Overview

This volume provides methodologies for ES and iPS cell technology on the study of cardiovascular diseases. Chapters guide readers through protocols on cardiomyocyte generation from pluripotent stem cells, physiological measurements, bioinformatic analysis, gene editing technology, and cell transplantation studies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Authoritative and cutting-edge, Pluripotent Stem-Cell Derived Cardiomyocytes aims to help researchers set up experiments using pluripotent stem cell-derived cardiac cells.

Full Product Details

Author:   Yoshinori Yoshida
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2021
Volume:   2320
Weight:   0.610kg
ISBN:  

9781071614860

ISBN 10:   107161486
Pages:   304
Publication Date:   26 July 2022
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Part I: Overview 1. Making Cardiomyocytes from Pluripotent Stem Cells       Peter Karaginius and Yoshinori Yoshida   Part II: Generation of Pluripotent Stem Cell-Derived Cardiomyocytes and Cardiac Tissues 2. A method for Large-Scale Cardiac Differentiation, Purification, and Cardiac Spheroid Production of Human Induced Pluripotent Stem Cells          Yuika Morita, Shugo Tohyama, Jun Fujita, and Keiichi Fukuda   3. Large-scale Differentiation of Stem Cell-Derived Cardiomyocytes by Stirring-Type Suspension Culture           Nagako Sougawa, Shigeru Miyagawa, and Yoshiki Sawa   4. Efficient Method to Dissociate Induced Pluripotent Stem Cell-derived Cardiomyocyte Aggregates into Single Cells    Emiko Ito, Shigeru Miyagawa, Yoshinori Yoshida, and Yoshiki Sawa   5. Isolation of Cardiomyocytes Derived from Human Pluripotent Stem Cells using miRNA switches        Kenji Miki, Hirohide Saito, and Yoshinori Yoshida   6. Fabrication of Cardiac Constructs using bio-3D Printer    Kenichi Arai, Daiki Murata, Shoko Takao, and Koichi Nakayama   7. Fabrication of Thick and Anisotropic Cardiac Tissue on Nanofibrous Substrate for Repairing Infarcted Myocardium    Junjun Li, Li Liu, Itsunari Minami, Shigeru Miyagawa, and Yoshiki Sawa   8. Construction of Three-Dimensional Cardiac Tissues Using Layer by Layer Method        Maki Takeda, Shigeru Miyagawa, Mitsuru Akashi, and Yoshiki Sawa   9. Generation of Cylindrical Engineered Cardiac Tissues from human iPS Cell-derived Cardiovascular Cell Lineages            Hidetoshi Masumoto   Part III: Physiological Mesurements using Pluripotent Stem Cell-Derived Cardiomyocytes   10. Protocol for Morphological and Functional Phenotype Analysis of hiPS-derived Cardiomyocytes       Jun LI and Jong-Kook LEE   11. Application of FluoVolt Membrane Potential Dye for Induced Pluripotent Stem Cell-derived Cardiac Single Cells and Monolayers Differentiated via Embryoid Bodies  Tadashi Takaki and Yoshinori Yoshida   12. Multi-Electrode Array Assays Using Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes            Daisuke Yoshinaga, Yimin Wuriyanghai, and Takeru Makiyama   13. Electrophysiological Analysis of hiPSC-derived Cardiomyocytes Using a Patch-Clamp Technique      Yuta Yamamoto, Sayako Hirose, Yimin Wuriyanghai, Daisuke Yoshinaga, and Takeru Makiyama   14. Characterization of Ventricular and Atrial Cardiomyocyte Subtypes from Human Induced Pluripotent Stem Cells            Misato Koakutsu, Tadashi Takaki, Kenji Miki, and Yoshinori Yoshida   15. Assessment of Contractility in Human iPS Cell-Derived Cardiomyocytes Using Motion Vector Analysis        Yasunari Kanda, Ayano Satsuka, Sayo Hayashi, Mihoko Hagiwara-Nagasawa, and Atsushi Sugiyama   16. Contractile Force Measurement of Engineered Cardiac Tissues Derived from Human iPS Cells Daisuke Sasaki, Katsuhisa Matsuura, and Tatsuya Shimizu   17. A Method for Contraction Force Measurement of iPSC Derived Engineered Cardiac Tissues Yuya Fujiwara, Kohei Deguchi, Kenji Miki, Tomoyuki Nishimoto, and Yoshinori Yoshida   Part IV: Transcriptome and Bioinfomatics Analysis 18. Single-cardiomyocyte RNA Sequencing to Dissect the Molecular Pathophysiology of the Heart Manami Kotoh, Seitaro Nomura, Shintaro Yamada,Hiroyuki Aburatani, and Issei Komuro   19. RNA-sequencing Analysis of Differentially Expressed Genes in Human iPSC-derived Cardiomyocytes         Chikako Okubo, Megumi Narita, Takuya Yamamoto, and Yoshinori Yoshida   20. Analysis of Transcriptional Profiling of Chamber-specific Human Cardiac Myocytes Derived from Pluripotent Stem Cells Antonio Lucena-Cacace and Yoshinori Yoshida   Part V: Gene Editing and CRISPR Technology for Pluripotent Stem Cells 21. Genome Editing in Human Induced Pluripotent Stem Cells (hiPSCs)    Shuichiro Higo, Shungo Hikoso, Shigeru Miyagawa, and Yasushi Sakata   22. Generation of Efficient Knock-in Mouse and Human Pluripotent Stem Cells Using CRISPR-Cas9     Tatsuya Anzai, Hiromasa Hara, Chanthra Nawin, Taketaro Sadahiro, Masaki Ieda, Yutaka Hanazono, and Hideki Uosaki   23. CRISPRi/a Screening with Human iPSCs            Masataka Nishiga, Lei S. Qi, and Joseph C. Wu   Part VI: Transplantation of Pluripotent Stem Cell-Derived Cardiomyocytes 24. Transplantation of Human Induced Pluripotent Stem Cell-derived Cardiomyocytes in a Mouse Myocardial Infarction Model         Takeshi Hatani and Yoshinori Yoshida   25. Transplantation of Pluripotent Stem Cell-derived Cardiomyocytes into a Myocardial Infarction Model of Cynomolgus Monkey  Hideki Kobayashi, Hajime Ichimura, Noburou Ohashi, and Yuji Shiba

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