Overview

Full Product Details

Author:   Cheryl L. Meyerkord ,  Haian Fu
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   Softcover reprint of the original 2nd ed. 2015
Volume:   1278
Weight:   1.613kg
ISBN:  

9781493949854

ISBN 10:   1493949853
Pages:   620
Publication Date:   29 October 2016
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Structural basis of protein-protein interactions.- Quantitative analysis of protein-protein interactions.- Protein-protein interaction databases.- Computational prediction of protein-protein interactions.-- Structure-based computational approaches for small-molecule modulation of protein-protein interactions.- Targeting protein-protein interactions for drug discovery.- Studying protein-protein interactions using surface plasmon resonance.- Resonant waveguide grating for monitoring bimolecular interactions.- Quartz microbalance technology for probing biomolecular interactions.- Label-free kinetic analysis of an antibody-antigen interaction using biolayer interferometry.- Characterization of protein-protein interactions by isothermal titration calorimetry.- Sedimentation equilibrium studies.- Detecting protein-protein interactions by gel filtration chromatography.- Using light scattering to determine the stoichiometry of protein complexes.- Circular dichroism (CD) analyses of protein-protein interactions.- Protein-protein interaction analysis by nuclear magnetic resonance spectroscopy.- Quantitative protein analysis by mass spectrometry.- Using peptide arrays created by the SPOT method for defining protein-protein interactions.- Fluorescence polarization assay to quantify protein-protein interactions.- Förster resonance energy transfer (FRET) microscopy for monitoring biomolecular interactions.- Utilizing ELISA to monitor protein-protein interactions.- Glutathione-S-transferase (GST)-fusion based assays for studying protein-protein interactions.- Hexahistidine (6xHis) fusion-based assays for protein-protein interactions.- Studying protein-protein interactions via blot overlay/Far Western blot.- Co-immunoprecipitation from transfected cells.- In vivo protein cross-linking.- Identification of protein-protein interactions by standard Gal4p-based yeast two-hybrid screening.- Reverse two-hybrid techniques in the yeast Saccharomyces cerevisiae .-MAPPIT, a mammalian two-hybrid method for in-cell detection of protein-protein interactions.- Bioluminescence resonance energy transfer to detect protein-protein interactions in live cells.- Mapping biochemical networks with protein fragment complementation assays.- Detection of protein-protein interaction using bimolecular fluorescence complementation assay.- Split-luciferase complementation assay to detect channel-protein interactions in live cells.- Confocal microscopy for intracellular co-localization of proteins.- Fluorescence polarization assay to quantify protein-protein interactions in an HTS format.- Estrogen receptor alpha/co-activator interaction assay - TR-FRET.- High content screening biosensor assay to identify disruptors of p53-hDM2 protein-protein interactions.- Discovery of inhibitors of the MDM2-p53 protein-protein interaction.- Biophysical methods for identifying fragment-based inhibitors of protein-protein interactions.

Reviews

Author Information

Tab Content 6

Author Website:  

Customer Reviews

Recent Reviews

No review item found!

Add your own review!

Countries Available

All regions