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Author:   Roger A. Clegg
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   1998 ed.
Volume:   88
Dimensions:   Width: 15.20cm , Height: 1.90cm , Length: 22.90cm
Weight:   1.130kg
ISBN:  

9780896034501

ISBN 10:   089603450
Pages:   331
Publication Date:   14 January 1998
Audience:   Professional and scholarly ,  Professional & Vocational
Replaced By:   9781588297020
Format:   Paperback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

Surface Plasmon Resonance.- Microcalorimetry of Protein-Protein Interactions.- Partitioning of Proteins in Triton X-114.- Co-Immunoprecipitation.- Overlay, Ligand Blotting, and Band-Shift Techniques to Study Kinase Anchoring.- Size-Exclusion Chromatography.- Overlay and Bead Assay.- Use of GST-Fusion and Related Constructs for the Identification of Interacting Proteins.- Molecular Genetic Approaches I.- Molecular Genetic Approaches II.- Molecular Genetic Approaches III.- Molecular Genetic Approaches IV.- Use of Synthetic Peptides in the Dissection of Protein-Targeting Interactions.- SH2 and SH3 Domains.- PH Domain of Serine-Threonine Protein Kinase B (RAC-PKB).- Membrane Targeting via Protein N-Myristoylation.- Membrane Targeting via Protein Palmitoylation.- Agonist-Mediated Turnover of G-Protein ?-Subunit Palmitoyl Groups.- Membrane Targeting via Protein Prenylation.- Nuclear Protein Import in a Permeabilized Cell Assay.- Clathrin-Coated Vesicles and Targeting.- Analysis of the Sorting of Secretory Proteins to the Regulated Secretory Pathway.

Reviews

a valuable resource to the molecular biology community. The detailed protocols will greatly aid researchers - The Quarterly Review of Biology

... a valuable resource to the molecular biology community. The detailed protocols will greatly aid researchers... -The Quarterly Review of Biology

"""... a valuable resource to the molecular biology community. The detailed protocols will greatly aid researchers...""-The Quarterly Review of Biology"

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