Overview

This thorough volume presents a wide range of existing methods, from the very popular to the more exotic, in the area of plant genotyping. Many methods of plant genotyping were initially developed for medical research, but all genotyping methods, if they are to be successful, should be suitable for application across the full range of studies within plant biology, as seen in this collection. Plant genotyping methods herein are based on a variety of assessments, including DNA microarray, with its hundreds of thousands of simultaneous reactions, or separate individual studies of DNA sequencing and fragment analysis, PCR and qPCR, allele-specific molecular probes and primers, digestion with restriction endonucleases, microscopy and many others. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, andtips on troubleshooting and avoiding known pitfalls.  Authoritative and practical, Plant Genotyping: Methods and Protocols offers researchers the opportunity to update their knowledge and choose the most suitable method of plant genotyping for their chosen application.

Full Product Details

Author:   Yuri Shavrukov
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2023
Volume:   2638
Weight:   1.107kg
ISBN:  

9781071630235

ISBN 10:   1071630237
Pages:   469
Publication Date:   14 February 2023
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

1. Genotyping by Sequencing (GBS) for Genome-Wide SNP Identification in Plants             Wirulda Pootakham   2. Genotyping by Multiplexed Sequencing (GMS) Using SNP Markers             Travis M. Ruff, Karol Marlowe, Marcus A. Hooker, Yan Liu, and Deven R. See   3. Computational Protocol for DNA Methylation Profiling in Plants Using Restriction Enzyme-Based Genome Reduction             Wendell Jacinto Pereira, Marília de Castro Rodrigues Pappas, and Georgios Joannis Pappas Jr.   4. Double Digest Restriction-Site Associated DNA Sequencing (ddRADseq) Technology             Natalia Cristina Aguirre, Carla Valeria Filippi, Pablo Alfredo Vera, Andrea Fabiana Puebla, Giusi Zaina, Verónica Viviana Lia, Susana Noemí Marcucci Poltri, and Norma Beatriz Paniego   5. Whole Genome Wide SSR Markers Identification Based on ddRAD-Seq Data             Gitanjali Tandon, Sarika Jaiswal, Mir Asif Iquebal, Anil Rai, and Dinesh Kumar   6. High-Throughput Association Mapping in Brassica napus L.: Methods and Applications             Rafaqat Ali Gill, Md Mostofa Uddin Helal, Minqiang Tang, Ming Hu, Chaobo Tong, and Shengyi Liu   7. Polyploid SNP Genotyping Using the MassARRAY System             Aline da Costa Lima Moraes, Danilo Augusto Sforça, Melina Cristina Mancini, Bianca Baccili Zanotto Vigna, and Anete Pereira de Souza   8. qPCR Genotyping of Polyploid Species             Haiyan Wang, Jiangbo Dang, Qigao Guo, and Guolu Liang   9. Genome-Wide Association Studies (GWAS)             Jianbo He and Junyi Gai   10. Transcriptomic Approach for Global Distribution of SNP/Indel and Plant Genotyping             Claudia Muñoz-Espinoza, Marco Meneses, and Patricio Hinrichsen   11. Specific-Locus Amplified Fragment Sequencing (SLAF-Seq)             Yang Zhou and Huitang Pan   12. Modifications of Kompetitive Allele-Specific PCR (KASP) Genotyping for Detection of Rare Alleles             Anthony Brusa, Eric Patterson, and Margaret Fleming   13. Amplifluor-Based SNP Genotyping             Manmode Darpan Mohanrao, Senapathy Senthilvel, Yarabapani Rushwanth Reddy, Chippa Anil Kumar, and Palchamy Kadirvel   14. SNP Genotyping with Amplifluor-Like Method             Gulmira Khassanova, Sholpan Khalbayeva, Dauren Serikbay, Shynar Mazkirat, Kulpash Bulatova, Maral Utebayev, and Yuri Shavrukov   15. Semi-Thermal Asymmetric Reverse PCR (STARP) Genotyping             Awais Rasheed   16. Modified Allele-Specific qPCR (ASQ) Genotyping             Aigul Amangeldiyeva, Akmaral Baidyussen, Marzhan Kuzbakova, Raushan Yerzhebayeva, Satyvaldy Jatayev, and Yuri Shavrukov   17. Allele-Specific Mutation Genotyping with Mismatches in Primer Design             Yutaro Saito, Fumito Tada, Tadashi Takashina, and Hidetoshi Ikegami   18. PCR Allele Competitive Extension (PACE)             Daniel von Maydell   19. Molecular Beacon Probe (MBP)-Based Real Time PCR             Gopal Kumar Prajapati, Ashutosh Kumar, Aakanksha Wany, and Dev Mani Pandey   20. Molecular Beacons – Loop-Mediated Amplification (MB-LAMP)             Patrick Hardinge   21. TaqMan Probes for Plant Species Identification and Quantification in Food and Feed Traceability             Maria Doroteia Campos, Catarina Campos, and Hélia Cardoso   22. Tetra-Primer Amplification Refractory Mutation System (T-ARMS)             Arnab Mukherjee and Tirthartha Chattopadhyay   23. Penta-Primer Amplification Refractory Mutation System (PARMS) with Direct PCR-Based SNP Marker-Assisted Selection (D-MAS)             Chao Tan and Yanyu Yang   24. High-Resolution Melting (HRM) Genotyping             Nayoung Kim, Ji-Su Kwon, Won-Hee Kang, and Seon-In Yeom   25. Modified High-Resolution Melting (HRM) Marker Systems Increasing Discriminability Between Homozygous Alleles             Satoshi Watanabe, Yoshiyuki Yamagata, and Nobuhiro Kotoda   26. A New SNP Genotyping Technology by Target SNP-Seq             Jian Zhang, Jingjing Yang, and Changlong Wen   27. Derived Polymorphic Amplified Cleaved Sequence (dPACS) Assay             Shiv Shankhar Kaundun, Sarah-Jane Hutchings, Joe Downes, and Ken Baker   28. Tubulin-Based Polymorphism (TBP) in Plant Genotyping             Luca Braglia, Floriana Gavazzi, Silvia Gianì, Laura Morello, and Diego Breviario   29. Multiplexed ISSR Genotyping by Sequencing (MIG-Seq)             Satoshi Nanami   30. Application of SolCAP Genotyping in Potato (Solanum tuberosum l.) Association Mapping             Muhammad Farhan Yousaf, Muhammad Abu Bakar Zia, and Muhammad Naeem   31. Fluorescence In Situ Hybridization (FISH) for Genotyping of Triticeae Tribe Species and Hybrids             Irina Adonina   32. Innovative Advances in Plant Genotyping             William J.W. Thomas, Yueqi Zhang, Junrey C. Amas, Aldrin Y. Cantila, Jaco D. Zandberg, Samantha L. Harvie, and Jacqueline Batley

Reviews

Author Information

Tab Content 6

Author Website:  

Customer Reviews

Recent Reviews

No review item found!

Add your own review!

Countries Available

All regions