Overview
Proteins interacting with diverse ligands - proteins, peptides or DNA - are the basic principles underlying many biological processes, such as antigen-antibody binding, signal transduction or receptor binding.The technique of oligopeptide synthesis on a cellulose membrane and the subsequent binding assays allow the investigation of protein interactions. A particular advantage of these peptide arrays (SPOT - technology) is the high number of oligopeptide probes that can be tested in parallel. Detailed protocols for peptide synthesis, and the analysis of protein-protein, protein-DNA interactions as well as epitope mapping are presented in this manual. It is ideally suited not only for basic research laboratories but also for diagnostic and therapeutic applications since many diseases are related to dysfunctions in protein recognition and binding.
Full Product Details
Author: Joachim Koch ,
Michael Mahler
Publisher: Springer-Verlag Berlin and Heidelberg GmbH & Co. KG
Imprint: Springer-Verlag Berlin and Heidelberg GmbH & Co. K
Edition: Softcover reprint of 1st ed. 2002
Dimensions:
Width: 15.50cm
, Height: 0.90cm
, Length: 23.50cm
Weight: 0.454kg
ISBN: 9783642076398
ISBN 10: 3642076394
Pages: 173
Publication Date: 21 October 2010
Audience:
Professional and scholarly
,
Professional & Vocational
Format: Paperback
Publisher's Status: Active
Availability: In Print
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Reviews
From the reviews: In this book the authors provide a detailed overview of the technology, including insights into basic chemistry and established applications. All protocols have been written by experienced researchers in SPOT technology and have therefore been optimized for practical use. ... It is ideally suited not only for basic research laboratories but also for diagnostic and therapeutic applications since many diseases are related to dysfunctions in protein recognition and binding. (New Drugs, Issue 7, October, 2002) Detailed protocols for peptide synthesis and the analysis of protein-protein, protein-DNA interactions as well as epitope mapping are presented in this manual. It is ideally suited not only for basic research laboratories but potentially also for diagnostic and therapeutic applications ... . This book provides a detailed overview of the technology, including insights into the basic chemistry and established applications. ... The book is suitable for researchers in basic research and in therapeutic applications as well as for advanced students. (Biotech International, Vol. 15 (2), 2003)
From the reviews: In this book the authors provide a detailed overview of the technology, including insights into basic chemistry and established applications. All protocols have been written by experienced researchers in SPOT technology and have therefore been optimized for practical use. ! It is ideally suited not only for basic research laboratories but also for diagnostic and therapeutic applications since many diseases are related to dysfunctions in protein recognition and binding. (New Drugs, Issue 7, October, 2002) Detailed protocols for peptide synthesis and the analysis of protein-protein, protein-DNA interactions as well as epitope mapping are presented in this manual. It is ideally suited not only for basic research laboratories but potentially also for diagnostic and therapeutic applications ! . This book provides a detailed overview of the technology, including insights into the basic chemistry and established applications. ... The book is suitable for researchers in basic research and in therapeutic applications as well as for advanced students. (Biotech International, Vol. 15 (2), 2003)
