Overview

This third edition provides new and updated chapters on design PCR primers for successful DNA amplification. Chapters are divided into seven parts, including primer design strategies for quantitative PCR, genotyping, multiplex PCR, in silico PCR primer design, and primer design to identify plant and animal viruses. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Authoritative and easily accessible, PCR Primer Design, Third Edition aims to be useful for various fields of molecular biology, including biotechnology, molecular genetics, and recombinant DNA technology.

Full Product Details

Author:   Chhandak Basu
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   3rd ed. 2022
Volume:   2392
Weight:   0.751kg
ISBN:  

9781071617984

ISBN 10:   1071617982
Pages:   276
Publication Date:   14 November 2021
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

The significance of PCR primer design in genetic diversity studies; exemplified by recent research into the genetic structure of marine species.- Enhancing cohort PASA efficiency from lessons assimilated by mutant genotyping in C. elegans.- Design of oligonucleotides for allele-specific amplification based on PCR and isothermal techniques.- Detection of rubella virus by tri-primer RT-PCR assay and genotyping by fragment RT-PCR.- Design of mismatch primers to identify and differentiate closely related (sub)species – application to the authentication of meat products.- Primer design for the analysis of closely related species – application of non-coding mtDNA and cpDNA sequences.- Designing PCR primers for the amplification-refractory mutation system.- Validation of circular RNAs by PCR.- Primer Designing for Amplifying an AT-Rich Promoter from Arabidopsis thaliana.- PLASmid TAXonomic PCR (PlasTax-PCR), a multiplex relaxase MOB typing to assort plasmids into taxonomic units.- Multiplex PCR Design for Scalable Resequencing.-  Identification of gene copy number in the transgenic plants by quantitative polymerase chain reaction (qPCR).- qPrimerDB: A powerful and user-friendly database for qPCR primer design.- PCR primer design for the rapidly evolving SARS-CoV-2 genome.- Universal primers for detection of novel plant capsid-less viruses: Papaya umbra-like viruses as example.- A guide to using FASTPCR software for PCR, in silico PCR, and oligonucleotide analysis.- Pyrosequencing Primer Design for Forensic Biology Applications.-Phosphate‌ ‌methylated‌ ‌oligonucleotides‌ ‌as‌ ‌a‌ ‌novel‌ ‌primer‌ ‌for‌ ‌PCR‌ ‌and‌ ‌RT-PCR‌. 

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