Overview

Protein engineering is the rational modification or redesign of proteins using genetic engineering. Thus, it is now possible to modify enzyme specificities, remodel antibodies, and redesign many multi-domain proteins for therapeutic purposes. While the procedures for the introduction of mutations have become routine, predicting and understanding the effects of these mutations can be complicated. This volume provides a comprehensive guide to the methods used at every stage of the engineering process, from the choice of mutation strategy, through protein stability studies, to critical evaluations of mammalian, yeast, and bacterial host expression systems. Protein Engineering: A Practical Approach is the first practical guide to this fascinating mixture of molecular biology, protein structure analysis, computation, and biochemistry. It combines a thorough theoretical foundation with detailed protocols and will be invaluable to all research workers in the area, from graduate students to senior investigators.

Full Product Details

Author:   Anthony R. Rees (Professor, Department of Biochemistry, Professor, Department of Biochemistry, University of Bath) ,  Michael J. E. Sternberg (, Imperial Cancer Research Fund, London) ,  Ronald Wetzel (, Smith Kline Beecham, Pennsylvania, USA) ,  R. Wetzel (Smith, Kline and French, Pennsylvania, USA)
Publisher:   Oxford University Press
Imprint:   Oxford University Press
Volume:   108
Dimensions:   Width: 15.60cm , Height: 2.30cm , Length: 23.40cm
Weight:   0.646kg
ISBN:  

9780199631384

ISBN 10:   0199631387
Pages:   424
Publication Date:   10 December 1992
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Postgraduate, Research & Scholarly
Format:   Paperback
Publisher's Status:   Active
Availability:   To order  
Stock availability from the supplier is unknown. We will order it for you and ship this item to you once it is received by us.

Table of Contents

1. Methods of structural analysis of proteins - part 1 - protein crystallography ; 2. Methods of structural analysis of proteins - part 2 - nuclear magnetic resonance ; 3. Molecular sequence data- bases and their uses ; 4. Design of protein structures ; 5. From protein sequence to structure ; 6. The use of theoretical methods in protein engineering ; 7. Principles of protein stability - part 1 - reversible unfolding of proteins: kinetic and thermodynamic analysis ; 8. Principles of protein stability - part 2 - enhanced folding and stabilization of proteins by suppression of aggregation in vitro and in vivo ; 9. Principles of protein turnover: possible manipulations ; 10. Chemical approaches to expression of proteins ; 11. Protein engineering of antibody combining sites ; 12. Selection of variants of antibodies and other protein molecules using display on the surface of bacteriophage fd ; 13. Expression of proteins in prokaryotic systems - principles and 'case' studies ; 14. Expression of proteins in yeast ; 15. Genetic engineering of the processing and secretory pathway of an inflammatory cytokine in mammalian cells: a case study ; 16. Industrial applications of protein engineering and scale-up of protein production: l-antitrypsin, a case study ; Appendix

Reviews

'the first practical guide to this fascinating area of science ... the major parts of the book are very well written and of great didactic value ... The book is recommended for undergraduate and graduate students and to researchers in the field of protein engineering.' Arvind M. Kayastha, Biochemical Education 21(4) 1993 'The problem is to produce a specific purified protein in significant quantities. This book provides some practical details that can help in this task.' International Journal of Biochemistry, Vol. 25, No. 12, 1993 'Protein Engineering: A Practical Approach is the first reference book in this network of molecular biology, structure of proteins and biochemistry. It combines theretical background and detailed procedures; both together enable researchers in this field to plan and carry out experiments for their own projects. This book should therefore serve as the standard text for students and scientists in the field of protein engineering.' Arzneimittel-Forschung/Drug Research Issue 12/93

'Protein Engineering: A Practical Approach is the first reference book in this network of molecular biology, structure of proteins and biochemistry. It combines theretical background and detailed procedures; both together enable researchers in this field to plan and carry out experiments for their own projects. This book should therefore serve as the standard text for students and scientists in the field of protein engineering.' Arzneimittel-Forschung/Drug Research Issue 12/93 'The problem is to produce a specific purified protein in significant quantities. This book provides some practical details that can help in this task.' International Journal of Biochemistry, Vol. 25, No. 12, 1993 'the first practical guide to this fascinating area of science ... the major parts of the book are very well written and of great didactic value ... The book is recommended for undergraduate and graduate students and to researchers in the field of protein engineering.' Arvind M. Kayastha, Biochemical Education 21(4) 1993

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