Overview

Full Product Details

Author:   Robin Martin
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   1998 ed.
Volume:   77
Dimensions:   Width: 15.50cm , Height: 3.00cm , Length: 23.50cm
Weight:   1.860kg
ISBN:  

9780896033979

ISBN 10:   089603397
Pages:   442
Publication Date:   04 August 1998
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

Analyses of Ribosome Distribution During In Vitro Translation.- Allele-Specific Priming in the Mapping of rRNA.- Permeabilized Mammalian Cells as a System for Protein Synthesis.- The Measurement of Processivity Errors in Protein Synthesis.- A Biosynthetic Approach for the Incorporation of Unnatural Amino Acids into Proteins.- The Analysis of Translational Activity Using a Reporter Gene Constructed from Repeats of an Antibody-Binding Domain from Protein A.- In Vitro Engineering Using Acyl-Derivatized tRNAs.- In Vitro Engineering Using Synthetic tRNAs with Altered Anticodons Including Four-Nucleotide Anticodons.- Analysis of rRNA Function Using Specialized Ribosomes.- Determination of the Peptide Elongation Rate In Vivo.- The Measurement of Missense Errors During In Vivo Protein Synthesis.- Heelprinting Analysis of In Vivo Ribosome Pause Sites.- Analysis of elF-2? Kinases in Yeast.- Continuous-Flow Cell-Free Translation, Transcription-Translation, and Replication-Translation Systems.- Analysis of Translational Activity of Extracts Derived from Oocytes and Eggs of Xenopus laevis.- A Fractionated Reticulocyte Lysate System for Studies on Protein Synthesis Initiation Factors.- Measurement of Ribosomal Accuracy and Proofreading in E. coli Burst Systems.- Measurement of Rate of Protein Synthesis In Vitro.- In Vivo Mutational Analysis of Ribosomal RNA in Saccharomyces cerevisiae.- Genetic Selection of rRNA Mutations.- Toeprinting Assays.- Mapping of Pseudouridine Residues in RNA to Nucleotide Resolution.- Chemical and Enzymatic Probing of Antibiotic-Ribosome Complexes.- Protein Engineering with Nonstandard Amino Acids.- Internal Ribosome Entry Sites Tests with Circular mRNAs.- Gel Retardation and UV-Crosslinking Assays to Detect Specific RNA-Protein Interactions in the 5? or3? UTRs of Translationally Regulated mRNAs.- Translational Control by Repressor Proteins Binding to the 5?UTR of mRNAs.- Identification and Analysis of Frameshift Sites.- Synthesis and Site-Specific Binding of Thioated tRNAs to Probe Ribosome-tRNA Interactions.

Reviews

... an excellent protocols book, a copy of which should be on the shelves of every molecular biology lab. - Microbiology Today

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