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Author:   Hans Bisswanger
Publisher:   Wiley-VCH Verlag GmbH
Imprint:   Wiley-VCH Verlag GmbH
Dimensions:   Width: 16.90cm , Height: 1.40cm , Length: 24.10cm
Weight:   0.534kg
ISBN:  

9783527304448

ISBN 10:   3527304444
Pages:   272
Publication Date:   22 June 2004
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Out of Print
Availability:   Out of stock  

Table of Contents

Abbreviations.1 Introduction.2 Enzyme Nomenclature.3 Enzyme Reactions.3.1 Theory of the Enzyme Reaction.3.1.1 Reaction Order.3.1.1.1 Zero-order Reactions.3.1.1.2 First-order Reactions.3.1.1.3 Second- and Higher-order Reactions.3.2 Michaelis-Menten Equation.3.3 Theory of the Enzyme Assay.3.3.1 Analysis of Progress Curves.3.3.2 How To Develop an Enzyme Assay.3.3.2.1 General Considerations.3.3.2.2 pH.3.3.2.3 Ionic Strength and Buffer.3.3.2.4 Temperature.3.3.2.5 Special Components.3.3.2.6 Concentration of Components and Technical Implications.3.3.3 Enzyme Activity.3.3.3.1 Definition of Enzyme Units.3.3.3.2 Absorption Coefficient.3.3.3.3 Calculation of Enzyme Activity.3.4 Theory of Coupled Enzyme Reactions.3.4.1 Two Coupled Reactions.3.4.2 Three Coupled Enzyme Reactions.3.5 Substrate Determination.3.5.1 End-point Method.3.5.2 Coupled Enzyme Reactions.3.5.3 Kinetic Method for Substrate Determination.3.5.4 Enzymatic Cycling.3.6 Instrumental Aspects.3.6.1 Spectroscopic Methods.3.6.1.1 UV/Visible Photometry.3.6.1.2 Turbidity Measurements.3.6.1.3 Fluorimeter.3.6.1.4 Luminometry.3.6.1.5 Polarimetry.3.6.2 Electrochemical Measurements.3.6.2.1 pH Meter.3.6.2.2 pH Stat.3.6.2.3 Potentiometry.3.6.3 Analysis of Reactions that Release or Consume Gas.3.6.3.1 Warburg Manometer.3.6.3.2 Radioactive Labeling.3.6.3.3 Oxygen and Carbon Dioxide Electrodes.3.7 Enzyme Assays.3.7.1 General Considerations.3.7.2 Practical Aspects.3.7.3 Buffers and Solutions.3.7.3.1 Theoretical Aspects.3.7.3.2 Preparation of Buffers.3.7.3.3 Frequently Used Buffers and Solutions.3.7.3.4 Calculation of Enzyme Activities.3.7.4 Oxidoreductase Assays.3.7.4.1 Optical Assays.3.7.4.2 Fluorimetric Assays.3.7.4.3 Alcohol Dehydrogenase.3.7.4.4 Shikimate Dehydrogenase.3.7.4.5 l-Lactate Dehydrogenase.3.7.4.6 Isocitrate Dehydrogenase.3.7.4.7 Glucose-6-phosphate Dehydrogenase.3.7.4.8 Malate Dehydrogenase.3.7.4.9 Glucose Oxidase.3.7.4.10 Glyceraldehyde-3-phosphate Dehydrogenase.3.7.4.11 Pyruvate Ferredoxin Oxidoreductase.3.7.4.12 Glutamate Dehydrogenase.3.7.4.13 l-Amino Acid Oxidase.3.7.4.14 Uricase.3.7.4.15 Catalase.3.7.4.16 Peroxidase.3.7.4.17 Luciferase.3.7.5 Pyruvate Dehydrogenase Complex (PDHC).3.7.5.1 Overall Activity of PDHC by NADt Reduction.3.7.5.2 Overall Activity of PDHC by Dismutation Assay.3.7.5.3 Pyruvate Dehydrogenase (Lipoamide).3.7.5.4 Dihydrolipoamide Acetyltransferase.3.7.5.5 Dihydrolipoamide Dehydrogenase.3.7.6 alpha-Oxoglutarate Dehydrogenase Complex (OGDHC).3.7.6.1 Overall Activity by NADt Reduction.3.7.6.2 alpha-Oxoglutarate Dehydrogenase.3.7.7 Transferases.3.7.7.1 Fatty Acid Synthase.3.7.7.2 Phosphorylase a.3.7.7.3 Hexokinase.3.7.7.4 Pyruvate Kinase.3.7.7.5 Acetate Kinase.3.7.7.6 3-Phosphoglycerate Kinase.3.7.8 Hydrolases.3.7.8.1 Lipase.3.7.8.2 Cholinesterase.3.7.8.3 Acetylcholinesterase.3.7.8.4 Alkaline Phosphatase.3.7.8.5 Acid Phosphatase.3.7.8.6 Ribonuclease (Pancreatic).3.7.8.7 alpha-Amylase.3.7.8.8 Amyloglucosidase.3.7.8.9 Lysozyme.3.7.8.10 alpha-Glucosidase.3.7.8.11 beta-Galactosidase.3.7.8.12 beta-Fructosidase.3.7.9 Proteases.3.7.9.1 Anson Assay.3.7.9.2 Casein Assay.3.7.9.3 Azocasein Assay.3.7.9.4 Ninhydrin Assay.3.7.9.5 Leucine Aminopeptidase.3.7.9.6 Chymotrypsin.3.7.9.7 Pepsin.3.7.9.8 Trypsin.3.7.9.9 Asparaginase.3.7.9.10 Urease.3.7.9.11 Adenosine Triphosphatase.3.7.10 Lyases.3.7.10.1 Pyruvate decarboxylase.3.7.10.2 Aldolase.3.7.10.3 Anthranilate Synthase.3.7.10.4 Carbonic Anhydrase.3.7.10.5 Fumarase.3.7.11 Isomerases.3.7.11.1 Glucose/Xylose isomerase.3.7.12 Determination of Nicotinamide Nucleotides by Enzymatic Cycling.3.7.12.1 Determination of NADP(H).3.7.12.2 Determination of NAD(H).3.8 Diverse Methods.3.8.1 Protein Determination.3.8.1.1 Biuret Method.3.8.1.2 BCA Assay.3.8.1.3 Lowry Assay.3.8.1.4 Coomassie Binding Assay (Bradford Assay).3.8.1.5 Absorption Method.3.8.1.6 Fluorimetric Assay.3.8.1.7 Ninhydrin Assay.3.8.1.8 Modified Ninhydrin Assay for Protein Determination without Hydrolysis.3.8.1.9 Protein Determination with 2-Hydroxy-1-naphthaldehyde.3.8.2 Phosphate Determination.3.8.3 Concentration of Enzyme Solutions.3.8.3.1 Precipitation.3.8.3.2 Ultrafiltration and Dialysis.3.8.3.3 Ultracentrifugation.3.8.3.4 Lyophilization.3.8.3.5 Miscellaneous Techniques.3.9 Enzyme Immunoassays.3.9.1 Non-competitive Solid-phase Enzyme Immunoassay.3.9.2 Competitive, Solid-phase Enzyme Immunoassay.3.9.3 Methods for Enzyme Immunoassays and Immobilization Techniques.3.9.3.1 Protein Coupling to Cyanogen Bromide-activated Agarose.3.9.3.2 Coupling of Diaminohexyl Spacer.3.9.3.3 Periodate Activation of Cellulose.3.9.3.4 Conjugation of Proteins (Antibodies) to Enzymes (Peroxidase).3.9.3.5 Introduction of Thiol Groups into Antibodies or Proteins.3.9.3.6 Conjugation of b-galactosidase to Antibodies by MBS.3.9.3.7 Conjugation of Alkaline Phosphatase to Antibodies by Glutaraldehyde.3.9.3.8 Cross-linking of Proteins with Dimethyl Suberimidate.4 Binding Measurements.4.1 Irreversible, Reversible, Specific, and Unspecific Binding.4.1.1 General Considerations.4.1.2 Experimental Aspects.4.1.2.1 Amount of Enzyme or Macromolecule.4.2.1.2 Stability of the Enzyme or Macromolecule.4.1.2.3 Stability of the Ligand.4.1.2.4 Concentration of Components.4.2 Binding Measurement by Size Discrimination.4.2.1 Ultrafiltration.4.2.2 Equilibrium Dialysis.4.2.2.1 Determination of Indole Binding to Bovine Serum Albumin.4.2.3 Evaluation of Binding Experiments.4.2.4 Gel Filtration.4.2.5 Ultracentrifugation.4.3 Spectroscopic Techniques.4.3.1 Difference Spectroscopy.4.3.1.1 Difference Spectroscopic Determination of Binding of Ligands to Catalase.4.3.1.2 Evaluation of Spectroscopic Binding Curves.4.3.2 Fluorescence Spectroscopy.4.3.2.1 ANS Binding to Bovine Serum Albumin.4.4 Other Methods for Binding Measurements.4.4.1 Radioactive Labeling.4.4.2 Reflectrometric Interference Spectroscopy.5 Enzymes in Technical Applications.5.1 Principles of Enzyme Immobilization.5.1.1 Adsorption.5.1.2 Entrapment.5.1.3 Encapsulation.5.1.4 Cross-linking.5.1.5 Covalent Binding to Solid Supports.5.1.5.1 Supports.5.1.5.2 Spacer.5.2 Methods for Enzyme Immobilization.5.2.1 Microencapsulation inside Nylon Beads.5.2.2 Entrapment in Polyacrylamide.5.2.3 Covalent Immobilization of Enzymes on Nonporous Glass Surfaces.5.2.4 Immobilization on Controlled-pore Glass.5.2.5 Covalent Immobilization of Enzymes to Polyamide.5.2.6 O-Alkylation with Triethyloxoniumtetrafluoroborate.5.2.7 Immobilization of Enzymes to Amine Groups after Partial Hydrolysis of Polyamide.5.2.8 Immobilization of Enzymes to Carboxyl Groups after Partial Hydrolysis of Polyamide.5.2.9 Immobilization of Enzymes to Polyester.5.2.10 Immobilization by Alkaline Hydrolysis and Activation with Tosylchloride.5.2.11 Alkaline Hydrolysis and Activation by Carbonyldiimidazol.5.3 Analysis of Immobilized Enzymes.5.3.1 Protein Assays.5.3.2 Enzyme Assays.5.3.2.1 Modified Optical Assay for Immobilized Enzymes.5.3.2.2 Cofactors in Reactions with Immobilized Enzymes.5.4 Enzyme Reactors.5.4.1 Batch Reactor (Stirred-tank Reactor).5.4.2 Membrane Reactor.5.4.3 Solid-bed Reactor.5.4.4 Immobilized Cells.5.5 Biosensors.5.5.1 Enzyme Electrodes.5.5.2 Immunoelectrodes.5.5.3 Other Biosensors.5.5.4 Bioaffinity Sensors.5.6 Immobilized Enzymes in Therapy.Index.

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Buchkurzvorstellung mit farbigem Cover in: <br> Process August 2004 <br> New Drugs, November 2004 <br> CEABA-VtB, Literaturdatenbank der Dechema <br> In Summa hat der Autor mit >Practical EnzymologyEnzymkinetik Kai Tittmann, Halle, Nachrichten aus der Chemie, 05/2005 <br>

Buchkurzvorstellung mit farbigem Cover in: Process August 2004 New Drugs, November 2004 CEABA-VtB, Literaturdatenbank der Dechema In Summa hat der Autor mit >Practical EnzymologyEnzymkinetik Kai Tittmann, Halle, Nachrichten aus der Chemie, 05/2005 Buchkurzvorstellung mit farbigem Cover in: Process August 2004

Buchkurzvorstellung mit farbigem Cover in: Process August 2004 New Drugs, November 2004 CEABA-VtB, Literaturdatenbank der Dechema In Summa hat der Autor mit >Practical EnzymologyEnzymkinetik Kai Tittmann, Halle, Nachrichten aus der Chemie, 05/2005

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