Overview

This collection explores state-of-the-art methods and protocols for research on photodynamic therapy (PDT) and its use in a wide range of medical applications, from antiviral to anticancer. Beginning with an extensive section on in vitro and in vivo models, the volume continues with chapters on oxygen-independent photosensitizers, next-generation photosensitization strategies, contemporary insights into the immunomodulatory effects of PDT, antimicrobial effects of PDT, as well as a variety of general biochemical and molecular biological techniques. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of detailed implementation advice that ensures successful results in the lab.  Thorough and authoritative, Photodynamic Therapy: Methods and Protocols serves as an ideal source of inspiration for both new and established PDT scientists and a guide for designing innovative research programs in this continuously advancing and multidisciplinary field.

Full Product Details

Author:   Mans Broekgaarden ,  Hong Zhang ,  Mladen Korbelik ,  Michael R. Hamblin
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2022
Volume:   2451
Weight:   1.622kg
ISBN:  

9781071620984

ISBN 10:   1071620983
Pages:   752
Publication Date:   04 May 2022
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Part I: In Vitro and In Vivo Models to Study Photodynamic Therapy   1. Application of Monolayer Cell Cultures for Investigating Basic Mechanisms of Photodynamic Therapy             Mans Broekgaarden   2. The Negative Impact of Cancer Cell Nitric Oxide on Photodynamic Therapy             Jonathan M. Fahey and Albert W. Girotti   3. Microtumor Models as a Preclinical Investigational Platform for Photodynamic Therapy             Mans Broekgaarden and Jean-Luc Coll   4. A Microfluidic Model to Evaluate Photodynamic Therapy Treatment Response in 3D Tumors             Shubhankar Nath, Michael Pigula, Tayyaba Hasan, and Imran Rizvi   5. Analysis of Treatment Effects on Structurally Complex Microtumor Cultures Using a Comprehensive Image Analysis Procedure             Anne-Laure Bulin, Mans Broekgaarden, and Tayyaba Hasan   6. High-Throughput Examination of Therapy-Induced Alterations in Redox Metabolism in Spheroid and Microtumor Models             Mans Broekgaarden, Anne-Laure Bulin, and Tayyaba Hasan   7. Spatiotemporal Tracking of Different Cell Populations in Cancer Organoid Models for Investigations on Photodynamic Therapy             Anne-Laure Bulin and Tayyaba Hasan   8. Generating Large Numbers of Pancreatic Microtumors on Alginate-Gelatin Hydrogels for Quantitative Imaging of Tumor Growth and Photodynamic Therapy Optimization             Nazareth Milagros Carigga Gutierrez, Tristan Le Clainche, Jean-Luc Coll, Lucie Sancey, and Mans Broekgaarden   9. The Chicken Embryo Chorioallantoic Membrane as an In Vivo Model for Photodynamic Therapy             Jaroslava Joniová and Georges Wagnières   10. Subcutaneous Xenograft Models for Studying PDT In Vivo             Girgis Obaid and Tayyaba Hasan   11. In Vivo Models for Studying Interstitial Photodynamic Therapy of Locally Advanced Cancer             Gal Shafirstein, Emily Oakley, Sasheen Hamilton, Michael Habitzruther, Sarah Chamberlain, Sandra Sexton, Leslie Curtin, and David A. Bellnier   12. Orthotopic Models of Pancreatic Cancer to Study PDT             Girgis Obaid, Zhiming Mai, and Tayyaba Hasan   13. An Orthotopic Murine Model of Peritoneal Carcinomatosis of Ovarian Origin for Intraoperative PDT             Thierry Michy, Claire Bernard, Jean Luc Coll, and Véronique Josserand   14. Photodynamic Treatments for Disseminated Cancer Metastases Using Fiber-Optic Technologies             Eric M. Kercher and Bryan Q. Spring   15. Stereotaxic Implantation of F98 Cells in Fischer Rats: A Syngeneic Model to Investigate Photodynamic Therapy Response in Glioma             Anne-Laure Bulin, Jean-François Adam, and Hélène Elleaume   Part II: Inorganic Photosenstizers and Light-Activatable Anti-Cancer Prodrugs   16. New Generation of Photosensitizers Based on Inorganic Nanomaterials             Xiaomin Liu and Hong Zhang   17. Cytotoxicity of Metal-Based Photoactivated Chemotherapy (PACT) Compounds             Vadde Ramu, Austin B. Auyeung, and Sylvestre Bonnet   Part III: Third and Fourth-Generation Photosensitizers   18. Photosensitized Oxidation of Intracellular Targets: Understanding the Mechanisms to Improve the Efficiency of Photodynamic Therapy             Thiago Teixeira Tasso and Maurício da Silva Baptista   19. Inhibition of the HIF-1 Survival Pathway as a Strategy to Augment Photodynamic Therapy Efficacy             Mark J. de Keijzer, Daniel J. de Klerk, Lianne R. de Haan, Robert T. van Kooten, Leonardo P. Franchi, Lionel M. Dias, Tony G. Kleijn, Diederick J. van Doorn, and Michal Heger on behalf of the International Photodynamic Therapy Study Group   20. Strategies for Improving Photodynamic Therapy through Pharmacological Modulation of the Immediate Early Stress Response             Daniel J. de Klerk, Mark J. de Keijzer, Lionel M. Dias, Jordi Heemskerk, Lianne R. de Haan, Tony G. Kleijn, Leonardo P. Franchi, and Michal Heger on behalf of the International Photodynamic Therapy Study Group   21. Nanobody-Targeted Photodynamic Therapy: Nanobody Production and Purification             Vida Mashayekhi, Erik Schooten, Paul M.P. van Bergen en Henegouwen, Marta M. Kijanka, and Sabrina Oliveira   22. Conjugation of IRDye Photosensitizers or Fluorophores to Nanobodies             Vida Mashayekhi and Sabrina Oliveira   23. In Vitro Assessment of Binding Affinity, Selectivity, Uptake, Intracellular Degradation, and Toxicity of Nanobody-Photosensitizer Conjugates             Irati Beltrán Hernández, Timo W.M. De Groof, Raimond Heukers, and Sabrina Oliveira   24. Assessment of the In Vivo Response to Nanobody-Targeted PDT through Intravital Microscopy             Henriette S. de Bruijn, Ann L.B. Seynhaeve, Timo L.M. ten Hagen, Sabrina Oliveira, and Dominic J. Robinson   25. Orthotopic Breast Cancer Model to Investigate the Therapeutic Efficacy of Nanobody-Targeted Photodynamic Therapy             Marion M. Deken, Shadhvi S. Bhairosingh, Alexander L. Vahrmeijer, and Sabrina Oliveira   26. Investigation of the Therapeutic Potential of Nanobody-Targeted Photodynamic Therapy in an Orthotopic Head and Neck Cancer Model             Pieter B.A.A. van Driel, Stijn Keereweer, Clemens W.G.M. Lowik, and Sabrina Oliveira   Part IV: Photodynamic Therapy-Induced Immune Signaling   27. Evaluation of the Antitumor Immune Response Following Photofrin-Based PDT in Combination with the Epigenetic Agent 5-Aza-2’-Deoxycytidine             Malgorzata Wachowska, Angelika Muchowicz, and Jakub Golab   28. Controlling Immunoregulatory Cell Activity for Effective Photodynamic Therapy of Cancer             Mladen Korbelik, Zdzislaw M. Szulc, Alicja Bielawska, and Duska Separovic   29. Measuring the Anti-Tumor T-Cell Response in the Context of Photodynamic Therapy             Jan Willem Kleinovink and Ferry Ossendorp   30. Combination of Photodynamic Therapy and Immune Checkpoint Blockade             Jan Willem Kleinovink and Ferry Ossendorp   31. Combination of Photodynamic Therapy and Therapeutic Vaccination             Jan Willem Kleinovink and Ferry Ossendorp   Part V: Antimicrobial Photodynamic Therapy   32. In Vitro Potentiation of Antimicrobial Photodynamic Inactivation by Addition of Potassium Iodide             Nasim Kashef and Michael R. Hamblin   33. In Vivo Potentiation of Antimicrobial Photodynamic Therapy in a Mouse Model of Fungal Infection by Addition of Potassium Iodide             Nasim Kashef and Michael R. Hamblin   34. Bioluminescent Models to Evaluate the Efficiency of Light-Based Antibacterial Approaches             Ana T.P.C. Gomes, Maria A.F. Faustino, Maria G.P.M.S. Neves, and Adelaide Almeida   35. Photochemical Internalization as a New Strategy to Enhance Efficacy of Antimicrobial Agents against Intracellular Infections             Xiaolin Zhang, Leonie de Boer, and Sebastian. A. J. Zaat   36. Determination of the Efficiency of Photodynamic Decontamination of Food             Michael Glueck and Kristjan Plaetzer   Part VI: Molecular Techniques and Tools in Photodynamic Therapy Research   37. Super-Resolution Imaging of Intracellular Lipid Nanocarriers to Study Drug Delivery in Photodynamic Therapy             Enzo M. Scutigliani, Jakub A. Kochan, Emilie C.B. Desclos, Art Jonker, Michal Heger, and Przemek M. Krawczyk   38. Detection of Paraptosis after Photodynamic Therapy             David Kessel   39. Optimal Use of 2´,7´-Dichlorofluorescein Diacetate in Cultured Hepatocytes             Megan J. Reiniers, Lianne R. de Haan, Laurens F. Reeskamp, Mans Broekgaarden, Ruurdtje Hoekstra, Rowan F. van Golen, and Michal Heger

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