Overview
A superb, comprehensive collection of the most reliable protocols for PCR sequencing - all developed and described by eminent researchers and scientists who use them successfully every day. These protocols deal practically with those problems accompanying the rapid reassociation of amplified complementary strands by showing how sequencing reactions may be effectively modified, as well as complex methods of primer manipulation that enable the affinity purification, or its transcript production, of homogeneous single strands. They also provide all the necessary details of PCR purification, primer labeling, the preparation of sequencing gels, as well as of conventional methods for cloning PCR products into vectors and their subsequent sequencing.
Full Product Details
Author: Ralph Rapley
Publisher: Humana Press Inc.
Imprint: Humana Press Inc.
Edition: 1996 ed.
Volume: 65
Dimensions:
Width: 15.20cm
, Height: 1.80cm
, Length: 22.90cm
Weight: 0.474kg
ISBN: 9780896033443
ISBN 10: 0896033449
Pages: 221
Publication Date: 19 August 1996
Audience:
College/higher education
,
Professional and scholarly
,
Undergraduate
,
Professional & Vocational
Format: Spiral bound
Publisher's Status: Out of Print
Availability: Out of stock
Reviews
...this is a good collection of well presented PCR protocols that should find its way into many laboratories...if you are planning on performing a large amount of PCR sequencing, or have been having problems sequencing PCR products, this book has a procedure or advice that will help. -FEBS Letters The book is a must for investigators in molecular biology and involved with sequencing and setting up PCR protocols...I highly recommend this most comprehensive volume for all molecular biologists. - SMG Quarterly
...this is a good collection of well presented PCR protocols that should find its way into many laboratories...if you are planning on performing a large amount of PCR sequencing, or have been having problems sequencing PCR products, this book has a procedure or advice that will help. -FEBS Letters The book is a must for investigators in molecular biology and involved with sequencing and setting up PCR protocols....I highly recommend this most comprehensive volume for all molecular biologists. - SMG Quarterly
...this is a good collection of well presented PCR protocols that should find its way into many laboratories...if you are planning on performing a large amount of PCR sequencing, or have been having problems sequencing PCR products, this book has a procedure or advice that will help. - FEBS Letters The book is a must for investigators in molecular biology and involved with sequencing and setting up PCR protocols. I highly recommend this most comprehensive volume for all molecular biologists. - SMG Quarterly
