Overview

This laboratory manual provides information about PCR procedures, combined with a discussion of contemporary opinions on this multifaceted method and its applications. Even experienced laboratories will welcome the clear presentation of demanding procedures such as the exact quanitification of PCR products or the in-situ PCR. Protocols that have been tried and tested provide swift support for troubleshooting all sorts of procedural problems. Indeed, many examples of the problem areas found in diagnostically oriented PCR are fully described. Furthermore, the most important lab PCR equipment as well as the pros and cons of single individual reaction components which are necessary for performing PCR reactions are discussed. The book is clearly organized to give an introduction to each technique, brief background information, step-by-step instructions for carrying out the procedure and additional comments.

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9783540554400

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an authoratative and comprehensive book on the subject...anyone contemplating introducing the method into a clinical laboratory would find this book invaluable British Journal of Biomedical Science<br> This book will provide valuable information to all those using PCR. - General Pharmacology

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