Overview

This third edition provides new and updated chapters on design PCR primers for successful DNA amplification. Chapters are divided into seven parts, including primer design strategies for quantitative PCR, genotyping, multiplex PCR, in silico PCR primer design, and primer design to identify plant and animal viruses. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Authoritative and easily accessible, PCR Primer Design, Third Edition aims to be useful for various fields of molecular biology, including biotechnology, molecular genetics, and recombinant DNA technology.

Full Product Details

Author:   Chhandak Basu
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   3rd ed. 2022
Volume:   2392
Weight:   0.559kg
ISBN:  

9781071618011

ISBN 10:   1071618016
Pages:   276
Publication Date:   14 November 2022
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

SECTION I. Primer design for genotyping The significance of PCR primer design in genetic diversity studies; exemplified by recent research into the genetic structure of marine species M. Delghandi, M.P. Delghandi, S. Goddard 2.              Enhancing cohort PASA efficiency from lessons assimilated by mutant genotyping in C. elegans.  Amita Pandey, Binu Bhat, Madan L. Aggarwal,  Girdhar K. Pandey  3.              Design of oligonucleotides for allele-specific amplification based on PCR and isothermal techniques Luis Antonio Tortajada-Genaro 4.              Detection of rubella virus by tri-primer RT-PCR assay and genotyping by fragment RT-PCR. Suji George  5.              Design of mismatch primers to identify and differentiate closely related (sub)species – application to the authentication of meat products Maria Kaltenbrunner, Rupert Hochegger, Margit Cichna-Markl 6.              Primer design for the analysis of closely related species – application of non-coding mtDNA and cpDNA sequences  Lidia Skuza  7.              Designing PCR primers for the amplification-refractory mutation system  Majid Komijani, Khashayar Shahin,  Esam Ibraheem Azhar, Mohammad Bahram   SECTION II. Primer design for genome-wide identification of specific regions  8.              Validation of circular RNAs by PCR Aniruddha Das, Debojyoti Das, and Amaresh C. Panda 9.               Primer Designing for Amplifying an AT-Rich Promoter from Arabidopsis thaliana Pinky Dhatterwal, Sandhya Mehrotra, and Rajesh Mehrotra    SECTION III. Primer design for multiplex PCR. Multiplex  10.           PLASmid TAXonomic PCR (PlasTax-PCR), a multiplex relaxase MOB typing to assort plasmids into taxonomic units Raquel Cuartas, Teresa M. Coque, Fernando de la Cruz, M. Pilar Garcillán-Barcia 11.           Multiplex PCR Design for Scalable Resequencing Darren Korbie, Matt Trau   SECTION IV. Primer design for qPCR 12.           Identification of gene copy number in the transgenic plants by quantitative polymerase chain reaction (qPCR) Poonam Kanwar, Soma Ghosh, Sibaji K. Sanyal, Girdhar K. Pandey 13.            qPrimerDB: A powerful and user-friendly database for qPCR primer design Wei Chang, Yue Niu, Mengna Yu, Tian Li, Jiana Li, Kun Lu   SECTION V. Primer design for identification of plant and animal viruses  14.           PCR primer design for the rapidly evolving SARS-CoV-2 genome Jiangyu Li, Dongsheng Zhao, Haoyang Cai and Wubin Qu 15.           Universal primers for detection of novel plant capsid-less viruses: Papaya umbra-like viruses as example Jorge H. Ramirez-Prado and Luisa A. Lopez-Ochoa   SECTION VI. Use of software for primer design 16.           A guide to using FASTPCR software for PCR, in silico PCR, and oligonucleotide analysis Ruslan Kalendar   SECTION VII. Primer design for newer PCR approaches 17.           Pyrosequencing Primer Design for Forensic Biology Applications Kelly M. Elkins  18.           Phosphate‌ ‌methylated‌ ‌oligonucleotides‌ ‌as‌ ‌a‌ ‌novel‌ ‌primer‌ ‌for‌ ‌PCR‌ ‌and‌ ‌RT-PCR‌ ‌ Yu-Hsuan‌ ‌Chang,‌ ‌Meng-Wei‌ ‌Wu,‌ ‌Yi-Ju‌ ‌Chen,‌ ‌Cao-An‌ ‌Vu,‌ ‌Ching-Ya‌ ‌Hong,‌ ‌Wen-Yih‌ ‌Chen  

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