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Author:   A. Kristina Downing
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   Second Edition 2004
Volume:   278
Dimensions:   Width: 15.50cm , Height: 2.60cm , Length: 23.50cm
Weight:   0.874kg
ISBN:  

9781588292469

ISBN 10:   1588292460
Pages:   487
Publication Date:   17 August 2004
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

Screening and Optimizing Protein Production in E. coli.- Isotopic Labeling of Recombinant Proteins from the Methylotrophic Yeast Pichia pastoris.- Perdeuteration/Site-Specific Protonation Approaches for High-Molecular-Weight Proteins.- Segmental Isotopic Labeling for Structural Biological Applications of NMR.- TROSY-Based Correlation and NOE Spectroscopy for NMR Structural Studies of Large Proteins.- Media for Studies of Partially Aligned States.- Residual Dipolar Couplings in Protein Structure Determination.- Projection Angle Restraints for Studying Structure and Dynamics of Biomolecules.- Characterizing Domain Interfaces by NMR.- Characterization of the Overall Rotational Diffusion of a Protein From 15N Relaxation Measurements and Hydrodynamic Calculations.- TROSY-Based NMR Experiments for the Study of Macromolecular Dynamics and Hydrogen Bonding.- Measurement of Intermediate Exchange Phenomena.- NMR Studies of Partially Folded Molten-Globule States.- Structure Determination of Protein Complexes by NMR.- NMR Studies of Protein-Nucleic Acid Interactions.- Using NMRView to Visualize and Analyze the NMR Spectra of Macromolecules.- Automated NMR Structure Calculation With CYANA.- NOE Assignment With ARIA 2.0.- Membrane Protein Structure Determination Using Solid-State NMR.

Reviews

From reviews of the first edition... ...includes many good practical details which are so often omitted and which workers new to the field have to rediscover... -Analyst ...an excellent text... a very useful compilation...The limitations and power of the approaches are well presented and should help in experimental design very considerably. -Journal of Medicinal Chemistry ...a collection of excellent reviews that will form a useful contribution to the bookshelf of any NMR lab. -Trends in Biotechnology ...an excellent introduction...Readers will be left with a good understanding of the application of NMR to the study of proteins. -Quarterly Review of Biology

From reviews of the first edition includes many good practical details which are so often omitted and which workers new to the field have to rediscover - Analyst an excellent text a very useful compilation The limitations and power of the approaches are well presented and should help in experimental design very considerably. - Journal of Medicinal Chemistry a collection of excellent reviews that will form a useful contribution to the bookshelf of any NMR lab. - Trends in Biotechnology an excellent introduction Readers will be left with a good understanding of the application of NMR to the study of proteins. - Quarterly Review of Biology

From reviews of the first edition... ...includes many good practical details which are so often omitted and which workers new to the field have to rediscover... -Analyst ...an excellent text... a very useful compilation...The limitations and power of the approaches are well presented and should help in experimental design very considerably. -Journal of Medicinal Chemistry ...a collection of excellent reviews that will form a useful contribution to the bookshelf of any NMR lab. -Trends in Biotechnology ...an excellent introduction...Readers will be left with a good understanding of the application of NMR to the study of proteins. -Quarterly Review of Biology

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