Overview

This new edition explores innovative approaches and keystone methodologies reflecting the recent advances in the field of plant cell division that have enabled us to study this fascinating process in a quantitative manner, at high resolution both in space and time using cell biology, biochemistry, and molecular biology. After a review of the methods used to visualize the actin cytoskeleton during plant cell division, the book focuses first on methodology to address mitosis progression as a part of the cell cycle. It continues with sections on manipulation of cell division, quantification of cell division patterning, as well as imaging and quantifying plant cytokinesis. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and up-to-date, Plant Cell Division: Methods and Protocols, Second Edition serves as an ideal guide for researchers attempting to visualize, quantify, and modify cell division during cell cycle progression.

Full Product Details

Author:   Marie-Cécile Caillaud
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   2nd ed. 2022
Volume:   2382
Weight:   0.537kg
ISBN:  

9781071617465

ISBN 10:   107161746
Pages:   267
Publication Date:   29 October 2022
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

1. Methods to Visualize the Actin Cytoskeleton during Plant Cell Division             Marie-Cécile Caillaud   Part I: Mitosis and Cell Cycle Progression   2. Immunolabeling of Nuclei/Chromosomes in Arabidopsis thaliana             Ulkar Ahmadli, Michael Sandmann, Joerg Fuchs, and Inna Lermontova   3. A Protocol for Genome-Wide Analysis of DNA Replication Timing in Intact Root Tips             Leigh Mickelson-Young, Emily E. Wear, Jawon Song, Gregory J. Zynda, Linda Hanley-Bowdoin, and William F. Thompson   4. Assaying Cyclin-Dependent Kinase Activity in Synchronized Algal Cultures             Kateřina Bišová   5. Analysis of Commitment Point Attainment in Algae Dividing by Multiple Fission             Veronika Kselíková, Vilém Zachleder, and Kateřina Bišová   Part II: Manipulation of Cell Division   6. Automated Time-Lapse Imaging and Manipulation of Cell Divisions in Arabidopsis Roots by Vertical Stage Confocal Microscopy             Lukas Hoermayer, Jiří Friml, and Matouš Glanc   7. Transient Overexpression of E2Fb to Induce Cell Divisions in Nicotiana benthamiana Pavement Cells             Tamara Jimenez-Gόngora, Huang Tan, and Rosa Lozano-Durán   Part III: Quantification of Cell Division Patterning   8. Kinematic Growth Analysis of Rice (Oryza sativa) Leaf             Jingjing Fang and Xueyong Li   9. Studying Cell Division Plane Positioning in Early-Stage Embryos             Katia Belcram, Jean-Christophe Palauqui, and Martine Pastuglia   10. Means to Quantify Vascular Cell File Numbers in Different Tissues             Helena E. Arents, Gugan Eswaran, Matouš Glanc, Ari Pekka Mähönen, and Bert De Rybel   11. Confocal Analysis of Arabidopsis Root Cell Divisions in 3D: A Focus on the Endodermis             Roya Campos and Jaimie M. Van Norman   12. Quantification of Cell Division Angles in the Arabidopsis Root             Katia Belcram, David Legland, and Martine Pastuglia   Part IV: Imaging and Quantifying Plant Cytokinesis   13. A Quantitative Method for Evaluating Phragmoplast Morphology             Takema Sasaki and Yoshihisa Oda   14. Analyses of Protein Turnover at the Cell Plate by Fluorescence Recovery After Photobleaching during Cytokinesis             Alexandra Deli, Leda-Eleni Tympa, and Panagiotis N. Moschou   15. Cell Cycle Synchronization and Time-Lapse Imaging of Cytokinetic Tobacco BY-2 Cells             Keisho Maeda and Takumi Higaki   16. Ultrastructural Observation of Cytokinesis and Plasmodesmata Formation in Brown Algae             Chikako Nagasato, Rina Yonamine, and Taizo Motomura

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