Overview

Full Product Details

Author:   Peter Bross ,  Niels Gregersen ,  Niels Gregersen
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2010 ed.
Volume:   648
Dimensions:   Width: 17.80cm , Height: 2.50cm , Length: 25.40cm
Weight:   0.923kg
ISBN:  

9781607617556

ISBN 10:   1607617552
Pages:   330
Publication Date:   19 August 2010
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Awaiting stock  
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Table of Contents

Concepts.- Protein Misfolding and Cellular Stress: An Overview.- Protein Aggregation Diseases: Toxicity of Soluble Prefibrillar Aggregates and Their Clinical Significance.- Consequences of Stress in the Secretary Pathway: The ER Stress Response and Its Role in the Metabolic Syndrome.- What Role Does Mitochondrial Stress Play in Neurodegenerative Diseases?.- Autophagy in Disease.- Mitophagy and Mitoptosis in Disease Processes.- Cellular Stress and Protein Misfolding During Aging.- Measuring Consequences of Protein Misfolding and Cellular Stress Using OMICS Techniques.- Protocols.- Production of Cells with Targeted Integration of Gene Variants of Human ABC Transporter for Stable and Regulated Expression Using the Flp Recombinase System.- Stress Response Profiles in Human Fibroblasts Exposed to Heat Shock or Oxidative Stress.- Examining Ubiquitinated Protein Aggregates in Tissue Sections.- Determination of Proteasomal Activities.- Measurement of Autophagy in Cells and Tissues.- Transcription Factor Sequestration by Polyglutamine Proteins.- Biological Membranes as Protein Aggregation Matrices and Targets of Amyloid Toxicity.- Measurement of Mitochondrial ROS Production.- In Vivo Detection of Oxidized Proteins: A Practical Approach to Tissue-Derived Mitochondria.- Measurement of Oxidized/Reduced Glutathione Ratio.- Determination of Altered Mitochondria Ultrastructure by Electron Microscopy.- Assessing Bad Sub-cellular Localization Under Conditions Associated with Prevention or Promotion of Mitochondrial Permeability Transition-Dependent Toxicity.- A Cellular Viability Assay to Monitor Drug Toxicity.- Rescue of Misfolded Proteins and Stabilization by Small Molecules.

Reviews

From the reviews: Methods in Molecular Biology deals with current topics and techniques in cellular stress as well as methods to study and analyze the effects of cellular stress on protein misfolding. ! The book is written mainly for scientists involved in the study of diseases resulting from protein misfolding. ! the protocol is straightforward and is based on proven examples that render these diverse methods credible. This should prove to be a valuable companion to all researchers in the field of cellular stress and protein misfolding. (Ruben Mestril, Doody's Review Service, February, 2011)

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