Overview

Deals with biotechnological approaches incorporated into po- tato improvement progammes. These methods have far-reaching implications for the synthesis of improved, disease- resist- ant and nutritious cultivars of potato.

Full Product Details

Author:   Professor Dr. Y. P. S. Bajaj
Publisher:   Springer-Verlag Berlin and Heidelberg GmbH & Co. KG
Imprint:   Springer-Verlag Berlin and Heidelberg GmbH & Co. K
Volume:   3
Weight:   1.125kg
ISBN:  

9783540179665

ISBN 10:   3540179666
Pages:   509
Publication Date:   27 October 1987
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Postgraduate, Research & Scholarly
Format:   Hardback
Publisher's Status:   Active
Availability:   Out of stock  
The supplier is temporarily out of stock of this item. It will be ordered for you on backorder and shipped when it becomes available.

Table of Contents

Section I Micropropagation, Virus-Free Plants, Haploid Production and Field Trials.- I.1 Biotechnology and 21st Century Potato.- 1. General Account.- 2. Traditional Methods of Potato Breeding.- 3. Biotechnology Produces Novel Potatoes.- 4. The Future Potato.- References.- I.2 In Vitro Propagation of Potato - Progress in Czechoslovakia.- 1. Introduction.- 2. Meristem Tip Culture.- 3. In Vitro Clonal Propagation.- References.- I.3 Virus-Free Potatoes Through Meristem Culture.- 1. Introduction.- 2. Virus Elimination.- 3. Indexing Source Plants.- 4. Heat Therapy.- 5. Culture Medium.- 6. Meristem Culture.- 7. Indexing Treated Plantlets.- 8. Elimination of PSTV.- 9. Indexing Field Plants.- 10. Summary.- References.- I.4 In Vitro Induction of Virus-Free Potatoes by Chemotherapy.- 1. Introduction.- 2. Meristem and Explant Culture of Potato in the Presence of Virazole.- 3. Discussion.- References.- I.5 In Vitro Improvement of Potatoes: The New Zealand Approach.- 1. Introduction.- 2. Biotechnological Approaches.- 3. Future Prospects of Biotechnology in Potatoes in New Zealand.- References.- I.6 In Vitro Production and Release of Potato Varieties in China.- 1. Introduction.- 2. Meristem-Tip Culture.- 3. Cold Storage of Shoot Tips and Plantlets.- 4. Large-Scale Propagation and Growing of the Virus-Free Plantlets.- 5. Varieties Released.- 6. Comparison of Growth and Production of Healthy and Infected Plants.- 7. Conclusions and Prospects.- References.- I.7 Field Performance of Micropropagated Potato Plants.- 1. Introduction.- 2. Growth and Production of Micropropagated Potatoes as Compared to Tuber-Generated Potatoes.- 3. Timing and Handling of Micropropagules.- 4. Conclusions.- References.- I.8 Anther Culture and Haploid Production in Potato.- 1. Introduction.- 2. Factors Influencing Anther Culture.- 3. Pollen Culture.- 4. Development of Pollen Grains to Plants.- 5. Utilization of Anther Culture Technique and Haploids.- References.- Section II Physiological, Biochemical and Nutritional Studies, and Molecular Genetics.- II. 1 Potato Tuber Storage: Biochemical and Physiological Changes.- 1. Introduction.- 2. Relations Between Storage and Plant Hormones.- 3. Changes in Various Constituents During Storage.- 4. Changes in Metabolic Activities with Storage.- References.- II.2 Biotechnology of Nutritional Improvement of Potato.- 1. Potato as a Food.- 2. Potato as a Source of Energy.- 3. Composition of Potato.- 4. Nutritional Considerations on Improvement.- 5. Conventional Methods of Improvement of Proteins and Amino Acids.- 6. Biotechnological Approaches to Nutritional Improvement.- 7. Conclusions and Prospects.- References.- II.3 Molecular Genetics of Potato.- 1. Introduction.- 2. Nuclear Genetics.- 3. Organelle Genetics.- 4. DNA-Mediated Genetic Transformation in S. tuberosum.- 5. Molecular Genetics of Somaclonal Variation.- 6. Conclusions and Prospects.- References.- Section III Protoplast Isolation, Culture and Somatic Hybridization.- III.1 Viability, DNA Synthesis and Cell Wall Regeneration on Potato Protoplasts.- 1. Introduction.- 2. Methodology.- 3. Results and Discussion.- 4. Conclusion.- References.- III.2 Improved Culture Techniques for Potato Protoplasts.- 1. Introduction.- 2. Source of Plant Material.- 3. Protoplast Isolation and Purification.- 4. Protoplast Culture.- 5. Conclusion.- References.- III.3 Regeneration of Plants from Potato Protoplasts.- 1. Introduction.- 2. Pre-Growth of the Cells or Plant.- 3. Production of Protoplasts.- 4. Collection and Washing of Protoplasts.- 5. Culture of Protoplasts and Calli.- 6. Nature of the Protoclones Produced.- References.- III.4 Electrofusion and Analysis of Potato Somatic Hybrids.- 1. Introduction.- 2. Protoplast Preparations.- 3. Electrofusion of Potato Protoplasts.- 4. Characterization of Variants and Fusion Products.- References.- III.5 Markers for Identifying Somatic Hybrids in Potato.- 1. Introduction.- 2. Biochemical Markers.- 3. Transient Differences Between Hybrids and Their Parents...- 4. Gross Phenotypic Differences.- 5. Selectable Genetic Markers.- 6. Conclusion.- References.- Section IV Somaclonal Variation, Selection of Mutants and Resistant Plants.- IV. 1 Effect of the Origin of Explant on Callus Initiation and Differentiation in Potato.- 1. Introduction.- 2. Materials and Methods.- 3. Study of the Aptitudes for Callogenesis and Organogenesis of Various Primary Explants.- 4. Composition of the Callus After Fragmentation and Successive Subculturing.- 5. Histogenesis.- 6. Conclusions.- References.- IV2 In Vitro Induction of Cold Acclimation in Potato.- 1. Introduction.- 2. Potato Cold Acclimation.- 3. The Establishment of Tissue Cultures.- 4. In Vitro Cold Acclimation.- 5. Hormonal Effects on Cold Acclimation.- 6. Plasma Membrane Alterations.- 7. Conclusion.- References.- IV3 Potato Improvement Through In Vitro Selection for Increased Levels of Free Amino Acids.- 1. Introduction.- 2. Selection Methods.- 3. Potato Tissue Culture Selection.- 4. Other Tissue Culture Selections.- 5. Conclusion.- References.- IV.4 Biotechnologies of Obtaining Herbicide Tolerance in Potato.- 1. The Problem and Possible Solutions.- 2. Biotechnological Selection Systems.- 3. Results with Potatoes.- 4. The Future Outlook and Challenges.- References.- IV.5 In Vitro Production of Potatoes Bearing Resistance to Fungal Diseases.- 1. Historic Selection of Potato.- 2. Potato Breeding and Disease Resistance.- 3. Somatic Approach.- 4. Isolation of Phytotoxin.- 5. Resistance Screening of Regenerates.- 6. Regeneration After in Vitro Selection.- 7. Conclusions.- References.- IV.6 Selection of Variants by Dual Culture of Potato and Phytophthora infestans.- 1. Introduction.- 2. Regeneration of Potato Variants.- 3. Dual Culture of Potato and Fungus.- 4. Greenhouse Observations of Variants.- 5. Conclusions and Prospects.- References.- IV.7 Testing Somaclonal Variants of Potato for Resistance to Virus Disease.- 1. Introduction.- 2. Examples of Specific Disease Resistance Studies with Potato.- 3. Previous Work on Resistance to Potato Viruses.- 4. Nature of Resistance to Disease in Somaclones.- 5. Protoplasts Isolation from Potato Leaf Mesophyll.- 6. Other Virus/Viroid Diseases.- 7. Methods for Future Work.- References.- IV.8 Breeding for Virus and Nematode Resistance in Potato Through Microspore Culture.- 1. Introduction.- 2. Methodology.- 3. Results.- 4. Discussion.- References.- IV.9 Genetic Diversity in Protoplast- and Cell-Derived Plants of Potato.- 1. Introduction.- 2. Karyological Observations.- 3. Phenotypic Changes in Protoplast- and Cell-Derived Plants.- 4. Conclusions and Prospects.- References.- IV. 10 Variation in Tubers in Single Cell-Derived Clones of Potato in Ireland.- 1. Introduction.- 2. Single Cell Culture.- 3. Somaclonal Variation in Regenerates from Single Cell Culture.- 4. Discussion.- References.- IV. 11 Genetic Variability in Tuber Disc-Derived Potato Plants.- 1. Introduction.- 2. Tuber Disc Regeneration Procedure.- 3. Origins of Variability.- 4. Variability from Adventitious Regeneration.- 5. Statistical Approach to Evaluation of Quantitative Variation.- 6. Exemplary Analysis of Tuber Elongation Ratio Results.- 7. Conclusions.- References.- IV. 12 In Vitro Induction of Mutation in Potato.- 1. Introduction.- 2. In Vivo Mutation Breeding.- 3. In Vitro Culture and Mutation Breeding.- 4. The Somaclonal Variation.- 5. Discussion and Perspectives.- References.- Section V Conservation and Exchange of Germplasm.- V.1 Preservation of Potato Pollen.- 1. Introduction.- 2. Materials and Methods.- 3. Results and Discussion.- 4. Conclusions.- References.- V.2 In Vitro Conservation of Potato Germplasm in Hungary.- 1. Introduction.- 2. Culture Initiation.- 3. Elimination of Viruses, Bacteria and Fungi.- 4. Maintenance of Cultures.- 5. Maintenance of Viruses in Culture.- 6. Long-Term Storage.- 7. Genetic Stability.- 8. Exchange and Transfer.- 9. Vegetative Propagation.- 10. In Vitro Tuberization.- 11. Planting.- 12. Development of Plants in the Field.- 13. Conclusions.- References.- V.3 Tissue Culture for the International Exchange of Potato and Cassava Germplasm.- 1. Introduction.- 2. Establishment of Gene Banks.- 3. Tissue Culture Methods.- 4. Distribution of Germplasm.- 5. Conclusions.- References.- V.4 Freeze Preservation of Suspension Cultures of Potato.- 1. Introduction.- 2. Preparation of Cell Suspension Culture.- 3. Freeze Preservation.- References.- V.5 Cryopreservation of Potato Germplasm.- 1. Introduction.- 2. Cryopreservation of Excised Meristems.- 3. Cryopreservation of Root Tips.- 4. Cryopreservation of Cell Suspensions and Somaclones.- 5. Cryopreservation of Pollen.- 6. Viability and Stability During Storage.- 7. Clonal Repositories and the International Exchange of Germplasm.- 8. Conclusions and Prospects.- References.- V.6 Fluid Drilling of Embryos in Potato Improvement - A Future Possibility.- 1. Problems of Potato 'Seed' Production.- 2. Alternative Planting Systems.- 3. Somatic Embryogenesis in Herbaceous Crops.- 4. Delivery Systems for Somatic Embryos.- 5. Recent Developments in Potato Tissue Culture.- 6. Future of Fluid Drilling of Potato Somatic Embryos.- References.

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