Overview
This detailed volume explores poly(ADP-ribose) polymerases (PARPs) in the biology of eukaryotes and their relevance to human health. Beginning with a section on the detection and quantification of poly(ADP-ribose) polymer (pADPr), the book continues by delving into the identification of protein targets, functional analysis, the poly(ADP-ribosyl)ating pathway in chromatin and genes expression, as well as the use of animal models and PARP1 inhibitor design and testing, and more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Poly(ADP-Ribose) Polymerase: Methods and Protocols, Third Edition presents essential new and classical methods for studying the pADPr-pathway.
Full Product Details
Author: Alexei V. Tulin
Publisher: Springer-Verlag New York Inc.
Imprint: Springer-Verlag New York Inc.
Edition: 3rd ed. 2023
Volume: 2609
Weight: 1.063kg
ISBN: 9781071628904
ISBN 10: 1071628909
Pages: 446
Publication Date: 15 December 2022
Audience:
Professional and scholarly
,
Professional & Vocational
Format: Hardback
Publisher's Status: Active
Availability: Manufactured on demand 
We will order this item for you from a manufactured on demand supplier.
Table of Contents
Part I: Detection and Quantification of pADPr 1. Fluorescence-Based Analyses of Poly(ADP-Ribose) Length by Gel Electrophoresis, High Performance Liquid Chromatography, and Capillary Electrophoresis Mohsen Badiee, Audrey Boutonnet, Dat Phan, and Anthony K.L. Leung 2. Detecting and Quantifying pADPr In Vivo Andrew M. Lamade, Yaming Chen, Carla J. Johnson, Hülya Bayır, and Robert S.B. Clark 3. Quantitative Analysis of Nuclear Poly(ADP-Ribose) Dynamics in Response to Laser-Induced DNA Damage Christopher A. Koczor, Kate M. Saville, Rasha Q. Al-Rahahleh, Joel F. Andrews, Jianfeng Li, and Robert W. Sobol 4. Analyzing PARP1 Activity: Small Molecule Reactants and Attached Chains of Poly(ADP-Ribose) Johannes Rudolph and Karolin Luger 5. Detecting Poly(ADP-Ribose) In Vitro and in Cells Using PAR-Trackers Sridevi Challa, Amy L. Whitaker, and W. Lee Kraus 6. An Enzyme-Linked Immunosorbent Assay to Quantify Poly(ADP-Ribose) Level In Vivo Chieri Ida, Sachiko Yamashita, Takayuki Eguchi, Yasuhito Kuroda, Setsu Nakae, Yoshisuke Nishi, Kazuo Kamemura, Tsuyoshi Shirai, Tamio Mizukami, Masakazu Tanaka, Joel Moss, and Masanao Miwa 7. Subcellular Quantitation of ADP-Ribosylation by High Content Microscopy Lukas Muskalla, Anka Güldenpfennig, and Michael O. Hottiger 8. A Simple Method to Study ADP-Ribosylation Reversal: From Function to Drug Discovery Johannes Gregor Matthias Rack and Ivan Ahel Part II: Identification of Protein Targets 9. Immunoprecipitation Using Mono-ADP-Ribosylation Specific Antibodies Helen Dauben and Ivan Matic 10. A Clickable NAD+ Analog-Based Assay of Poly(ADP-Ribosyl)ated Proteins Dongsheng Yao, Heba Ahmed, and Junqi Song 11. Functional Analysis of Histone ADP-Ribosylation In Vitro and in Cells Dan Huang, Andrea D. Edwards, Xuan Gong, and W. Lee Kraus Part III: Functional Analysis 12. Studying the Immunomodulatory Functions of PARP1 and PARP2 in Mouse Models of Cancer Nura Lutfi, Carlos Martínez, and José Yélamos 13. Methods for Investigating Transient Receptor Potential Melastatin-2 (TRPM2), a Cation Channel Activated by ADP-Ribose and Involved in Cell Death Hannah K. Hall and David W. Koh 14. Methods to Assess the Role of PARPs in Regulating Mitochondrial Oxidative Function Tünde Kovács, Boglárka Rauch, Edit Mikó, and Péter Bai 15. Characterizing ADP-Ribosylation Sites Using Af1521 Enrichment Coupled to ETD-Based Mass Spectrometry Holda A. Anagho, Jonas D. Elsborg, Ivo A. Hendriks, Sara C. Buch-Larsen, and Michael L. Nielsen 16. Cytological Approaches to Visualize Intracellular Dynamics of RNA Binding Proteins at Active Genes in Drosophila Anand K. Singh and Subhash C. Lakhotia Part IV: Poly(ADP-Ribosyl)ating Pathway in Chromatin and Gene Expression 17. Chromatin Immunoprecipitation Approach to Determine How PARP1 Domains Affect Binding Pattern to Chromatin Guillaume Bordet, Gbolahan Bamgbose, Sayem H. Bhuiyam, Sarah Johnson, and Alexei V. Tulin 18. Approach to Measuring the Effect of PARP1 on RNA Polymerase II Elongation Rates Hejer Dhahri, Elena Matveeva, and Yvonne Fondufe-Mittendorf 19. Examining the Effect of PARP-1 Inhibitors on Transcriptional Activity of Androgen Receptor in Prostate Cancer Cells Peter Makhov, Rushaniya Fazliyeva, Antonio Tufano, Robert G. Uzzo, and Vladimir M. Kolenko Part V: Using Animal Models 20. Using Drosophila Genetics to Identify Factors that Affect PARP1 Activity In Vivo Guillaume Bordet and Alexei V. Tulin 21. Generating PARP Knockout D. Melanogaster with CRISPR/Cas9 System Yaroslava Karpova and Alexei V. Tulin 22. TaqMan Multiplex qPCR Method to Genotype PARG Knockout Mice Yaroslava Karpova and Alexei V. Tulin Part VI: Designing and Testing PARP-1 Inhibitors 23. Cell-Based Screening for New PARP Inhibitors Utilizing PARG Mutated Mouse Embryonic Stem Cells Yaroslava Karpova, Danping Guo, and Alexei V. Tulin 24. Quantification of PARP7 Protein Levels and PARP7 Inhibitor Target Engagement in Cells Using a Split Nanoluciferase System Anna K. Duell, Daniel J. Sanderson, and Michael S. Cohen Part VII: Targeting the Understudied Components of pADPr Pathway 25. Purification of Recombinant Human PARG and Activity Assays Jean-Christophe Amé and Françoise Dantzer 26. Purification of Recombinant Human PARP-3 Jean-Christophe Amé, Leonel Nguekeu-Zebase, Daisy Harwood, Zuleyha Yildirim, Lisa Roegel, Agathe Boos, and Françoise Dantzer
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