Overview

This fully updated book aims to facilitate the study of the nanochannels that connect plant cells, known as plasmodesmata, and to instigate new research that will further advance our knowledge of these structures. Beginning with the general structural composition and regulation of plasmodesmata as well as their role in plant development and disease, the volume continues with chapters exploring plasmodesmata architectures and distribution in cell interfaces, approaches to dissect plasmodesmata composition, protocols to quantify changes in plasmodesmata permeability using fluorescent tracers and mobile proteins, as well as a section with protocols that contribute to plasmodesmata research but fall outside the previous classifications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and up-to-date, Plasmodesmata: Methods and Protocols, Second Edition serves as a vital guide for all plant scientists, both novice and expert, especially those studying the intricacies of cell-to-cell communication pathways. 

Full Product Details

Author:   Yoselin Benitez-Alfonso ,  Manfred Heinlein
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   2nd ed. 2022
Volume:   2457
Weight:   1.099kg
ISBN:  

9781071621318

ISBN 10:   1071621319
Pages:   470
Publication Date:   30 March 2022
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Part I: Overview Articles   1. Plasmodesmata Structural Components and Their Role in Signaling and Plant Development             Philip Kirk and Yoselin Benitez-Alfonso   2. Function of Plasmodesmata in the Interaction of Plants with Microbes and Viruses             Caiping Huang and Manfred Heinlein   Part II: Structure and Distribution of Plasmodesmata   3. Plasmodesmata Ultrastructure Determination Using Electron Tomography             Jules D. Petit, Marie Glavier, Lysiane Brocard, and Emmanuelle Bayer   4. Ultrastructural Analysis and Three-Dimensional Reconstruction of Plasmodesmata             Kamila Godel-Jędrychowska, Tilman Franke, and Ewa Kurczyńska   5. Serial Block Electron Microscopy to Study Plasmodesmata in the Vasculature of Arabidopsis thaliana Roots             Andrea Paterlini and Ilya Belevich   6. Focused Ion Beam-Scanning Electron Microscopy for Investigating Plasmodesmal Densities             Brandon C. Reagan, John R. Dunlap, and Tessa M. Burch-Smith   7. Measuring Plasmodesmata Density on Cell Interfaces of Monocot Leaves Using 3D Immunolocalization and Scanning Electron Microscopy             Florence R. Danila   8. Super-Resolution Imaging of Plasmodesmata Using 3D-Structured Illumination Microscopy             Kirsten Knox   Part III: Compositional Analysis of Plasmodesmata   9. In Vivo Aniline Blue Staining and Semi-Automated Quantification of Callose Deposition at Plasmodesmata             Caiping Huang, Jerôme Mutterer, and Manfred Heinlein   10. Immunofluorescence Detection of Callose in Plant Tissue Sections             Sam Amsbury and Yoselin Benitez-Alfonso   11. Callose Detection and Quantification at Plasmodesmata in Bryophytes             Arthur Muller, Tomomichi Fujita, and Yoan Coudert   12. Isolation of Plasmodesmata Membranes for Lipidomic and Proteomic Analysis             Laetitia Fouillen, Stéphane Claverol, Emmanuelle M.F. Bayer, and Magali S. Grison   13. Methods for Detection of Protein Interactions with Plasmodesmata-Localized Reticulons             Verena Kriechbaumer and Stanley W. Botchway   14. Studying Protein-Protein Interactions at Plasmodesmata by Measuring Förster Resonance Energy Transfer             Patrick Blümke, Vicky Howe, and Rüdiger Simon   15. Quantifying the Organization and Dynamics of the Plant Plasma-Membrane across Scales Using Light Microscopy             Joseph F. McKenna   16. Using Steady-State Fluorescence Anisotropy to Study Protein Clustering             Ajeet Chaudhary and Kay Schneitz   Part IV: Functional Analysis of Plasmodesmata   17. Quantification of Cell-to-Cell Connectivity Using Particle Bombardment             Estee E. Tee, Sebastian Samwald, and Christine Faulkner   18. Investigating Plasmodesmata Function in Arabidopsis thaliana Using a Low-Pressure Bombardment System and GFP Movement Assay             Jessica C. Fernandez and Tessa M. Burch-Smith   19. Quantifying Plasmodesmatal Transport with an Improved GFP Movement Assay             Wilson Horner and Jacob O. Brunkard   20. An Arabidopsis Callus Grafting Method to Test Cell-to-Cell Mobility of Proteins             Frank Machin, Yağmur Hasbioğlu, and Friedrich Kragler   21. Quantification of Plasmodesmata Permeability in Arabidopsis Leaves by Tracing the Movement of GFP             Min Diao and Shanjin Huang   22. Tracking Intercellular Movement of Fluorescent Proteins in Bryophytes             Takumi Tomoi, Yoan Coudert, and Tomomichi Fujita   23. Virus Genome-Based Reporter for Analyzing Viral Movement Proteins and Plasmodesmata Permeability             Ekaterina A. Lazareva, Anastasia K. Atabekova, Alexander A. Lezzhov, Sergey Y. Morozov, Manfred Heinlein, and Andrey G. Solovyev   24. Analysis of the Distribution of Symplasmic Tracers during Zygotic and Somatic Embryogenesis             Justyna Wróbel-Marek, Kamila Godel-Jędrychowska, and Ewa Kurczyńska   25. Quantifying Intercellular Movement and Protein Stoichiometry for Computational Modeling             Lisa Van den Broeck, Mariah Gobble, and Rosangela Sozzani   26. Spatiotemporal Specific Blocking of Plasmodesmata by Callose Induction             Dawei Yan   27. A Forward Genetic Approach to Identify Plasmodesmal Trafficking Regulators Based on Trichome Rescue             Munenori Kitagawa and David Jackson   Part V: Other Approaches Contributing to Plasmodesmata Research   28. In Vivo Visualization of Mobile mRNA Particles in Plants Using BglG             Eduardo J. Peña and Manfred Heinlein   29. Multi-Angle In Vivo Imaging of the Arabidopsis thaliana Shoot Apical Meristem (SAM)             Michael Fuchs and Jan U. Lohmann   30. More Insights from Ultrastructural and Functional Plasmodesmata Data Using PDinsight             Eva E. Deinum   31. Measuring Intercellular Interface Area in Plant Tissues Using Quantitative 3D Image Analysis             Gwendolyn V. Davis, Richard S. Smith, and George W. Bassel

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