Overview

A comprehensive review of the latest thinking about the molecular processes underlying conformational diseases, combined with a remarkable set of biochemical, genomic cellular, and chemical laboratory techniques for studying their genesis and pathologies. The authors apply their carefully refined methods to a variety of metabolic and neurodegenerative disorders, as well as to the aging process. The techniques presented are broadly applicable in many diverse disease contexts and may be used in both diagnosis and research on new treatment strategies.

Full Product Details

Author:   Peter Bross ,  Niels Gregersen
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2003 ed.
Volume:   232
Dimensions:   Width: 15.50cm , Height: 2.00cm , Length: 23.50cm
Weight:   1.440kg
ISBN:  

9781588290656

ISBN 10:   1588290654
Pages:   318
Publication Date:   20 June 2003
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

General Concepts and Models.- Protein Misfolding, Aggregation, and Degradation in Disease.- Basic Introduction to In Vivo Protein Folding and Its Defects.- Cystic Fibrosis.- ?1-Antitrypsin Deficiency.- Parkinson’s Disease.- Aberrant Protein Folding as the Molecular Basis of Cancer.- Yeast as a Model System to Investigate Protein Conformational Diseases.- General Methods.- Expression of Recombinant Proteins.- Expression in E. coli Systems.- Protein Expression in Yeasts.- Site-Directed Mutagenesis.- Pulse-Chase Labeling Techniques for the Analysis of Protein Maturation and Degradation.- Techniques in Conformational Disease Research.- Detection of Aggregates and Protein Inclusions by Staining of Tissues.- Study of Mutant Proteins With Folding Defects in Cultured Patient Cells.- E. coli Expression System for Identifying Folding Mutations of Human Adenosine Deaminase.- Characterization of Overexpressed Mutant Proteins in Mammalian Cells.- Investigation of Unfolded-Protein Response in Cells Expressing Familial Alzheimer's Disease-Linked Presenilin Variants.- Huntingtin Fragments Form Aggresome-Like Inclusion Bodies in Mammalian Cells.- Application of Chemical Chaperones to the Rescue of Folding Defects.- Analysis of Defective Subunit Interactions Using the Two-Hybrid System.- Investigation of Folding and Degradation of In Vitro Synthesized Mutant Proteins in the Cytosol.- Investigation of Folding and Degradation of In Vitro Synthesized Mutant Proteins in Microsomes.- Investigation of Folding and Degradation of In Vitro Synthesized Mutant Proteins in Mitochondria.- Investigation of Folding and Degradation of Mutant Proteins Synthesized in Semipermeabilized Cells.

Reviews

...a very sound book that provides a solid background in conformational disease and presents the reader with helpful experimental protocols for studying protein folding folding and misfolding. - Pharmaceutical Research ...this book will be a very useful took for specialists in protein chemistry, molecular biology and basic medicine, and also for teachers and their students studying principles of proteomics. Biochemistry

.,. a very sound book that provides a solid background in conformational disease and presents the reader with helpful experimental protocols for studying protein folding folding and misfolding. - Pharmaceutical Research <br> .,. this book will be a very useful took for specialists in protein chemistry, molecular biology and basic medicine, and also for teachers and their students studying principles of proteomics. Biochemistry

...a very sound book that provides a solid background in conformational disease and presents the reader with helpful experimental protocols for studying protein folding folding and misfolding. - Pharmaceutical Research ...this book will be a very useful took for specialists in protein chemistry, molecular biology and basic medicine, and also for teachers and their students studying principles of proteomics. Biochemistry

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