Overview

In this the first of two volumes, attention is focused on procedures used in the isolation of polypeptide hormones and their indentification and structural analysis; the development of sensitive bioassay and immunoassay techniques; methods in current use for tissue localization of peptide hormones and their encoding MRNAs; and techniques for characterization of peptide hormone receptors. Wherever possible, the general principles and strategies on which the methods are based are given so that the techniques will have a wide application to biologically active peptides involved in neurotransmission, growth regulations, differentiation, and vasoregulation. This and the second volume ""Peptide Hormone Action"" are also published together as a two-volume set.

Full Product Details

Author:   J. C. Hutton ,  K. Siddle
Publisher:   Oxford University Press
Imprint:   Oxford University Press
Dimensions:   Width: 23.40cm , Height: 2.50cm , Length: 15.60cm
Weight:   0.787kg
ISBN:  

9780199630684

ISBN 10:   0199630682
Pages:   362
Publication Date:   01 November 1990
Audience:   College/higher education ,  Professional and scholarly ,  Postgraduate, Research & Scholarly ,  Professional & Vocational
Format:   Spiral bound
Publisher's Status:   Active
Availability:   To order  
Stock availability from the supplier is unknown. We will order it for you and ship this item to you once it is received by us.

Table of Contents

"Peptide hormone purification, C.McLean; electrophoretic and immunoblotting methods, P.Jackson; radioimmunoassay, R.Edwards; immunometric assays, K.Siddle; the ""In Vitro"" bioassay of peptide hormones, W.R.Robertson and S.P.Bidey; ""In Situ"" hybridization for the localization and quantification of peptide mRNAs, P.C.Emson et al; localization of peptide hormones by light and electron microscopy, A.Clark and D.Pow; methods for the measurement of insulin secretion, W.J.Malaisse et al; cell biology of secretion, K.W.Brocklehurst and H.Pollard; cell permeabilization, P.E.R.Tatham and B.Gomperts; the molecular biology of polypeptide hormone expression and biosynthesis, D.S.W.Boam et al; regulation of the biosynthesis and processing of polypeptide hormones J.C.Hutton et al."

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