Online Characterization of Monoclonal Antibody Charge Variants at Intact and Subunit Level Jun Dai, Qiangwei Xia, and Chengjie Ji 7. Proteoform Analysis and Construction of Proteoform Families in Proteoform Suite Leah V. Schaffer, Michael R. Shortreed, and Lloyd M. Smith 8. Top-Down Mass Spectrometry Data Analysis Using TopPIC Suite In Kwon Choi and Xiaowen Liu 9. Accurate Proteoform Identification and Quantitation Using pTop 2.0 Rui-Xiang Sun, Rui-Min Wang, Lan Luo, Chao Liu, Hao Chi, Wen-Feng Zeng, and Si-Min He 10. Proteoform Identification and Quantification using Intact Protein Database Search Engine ProteinGoggle Suideng Qin and Zhixin Tian 11. Mass Deconvolution of Top-Down Mass Spectrometry Datasets by FLASHDeconv Kyowon Jeong, Jihyung Kim, and Oliver Kohlbacher 12. Deconvolving Native and Intact Protein Mass Spectra with UniDec Marius M. Kostelic and Michael T. Marty 13. Discovery of Unknown Post-Translational Modifications by Top-Down Mass Spectrometry Jesse W. Wilson and Mowei Zhou 14. Determining Copper and Zinc Content in Superoxide Dismutase using Electron Capture Dissociation under Native Spray Conditions Rachel Franklin, Michael Hare, and Joseph S. Beckman 15. Surface-Induced Dissociation for Protein Complex Characterization Sophie R. Harvey, Gili Ben-Nissan, Michal Sharon, and Vicki H. Wysocki Subject Index List…
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