Overview

This detailed volume provides a collection of protocols for the study of miRNA functions in plants. Beginning with coverage of miRNA function, biogenesis, activity, and evolution in plants, the book continues by guiding readers through methods on the identification and detection of plant miRNAs, bioinformatic analyses, and strategies for functional analyses of miRNAs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and cutting-edge, Plant MicroRNAs: Method and Protocols aims to ensure successful results in the further study of this vital area of plant science.

Full Product Details

Author:   Stefan de Folter
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   1st ed. 2019
Volume:   1932
Weight:   0.928kg
ISBN:  

9781493990412

ISBN 10:   1493990411
Pages:   363
Publication Date:   31 January 2019
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

1. An Introduction to Methods for Discovery and Functional Analysis of MicroRNAs in Plants Alma Armenta-Medina and C. Stewart Gillmor 2. The Plant MicroRNA Pathway: The Production and Action Stages Joseph L. Pegler, Christopher P. Grof, and Andrew L. Eamens 3. Plant miRNA Conservation and Evolution Lydia Gramzow and Gunter Theissen 4. Bioinformatic Analysis of Small RNA Sequencing Libraries Ricardo A. Chavez Montes, Fabiola Jaimes Miranda, and Stefan de Folter 5. An Overview of miRNA and miRNA Target Analysis Tools Anna Lukasik and Piotr Zielenkiewicz 6. miRLocator: A Python Implementation and Web Server for Predicting miRNAs from Pre-miRNA Sequences Ting Zhang, Lie Ju, Jingjing Zhai, Yujia Song, Jie Song, and Chuang Ma 7. Prediction of Plant miRNA Targets Priyanka Pandey, Prashant K. Srivastava, and Shree P. Pandey 8. Isolation and Detection Methods of Plant miRNAs Pedro Fernando Vera Hernandez, Stefan de Folter, and Flor de Fatima Rosas Cardenas 9. Northern Blot Analysis of MicroRNAs and Other Small RNAs in Plants Carlos De la Rosa and Jose Luis Reyes 10. miRNA Detection by Stem-Loop RT-qPCR in Studying MicroRNA Biogenesis and MicroRNA Responsiveness to Abiotic Stresses Aleksandra Smoczynska, Pawel Sega, Agata Stepien, Katarzyna Knop, Artur Jarmolowski, Andrzej Pacak, and Zofia Szweykowska-Kulinska 11. Detection of miRNAs by Tissue Printing and Dot Blot Hybridization Marcelino Martinez Nunez, Stefan de Folter, and Flor de Fatima Rosas Cardenas 12. In Situ Localization of Small RNAs in Plants Cristina F. Marco, Damianos S. Skopelitis, and Marja C.P. Timmermans 13. Expression Pattern of Plant miRNAs by Classical Transcriptional Fusion Constructs Andrea Tovar-Aguilar, Karla A. Sanchez-Elizondo, Alejandra Rodriguez-Rodriguez, Marcos I. Gonzalez-Jaime, Genaro Patino-Lopez, Vadim Perez-Koldenkova, Jesus A. Badillo-Corona, and Noe Duran-Figueroa 14. A Simple Protocol for Imaging Floral Tissues of Arabidopsis with Confocal Microscopy Andrea Gomez-Felipe and Stefan de Folter 15. Degradome Sequencing in Plants Shih-Shun Lin, Yihua Chen, and Mei-Yeh Jade Lu 16. Functional Analysis of Root MicroRNAs by a Constitutive Overexpression Approach in a Composite Plant System Damien Formey, Jose Angel Martin-Rodriguez, and Georgina Hernandez 17. The Use of MicroRNA Decoy Technologies to Inhibit miRNA Function in Arabidopsis Gigi Wong and Anthony A. Millar 18. Mimicry Technology: A Versatile Tool for Small RNA Suppression Luis Manuel Villar-Martin and Ignacio Rubio-Somoza 19. Design and High-Throughput Generation of Artificial Small RNA Constructs for Plants Alberto Carbonell 20. Detection of MicroRNA Processing Intermediates through RNA Ligation Approaches Belen Moro, Arantxa M.L Rojas, and Javier F. Palatnik 21. Analysis of Methylation Status of Plant MicroRNAs Susu Chen and Guodong Ren 22. Detection of Slicer Activity by Immunopurified Plant ARGONAUTE1 Laura Arribas-Hernandez, Maria Louisa Vigh, and Peter Brodersen 23. The Isolation of Total and Membrane-Bound Polysomes from Arabidopsis and the Detection of Their Associated AGO1 and sRNAs Shengben Li 24. Immunolocalization to Study ARGONAUTE Proteins in Developing Ovules of the Brassicaceae Gloria Leon-Martinez, Edgar Demesa-Arevalo, and Jean-Philippe Vielle-Calzada 25. Detection of Histone Modifications Associated with miRNAs Sara Hernandez-Castellano and Clelia De-la-Pena

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