Overview

This book details the synthesis and assembly of polypeptide materials across length scales, i.e. proteins and peptides, their precursors, conjugates, and derivatives. A particular emphasis is made on measurement tools and procedures for material characterization, both physicochemical and functional. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and practical, Polypeptide Materials: Methods and Protocols serves to reflect the inter-disciplinary nature of molecular biology as well as the importance of developing innovative measurement methods to advance this vital area of research.

Full Product Details

Author:   Maxim G. Ryadnov
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2021
Volume:   2208
Weight:   0.544kg
ISBN:  

9781071609309

ISBN 10:   1071609300
Pages:   268
Publication Date:   28 August 2021
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

A Shortcut to the Synthesis of Peptide Thioesters Using an HF-Free Approach.- SnoopLigase-Mediated Peptide-Peptide Conjugation and Purification.- Peptide Nanoparticles for Gene Packaging and Intracellular Delivery.- Synthesis and Application of Peptide-siRNA Nanoparticles from Disulfide-Constrained Cyclic Amphipathic Peptides for the Functional Delivery of Therapeutic Oligonucleotides to the Lung.- FRET-Mediated Observation of Protein-Triggered Conformational Changes in DNA Nanostructures.- Molecular Simulations Guidelines for Biological Nanomaterials: From Peptides to Membranes.- Functional Peptide Nanocapsules Self-Assembled from b-Annulus Peptides.- Electrostatic Self-Assembly of Protein Cage Arrays.- Design and Generation of Self-Assembling Peptide Virus-Like Particles with Intrinsic GPCR Inhibitory Activity.- Imaging and 3D Reconstruction of De Novo Peptide Capsids.- Monitoring the Assembly and Aggregation of Polypeptide Materials by Time-Resolved Emission Spectra.- Forming Low Molecular Weight Hydrogels by Electrochemical Methods.- In Situ Measurements of Polypeptide Samples by Dynamic Light Scattering: Membrane Proteins, a Case Study.- Measurement of Peptide Coating Thickness and Chemical Composition Using XPS.- Imaging the Effects of Peptide Materials on Phospholipid Membranes by Atomic Force Microscopy.- Microfluidic Single-Cell Phenotyping of the Activity of Peptide-Based Antimicrobials.- Ultramicrotomy Analysis of Peptide-Treated Cells.

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