Overview

How to design, execute, and interpret experiments for protein sequencing using mass spectrometry The rapid expansion of searchable protein and DNA databases in recent years has triggered an explosive growth in the application of mass spectrometry to protein sequencing. This timely and authoritative book provides professionals and scientists in biotechnology research with complete coverage of procedures for analyzing protein sequences by mass spectrometry, including step-by-step guidelines for sample preparation, analysis, and data interpretation. Michael Kinter and Nicholas Sherman present their own high-quality, laboratory-tested protocols for the analysis of a wide variety of samples, demonstrating how to carry out specific experiments and obtain fast, reliable results with a 99% success rate. Readers will get sufficient experimental detail to apply in their own laboratories, learn about the proper selection and operation of instruments, and gain essential insight into the fundamental principles of mass spectrometry and protein sequencing. Coverage includes: * Peptide fragmentation and interpretation of product ion spectra * Basic polyacrylamide gel electrophoresis * Preparation of protein digests for sequencing experiments * Mass spectrometric analysis using capillary liquid chromatography * Techniques for protein identification by database searches * Characterization of modified peptides using tandem mass spectrometry And much more

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9780471322498

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Reviews

[the authors] present...laboratory-tested protocols for the analysis of a wide variety of samples, providing sufficient detail for duplication of their methods... (SciTech Book News Vol. 25, No. 2 June 2001) ...an informative, well-written and well-illustrated book with a large number of useful references... (Spectroscopyeurope.com)

[the authors] present their own laboratory-tested protocols for the analysis of a wide variety of samples, providing sufficient detail for duplication of their methods... (SciTech Book News Vol. 25, No. 2 June 2001) ...an informative, well-written and well-illustrated book with a large number of useful references.... It should prove invaluable to anyone working in the field of protein sequencing. (Spectroscopyeurope.com)

[the authors] present...laboratory-tested protocols for the analysis of a wide variety of samples, providing sufficient detail for duplication of their methods... (SciTech Book News Vol. 25, No. 2 June 2001) ...an informative, well-written and well-illustrated book with a large number of useful references... (Spectroscopyeurope.com) ...a well-written, highly illustrative book...upper-level college students and graduate students interested in proteomics should benefit from this concise yet practical book, as would scientists making a transition into the field. (Journal of Proteome Research, Vol. 1, No. 1, January/February 2002) ...the book does a creditable job of making detailed lab protocols accessible... (The Analyst, September 2002) ...very impressive by its content and its clarity... (Biochemie, Vol.84, No.4, 2002)

[the authors] present...laboratory-tested protocols for the analysis of a wide variety of samples, providing sufficient detail for duplication of their methods... (SciTech Book News Vol. 25, No. 2 June 2001) ...an informative, well-written and well-illustrated book with a large number of useful references... (Spectroscopyeurope.com)

Author Information

MICHAEL KINTER, PhD, is an associate staff member in the Department of Cell Biology, Lerner Research Institute, Cleveland Clinic Foundation. NICHOLAS E. SHERMAN, PhD, is an assistant professor of research in the W. M. Keck Biomedical Mass Spectrometry Laboratory, Department of Microbiology, University of Virginia.

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