Overview

This manual comprises a broad range of techniques of value to research workers in the fields of cell and molecular biology, physiology, plant breeding and propagation, and genetic engineering. Both applied and more fundamental uses of cell, tissue and organ culture are covered, and the ring-binder design allows the manual to be updated regularly with supplementary chapters. Each chapter, discussing a single technique or protocol, is written by an authority in the field and provides background information, references to the relevant literature and a guide to troubleshooting in addition to a thorough methodology.

Full Product Details

Author:   K. Lindsey
Publisher:   Springer
Imprint:   Springer
Edition:   1995 ed.
Weight:   0.275kg
ISBN:  

9780792333197

ISBN 10:   0792333195
Pages:   164
Publication Date:   21 July 1995
Audience:   College/higher education ,  Professional and scholarly ,  Postgraduate, Research & Scholarly ,  Professional & Vocational
Format:   Book
Publisher's Status:   Active
Availability:   Out of stock  
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Table of Contents

Section A Basic Techniques: Cell and Tissue Culture of Model Species. 1. Media preparation; O.L. Gamborg. 2. The initiation and maintenance of callus cultures of carrot and tobacco; R.D. Hall. 3. The initiation and maintenance of plant cell suspension cultures; R.D. Hall. 4. Shoot cultures and root cultures of tobacco; J.F. Topping, K. Lindsey. 5. Somatic embryogenesis in orchardgrass; M.E. Horn. 6. Arabidopsis regeneration and treasformation (leaf and cotyledon explant system); R. Schmidt, L. Willmitzer. 7. Arabidopsis protoplast transformation and regeneration; B. Damm, L. Willmitzer. 8. Arabidopsis regeneration and transformation (root explant system); D. Valvekens, M. van Lijsebettens, M. van Montagu. 9. Somatic embryogenesis in carrot; A.D. Krikorian, D.L. Smith. 10. Low density cultures: microdroplets and single-cell nurse cultures; G. Spangenberg, H.-U. Koop. 11. Tobacco protoplast isolation, culture and regeneration; I. Negrutiu. Section B Tissue Culture and Transformation of Crop Species. 1. Embryogenic callus, cell suspension and protoplast cultures of cereals; I.K. Vasil, V. Vasil. 2. Transformation and regeneration of rice protoplasts; J. Kyozuka, K. Shimamoto. 3. Transformation and regeneration of orchardgrass protoplasts; M.E. Horn. 4. Transformation and regeneration of oilseed rape protoplasts; D. Rouan, P. Guerche. 5. Regeneration and transformation of potato by Agrobacterium tumefaciens; R.G.F. Visser. 6. Transformation of tomato with Agrobacterium tumefaciens; S. McCormick. 7. Regeneration and transformation of sugarbeet by Agrobacterium tumefaciens; K. Lindsey, P. Gallois, C. Eady. 8. Regeneration and transformation of apple (Malus pumila Mill.); D.J. James, A.M. Dandekar. 9. Transformation and regeneration of maize protoplasts; C.A. Rhodes, D.W. Gray. 10. Regeneration and transformation of barley protoplasts; P. Lazzeri, A. Jahne, H. Lorz. 11. Agrobacterium-mediated transformation of potato stem and tuber tissue, regeneration and PCR screening for transformation; J.P. Spychalla, M.W. Bevan. Section C Propagation and Conservation of Germplasm. 1. Clonal propagation of orchids; Y. Sagawa. 2. Clonal propagation of palms; B. Tisserat. 3. Clonal propagation of conifers; T.A. Thorpe, I.S. Harry. 4. Cytological techniques; A. Karp. 5. Restriction fragment analysis of somaclones; R.H. Potter. 6. Virus elimination and testing; M.C. Coleman, W. Powell. 7. Clonal propagation of Citrus; T.S. Rangan. 8. Clonal propagation of Eucalypts; J.A. McComb. Section D Direct Gene Transfer and Protoplast Fusion. 1. Gene transfer by particle bombardment; T.M. Klein, S. Knowlton, R. Arentzen. 2. Transformation of pollen by particle bombardment; D. Twell, T.M. Klein, S. McCormick. 3. Electrical fusion of protoplasts; M.G.K. Jones. 4. Cybrid production and selection; E. Galun, D. Aviv. (Part contents).

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