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Author:   Teun Munnik ,  Ingo Heilmann
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   Softcover reprint of the original 1st ed. 2013
Volume:   1009
Dimensions:   Width: 17.80cm , Height: 1.70cm , Length: 25.40cm
Weight:   6.033kg
ISBN:  

9781493959273

ISBN 10:   1493959271
Pages:   305
Publication Date:   23 August 2016
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Analyzing Plant Signaling Phospholipids through 32Pi-Labeling and TLC.- Analysis of D3-,4-,5-phosphorylated Phosphoinositides using HPLC.- Mass Measurement of Polyphosphoinositides by Thin Layer- and Gas Chromatography.- Measurement of Inositol (1,4,5) Trisphosphate in Plant Tissues by a Competitive Receptor Binding Assay.- Quantification of Diacylglycerol by Mass Spectrometry.- Distinguishing Phosphatidic Acid Pools from de novo Synthesis, PLD and DGK.- Use of Phospholipase A2 for the Production of Lysophospholipids.- Analysis and Quantification of Plant Membrane Lipids by Thin-Layer Chromatography and Gas Chromatography.- Lipidomic Analysis of Plant Membrane Lipids by Direct Infusion Tandem Mass Spectrometry.- Detection and Quantification of Plant Sphingolipids by LC-MS.- Analysis of Defense Signals in Arabidopsis thaliana Leaves by Ultra Performance Liquid Chromatography/ Tandem Mass Spectrometry: Jasmonates, Salicylic Acid, Abscisic Acid.- Analysis of Fatty Acid Amide Hydrolase (FAAH) Activity in Plants.- Ionization Behavior of Polyphosphoinositides Determined via the Preparation of pH Titration Curves using Solid State 31P NMR.- Phosphatidylinositol Synthase Activity from Plant Membrane Fractions and from E. coli-Expressed Recombinant Peptides.- Phosphatidylinositol 4-kinase and Phosphatidylinositol 4-phosphate 5-kinase Assays.- Assaying Inositol and Phosphoinositide Phosphatase Enzymes.- Determination of Phospholipase C Activity In Vitro.- Assaying Non-Specific Phospholipase C (NPC) Activity.- Assaying Different Types of Plant Phospholipase D Activities In Vitro.- Measuring PLD Activity In Vivo.- Assaying Plant Phosphatidic Acid Phosphatase Activity.- Assay of Phospholipase A Activity.- Lipid-Binding Analysis using a Fat Blot Assay.- Liposome-binding Assays to Assess Specificity and Affinity of Phospholipid-Protein Interactions.- Lipid affinity beads – FromIdentifying New Lipid Binding Proteins to Assessing their Binding Properties.- Using Genetically Encoded Fluorescent Reporters to Image Lipid Signaling in Living Plants.- Imaging Changes in Cytoplasmic Calcium using the Yellow Cameleon 3.6 Biosensor and Confocal Microscopy.- kinase Assays.- Assaying Inositol and Phosphoinositide Phosphatase Enzymes.- Determination of Phospholipase C Activity In Vitro.- Assaying Non-Specific Phospholipase C (NPC) Activity.- Assaying Different Types of Plant Phospholipase D Activities In Vitro.- Measuring PLD Activity In Vivo.- Assaying Plant Phosphatidic Acid Phosphatase Activity.- Assay of Phospholipase A Activity.- Lipid-Binding Analysis using a Fat Blot Assay.- Liposome-binding Assays to Assess Specificity and Affinity of Phospholipid-Protein Interactions.- Lipid affinity beads – From Identifying New Lipid Binding Proteins to Assessing their Binding Properties.- Using Genetically Encoded Fluorescent Reporters to Image Lipid Signaling in Living Plants.- Imaging Changes in Cytoplasmic Calcium using the Yellow Cameleon 3.6 Biosensor and Confocal Microscopy.

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