Overview

This detailed volume presents a wide range of techniques for plant mitochondrial analysis, ranging from tried-and-tested work horse techniques to the latest innovations. Within its pages, it explores subjects such as affinity-based isolation of mitochondria with magnetic beads, mitochondrial quality assessment protocols, measurement of uptake and release of specific metabolites, mitochondrial protein identification and visualization, as well as gene splicing and editing, and much more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and practical, Plant Mitochondria: Methods and Protocols provides a highly useful set of methodologies for the plant mitochondrial community to help discover more interesting aspects of plant mitochondria in the years to come.

Full Product Details

Author:   Olivier Van Aken ,  Allan G. Rasmusson
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2022
Volume:   2363
Weight:   0.677kg
ISBN:  

9781071616550

ISBN 10:   1071616552
Pages:   342
Publication Date:   25 September 2021
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

1. Purification of Leaf Mitochondria from Arabidopsis thaliana Using Percoll Density Gradients             Huy Cuong Tran and Olivier Van Aken   2. Cell Type-Specific Isolation of Mitochondria in Arabidopsis             Clément Boussardon and Olivier Keech   3. Isolation of Plant Mitochondria Using Affinity Purification             Franziska Kuhnert and Andreas P.M. Weber   4. Isolation of Highly Purified, Intact, and Functional Mitochondria from Potato Tubers Using a Two-in-One Percoll Density Gradient             Ian Max Møller and Allan G. Rasmusson   5. Integrity Assessment of Isolated Plant Mitochondria             Allan G. Rasmusson, Mengshu Hao, and Ian Max Møller   6. High-Throughput Oxygen Consumption Measurements in Leaf Tissue Using Oxygen Sensitive Fluorophores             Brendan M. O'Leary, Glenda Guek Khim Oh, and A. Harvey Millar   7. Assessment of Respiratory Enzymes in Intact Cells by Permeabilization with Alamethicin Allan G. Rasmusson, Ian Max Møller, and Susanne Widell   8. Assessing the Kinetics of Metabolite Uptake and Utilization by Isolated Mitochondria Using Selective Reaction Monitoring-Mass Spectrometry (SRM-MS)             Xuyen Le, A. Harvey Millar, and Chun Pong Lee   9. Complexome Profiling of Plant Mitochondrial Fractions             Lucie Schröder, Holger Eubel, and Hans-Peter Braun   10. High Throughput BN-PAGE for Mitochondrial Respiratory Complexes             Lilian Vincis Pereira Sanglard and Catherine Colas des Francs-Small   11. Assessment of Mitochondrial Protein Composition and Purity by Mass Spectroscopy             Anqiang Tang, Yimin Wang, and Nicolas L. Taylor   12. Mitochondrial GFP-Tagged Protein Localization Using Transient Transformations in Arabidopsis thaliana             Cristina Ruberti   13. Assessment of Mitochondrial Protein Topology and Membrane Insertion             Kerstin Schäfer, Carina Engstler, Korbinian Dischinger, and Chris Carrie   14. Assessment of Protein Synthesis in Mitochondria Isolated from Rosette Leaves and Liquid Culture Seedlings of Arabidopsis             Malgorzata Kwasniak-Owczarek, Artur Tomal, and Hanna Janska   15. Markers for Mitochondrial ROS Status             Monika Ostaszewska-Bugajska, Anna Podgórska, and Bożena Szal   16. Mass Spectrometry-Based Quantitative Cysteine Redox Proteome Profiling of Isolated Mitochondria Using Differential iodoTMT Labeling             Jonas Giese, Jürgen Eirich, Frederik Post, Markus Schwarzländer, and Iris Finkemeier   17. Analysis of the Plant Mitochondrial Transcriptome             Kevin Baudry, Etienne Delannoy, and Catherine Colas des Francs-Small   18. Quantification of Mitochondrial RNA Editing Efficiency Using Sanger Sequencing Data             Mizuki Takenaka   19. Nascent Transcript Sequencing for the Mapping of Promoters in Arabidopsis thaliana Mitochondria             Omar Saleh, Sarlita Dwiani, Julia Rott, and Kristina Kühn   20. Assessment of Mitochondrial DNA Copy Number, Stability, and Repair in Arabidopsis             Déborah Schatz-Daas, Arnaud Fertet, Frédérique Lotfi, and José M. Gualberto   21. Measuring the Activity of DNA Repair Enzymes in Isolated Mitochondria             Beatriz Ferrando, Ian Max Møller, and Tinna Stevnsner   22. MitoTALENs: A Method for Targeted Gene Disruption in Plant Mitochondrial Genomes             Shin-ichi Arimura

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