Overview

Site-specific mutagenesis of DNA, developed some thirty years ago, has proven to be one of the most important advances in biology. By allowing the site-specific replacement of any amino acid in a protein with one of the other nineteen amino acids, it ushered in the new era of ""Protein Engineering"". The field of protein engineering has, however, evolved rapidly since then and the last fifteen years have witnessed remarkable advances through the use of new chemical, biochemical and molecular biological tools towards the synthesis and manipulation of proteins. The chapters included in this book reflect the rapid evolution of protein engineering and its many applications in basic research, biotechnology, material sciences and therapy. This book will provide the reader with an introduction to state-of the-art concepts and methods and will be of use to anyone interested in the study of proteins, in academia as well as in industry.

Full Product Details

Author:   Caroline Koehrer ,  Uttam L. RajBhandary
Publisher:   Springer-Verlag Berlin and Heidelberg GmbH & Co. KG
Imprint:   Springer-Verlag Berlin and Heidelberg GmbH & Co. K
Edition:   Softcover reprint of hardcover 1st ed. 2009
Volume:   22
Dimensions:   Width: 15.50cm , Height: 1.80cm , Length: 23.50cm
Weight:   0.551kg
ISBN:  

9783642089923

ISBN 10:   3642089925
Pages:   347
Publication Date:   25 November 2010
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Out of print, replaced by POD  
We will order this item for you from a manufatured on demand supplier.

Table of Contents

Understanding Enzyme Mechanism through Protein Chimeragenesis.- Chemical Protein Engineering: Synthetic and Semisynthetic Peptides and Proteins.- Native Chemical Ligation: SemiSynthesis of Post-translationally Modified Proteins and Biological Probes.- Chemical Methods for Mimicking Post-Translational Modifications.- Noncanonical Amino Acids in Protein Science and Engineering.- Fidelity Mechanisms of the Aminoacyl-tRNA Synthetases.- Specialized Components of the Translational Machinery for Unnatural Amino Acid Mutagenesis: tRNAs, Aminoacyl-tRNA Synthetases, and Ribosomes.- In Vivo Studies of Receptors and Ion Channels with Unnatural Amino Acids.- Synthesis of Modified Proteins Using Misacylated tRNAs.- Cell-Free Synthesis of Proteins with Unnatural Amino Acids. The PURE System and Expansion of the Genetic Code.- Engineering Nucleobases and Polymerases for an Expanded Genetic Alphabet.- Understanding Membrane Proteins. How to Design Inhibitors of Transmembrane Protein—Protein Interactions.

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