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OverviewPCR Guru: An Ultimate Benchtop Reference for Molecular Biologists is provides researchers in molecular biology with a handy reference for approaching and solving challenging problems associated with PCR setup and optimization. As a laboratory guide, it emphasizes the technical aspects of employing PCR as a tool in molecular biology laboratories. The book covers the history of PCR and the basic science underlying it. It then discusses PCR at the bench level, starting with detailed description and tips on primer design, and continuing with the standard protocols used to perform PCR. Full Product DetailsAuthor: Ayaz Najafov (Junying Yuan Lab, Department of Cell Biology, Harvard Medical School, USA) , Gerta Hoxhaj (Department of Genetics and Complex Diseases, Harvard T.H. Chan School of Public Health, USA) , Ayaz Najafov (Junying Yuan Lab, Department of Cell Biology, Harvard Medical School, USA) , Ayaz Najafov (Junying Yuan Lab, Department of Cell Biology, Harvard Medical School, USA)Publisher: Elsevier Science Publishing Co Inc Imprint: Academic Press Inc Dimensions: Width: 15.20cm , Height: 0.80cm , Length: 22.90cm Weight: 0.200kg ISBN: 9780128042311ISBN 10: 0128042311 Pages: 104 Publication Date: 09 December 2016 Audience: Professional and scholarly , Professional & Vocational Format: Paperback Publisher's Status: Active Availability: Manufactured on demand  We will order this item for you from a manufactured on demand supplier. Table of Contents1. Introduction 2. Procedure 3. Good PCR Practices 4. Troubleshooting 5. Tips 6. Special Cases Appendix A. Agarose Gel Electrophoresis Protocol Appendix B. Silver Staining of DNA Gels Appendix C. Plasmid Miniprep Protocol Appendix D. Restriction Enzyme Digestion Protocol Appendix E. Protocol for DNA Concentration Estimation by using Hoechst 33258 Appendix F. Formulas Appendix G. Primers for Common Promoters, Housekeeping Genes, Reporters, etc. Appendix H. Tags Appendix I. Thermostable DNA polymerases Appendix J. Example of a Daily PCR Log Appendix K. Common Buffers and Solutions Appendix L. Useful numbers Appendix M. Yeast colony PCRReviewsAuthor InformationAyaz was born in Baku, Azerbaijan. He obtained his BSc and MSc degrees in Molecular Biology and Genetics from Bosphorus University, Istanbul, Turkey and got a 2-year training in Biochemistry as an intern at Baylor College of Medicine, Houston, Texas, USA. Ayaz pursued his PhD in Biochemistry at MRC Protein Phosphorylation Unit at Dundee University, Scotland, UK in Prof. Dario Alessi’s lab. Ayaz is currently a postdoctoral fellow in Prof. Junying Yuan’s lab at Harvard Medical School, Cell Biology Department. Gerta was born in Fier, Albania. She obtained her BSc degrees in Molecular Biology and Genetics, as well as a second major in Chemistry from Bosphorus University, Istanbul, Turkey. Gerta pursued her PhD in Biochemistry at MRC Protein Phosphorylation Unit at Dundee University, Scotland, UK in Prof. Carol MacKintosh’s lab. Gerta is currently a postdoctoral fellow in Prof. Brendan Mannings’s lab at Harvard T.H. Chan School of Public Health, Department of Genetics and Complex Diseases. Tab Content 6Author Website:Countries AvailableAll regions |
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