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Full Product Details

Author:   Kristian Müller ,  Katja Arndt
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2007 ed.
Volume:   352
Dimensions:   Width: 15.20cm , Height: 2.70cm , Length: 22.90cm
Weight:   0.687kg
ISBN:  

9781588290724

ISBN 10:   1588290727
Pages:   328
Publication Date:   15 September 2006
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

Design and Computational Strategies for Protein Engineering.- Combinatorial Protein Design Strategies Using Computational Methods.- Global Incorporation of Unnatural Amino Acids in Escherichia coli.- Considerations in the Design and Optimization of Coiled Coil Structures.- Calcium Indicators Based on Calmodulin-Fluorescent Protein Fusions.- Design and Synthesis of Artificial Zinc Finger Proteins.- Monobodies.- Engineering Site-Specific Endonucleases.- Evolutionary Strategies for Protein Engineering.- Protein Library Design and Screening.- Protein Design by Binary Patterning of Polar and Nonpolar Amino Acids.- Versatile DNA Fragmentation and Directed Evolution With Nucleotide Exchange and Excision Technology.- Degenerate Oligonucleotide Gene Shuffling.- M13 Bacteriophage Coat Proteins Engineered for Improved Phage Display.- Ribosome-Inactivation Display System.- Compartmentalized Self-Replication.- Synthesis of Degenerated Libraries of the Ras-Binding Domain of Raf and Rapid Selection of Fast-Folding and Stable Clones With the Dihydrofolate Reductase Protein Fragment Complementation Assay.- A General Method of Terminal Truncation, Evolution, and Re-Elongation to Generate Enzymes of Enhanced Stability.

Reviews

From the reviews: <p> Whether the research in your laboratory focuses on protein engineering or you wish to adopt a method for engineering a protein of interest, you will find this book, with it up-to-date stepwise protocols and experimentally oriented language, well suited to these goals. -Quarterly Review of Biology <p> The chapters of Protein Engineering Protocols have been written by researchers with years of practical experience at the bench. It represents a methodological a ~cook-booka (TM) of high quality. The diverse topics are well written and covered in an up-to-date manner. This makes it a bench-top handbook indispensable for all scientists who either work directly in protein engineering and design or use the described approaches of this field for their own research. (Nediljko Budisa, ChemBioChem, Vol. 8, 2007)

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