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Full Product Details

Author:   Andrew F. Hill
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2008 ed.
Volume:   459
Dimensions:   Width: 15.50cm , Height: 1.80cm , Length: 23.50cm
Weight:   0.612kg
ISBN:  

9781588298973

ISBN 10:   1588298973
Pages:   280
Publication Date:   30 May 2008
Audience:   College/higher education ,  Postgraduate, Research & Scholarly
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

Cell Culture Models to Unravel Prion Protein Function and Aberrancies in Prion Diseases.- Investigation of PrPC Metabolism and Function in Live Cells.- Immunodetection of PrPSc Using Western and Slot Blotting Techniques.- Assaying Prions in Cell Culture.- Generation of Cell Lines Propagating Infectious Prions and the Isolation and Characterization of Cell-derived Exosomes.- Neurotoxicity of Prion Peptides on Cultured Cerebellar Neurons.- Understanding the Nature of Prion Diseases Using Cell-free Assays.- Methods for Conversion of Prion Protein into Amyloid Fibrils.- Amplification of Purified Prions In Vitro.- Expression and Purification of Full-Length Recombinant PrP of High Purity.- Analysis of PrP Conformation Using Circular Dichroism.- Effect of Copper on the De Novo Generation of Prion Protein Expressed in Pichia pastoris.- Biophysical Investigations of the Prion Protein Using Electron Paramagnetic Resonance.- Molecular Diagnosis of Human Prion Disease.- Analysis of Endogenous PrPC Processing in Neuronal and Non-neuronal Cell Lines.- Molecular Typing of PrPres in Human Sporadic CJD Brain Tissue.- Transgenic Mouse Models of Prion Diseases.- Quantitative Bioassay of Surface-bound Prion Infectivity.

Reviews

From the reviews: Interspersed are concise reviews focusing on current growth areas in the prion field. ! The protocols are clear and authoritative. For those entering the field it would be an excellent guide. (Hugh Pennington, Microbiology Today, February, 2009)

From the reviews: Interspersed are concise reviews focusing on current growth areas in the prion field. ... The protocols are clear and authoritative. For those entering the field it would be an excellent guide. (Hugh Pennington, Microbiology Today, February, 2009)

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