Overview

The third edition of this classic guide to protein purification updates methods, principles and references. As in the widely-acclaimed earlier editions, Scopes guides both the novice and the experienced researcher from theory to application. Using the book, the reader is able to integrate methods effectively into optimum protocols for the task at hand. Reviews of earlier editions: ""good practical advice that is presented in a pleasantly readable form"" --Analytical Biochemistry ""well organized and written clearly"" --American Scientist ""should be on every laboratory shelf where protein are being handled or purified...a feast and a genuine pleasure to read"" --Nature

Full Product Details

Author:   Robert K. Scopes
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   Softcover reprint of hardcover 3rd ed. 1994
Dimensions:   Width: 15.50cm , Height: 2.10cm , Length: 23.50cm
Weight:   1.250kg
ISBN:  

9781441928337

ISBN 10:   1441928332
Pages:   380
Publication Date:   06 December 2010
Audience:   Professional and scholarly ,  Professional and scholarly ,  Professional & Vocational ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

1 The Protein Purification Laboratory.- 2 Making an Extract.- 3 Analysis—Measurement of Protein and Enzyme Activity.- 4 Separation by Precipitation.- 5 Separation by Adsorption I: General Principles.- 6 Separation by Adsorption II: Ion Exchangers and Nonspecific Adsorbents.- 7 Separation by Adsorption—Affinity Techniques.- 8 Separation in Solution.- 9 Purification of Special Types of Proteins.- 10 Small-Scale and Large-Scale Procedures.- 11 Analysis for Purity.- 12 Optimization of Procedures; Final Steps.- Appendix A.- Precipitation Tables.- Appendix B.- Solutions for Measuring Protein Concentration.- Appendix C.- Buffers for Use in Protein Chemistry.- Appendix D.- Chromatographic Materials.- References.

Reviews

From reviews of earlier editions: good practical advice that is presented in a pleasantly readable form (Analytical Biochemistry), well organized and written clearly (American Scientist), should be on every laboratory shelf where protein are being handled or purified...a feast and a genuine pleasure to read (Nature)

"From reviews of earlier editions: ""good practical advice that is presented in a pleasantly readable form"" (Analytical Biochemistry), ""well organized and written clearly"" (American Scientist), ""should be on every laboratory shelf where protein are being handled or purified...a feast and a genuine pleasure to read"" (Nature)  "

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