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OverviewLeading practitioners authoritatively describe the newest and most effective spectrometric techniques for the analysis of proteins and peptides. The areas covered range from the elucidation of primary and secondary protein structure and the rapid identification of proteins using database techniques to methods for sequencing, as well as methods for the quantitative determination of peptides. Other chapters provide detailed information on the analysis of glycoproteins and glycopeptides and on the use of mass spectrometry to probe the interactions of proteins, both covalent and noncovalent. Full Product DetailsAuthor: John R. ChapmanPublisher: Humana Press Inc. Imprint: Humana Press Inc. Edition: 1996 ed. Volume: 61 Dimensions: Width: 15.50cm , Height: 2.00cm , Length: 23.50cm Weight: 1.510kg ISBN: 9780896033450ISBN 10: 0896033457 Pages: 350 Publication Date: 19 August 1996 Audience: College/higher education , Professional and scholarly , Undergraduate , Professional & Vocational Format: Hardback Publisher's Status: Active Availability: Out of print, replaced by POD  We will order this item for you from a manufatured on demand supplier. Table of ContentsMass Spectrometry in the Analysis of Peptides and Proteins, Past and Present.- Mass Spectrometry.- Charged Derivatives for Peptide Sequencing Using a Magnetic Sector Instrument.- New Instrumental Approaches to Collision-Induced Dissociation Using a Time-of-Flight Instrument.- Mass Spectrometric Quantification of Neuropeptides.- The Identification of Electrophoretically Separated Proteins by Peptide Mass Fingerprinting.- Liquid Chromatography/Mass Spectrometry for the Analysis of Protein Digests.- Structural Analysis of Protein Variants.- Noncovalent Interactions Observed Using Electrospray Ionization.- Protein Secondary Structure Investigated by Electrospray Ionization.- The Effect of Detergents on Proteins Analyzed by Electrospray Ionization.- Posttranslational Modifications Analyzed by Automated Protein Ladder Sequencing.- Rapid Analysis of Single-Cysteine Variants of Recombinant Proteins.- Disulfide Bond Location in Proteins.- Determination of Loading Values and Distributions for Drugs Conjugated to Proteins and Antibodies by MALDI-MS and ESI-MS.- Hydrophobic Proteins and Peptides Analyzed by Matrix-Assisted Laser Desorption/Ionization.- Analysis of Glycoproteins and Glycopeptides Using Fast-Atom Bombardment.- Identification of Cleaved Oligosaccharides by Matrix-Assisted Laser Desorption/Ionization.- Structural Characterization of Protein Glycosylation Using HPLC/Electrospray Ionization Mass Spectrometry and Glycosidase Digestion.- Analysis of Complex Protein and Glycoprotein Mixtures by Electrospray Ionization Mass Spectrometry with Maximum Entropy Processing.- The Use of Databases in Searching the Literature of Biological Mass Spectrometry.ReviewsThe 21 Chapters cover the whole range of techniques, with enough detail about the physical and technical background to satisfy and enable the critical user without swamping him or her; all illustrated with a wide range of cutting-edge applications...The book closes with a valuable series of appendices (acronyms, mass and abundance values, fragmentation patterns etc)...there is no lightweight material or padding in this important and timely book-The European Peptide Society Newsletter ...useful to newcomers. - SGM Quarterly The 21 Chapters cover the whole range of techniques, with enough detail about the physical and technical background to satisfy and enable the critical user without swamping him or her; all illustrated with a wide range of cutting-edge applications...The book closes with a valuable series of appendices (acronyms, mass and abundance values, fragmentation patterns etc)...there is no lightweight material or padding in this important and timely book - The European Peptide Society Newsletter useful to newcomers. - SGM Quarterly The 21 Chapters cover the whole range of techniques, with enough detail about the physical and technical background to satisfy and enable the critical user without swamping him or her; all illustrated with a wide range of cutting-edge applications...The book closes with a valuable series of appendices (acronyms, mass and abundance values, fragmentation patterns etc)...there is no lightweight material or padding in this important and timely book-The European Peptide Society Newsletter ...useful to newcomers. - SGM Quarterly The 21 Chapters cover the whole range of techniques, with enough detail about the physical and technical background to satisfy and enable the critical user without swamping him or her; all illustrated with a wide range of cutting-edge applications...The book closes with a valuable series of appendices (acronyms, mass and abundance values, fragmentation patterns etc)...there is no lightweight material or padding in this important and timely book-The European Peptide Society Newsletter ...useful to newcomers. - SGM Quarterly Author InformationTab Content 6Author Website:Countries AvailableAll regions |
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