Overview

PCR in situ hybridization allows the detection of specific nucleic acid sequences and their distribution in the cell. It combines two powerful techniquesin situ hybridization (ISH), which allows cellular localization of DNA and RNA sequences in cells and tissues, and the polymerase chain reaction (PCR), which allows reproducible amplification of rare nucleic acid sequences. The combined technique and its variants greatly enhance the sensitivity of in situ hybridization and add morphological localization to the sensitivity of PCR. Such techniques have enormous potential for research and diagnosis but problems with reproducibility and reliability are often encountered. This book overcomes these problems by describing the key procedures in step-by-step detail and by providing the essential advice needed for success. Topics include: DNA in situ PCR and DNA PCR in situ hybridization (PCR ISH) for the detection of DNA targets in cells; reverse trancriptase in situ PCR (RT-PCR) and RT-PCR ISH for the detection of RNA targets; and PRINS (primed in situ synthesis) for chromosomal analysis in interphase nuclei and metaphase chromosome spreads. There are further chapters on fixation of tissues for PCR, selective ultraviolet radiation fractionation (SURF), application of in situ PCR to human tissues, applications and modifications of PCR-ISH, and automation of in situ amplification. PCR In Situ Hybridization is a unique and timely collection of well-tested protocols for the amplification of DNA and RNA in cells and tissues, drawing on the accumulated knowledge and experience of leading exponents of these techniques. For each topic covered, the authors provide detailed guidance on the key steps in the protocols, numerous hints and tips for success, and advice on trouble-shooting. PCR In Situ Hybridization will be invaluable to molecular biologists, pathologists, geneticists, and all those seeking to perform in situ analyses of nucleic acid molecules.

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9780199636327

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This is one of an impressive series of laboratory handbooks entitled The Practical Approach Series...The presentation of this material is of uniformly high quality...this book would be a valuable resource in a lab Canadian Journal of Medical Laboratory Scince ...the book gives an excellent overview of the practical application of PCRIS. Molecular Pathology, 1999

...the book gives an excellent overview of the practical application of PCRIS. Molecular Pathology, 1999 This is one of an impressive series of laboratory handbooks entitled The Practical Approach Series...The presentation of this material is of uniformly high quality...this book would be a valuable resource in a lab Canadian Journal of Medical Laboratory Scince

<br> The combination of the polymerase chain reaction (PCR) with in situ hybridization creates a powerful new technique that combines the best of both methods, enhances the sensitivity of in situ hybridization, and allows the morphological localization of the products of the PRC reaction. This volume. . .provides detailed protocols for the use of polymerase chain reaction on tissues, interphase cells, and metaphase chromosomes. 4 Stars. --Doody's Journal<br> Polymerase chain reaction (PCR) in situ hybridization (ISH) is a hybrid technique . . . The conventional use of ISH allows cellular detection and localization of DNA and RNA by hybridization with complementary nucleic acids probes and, thus, helps in understanding the disease process. PCR, on the other hand, allows the detection of minute nucleic acids sequences, but it does not permit morphological correlation . . . The combination of both PCR and ISH has greatly enhanced the morphological localization of specific nucleic acids se

The combination of the polymerase chain reaction (PCR) with in situ hybridization creates a powerful new technique that combines the best of both methods, enhances the sensitivity of in situ hybridization, and allows the morphological localization of the products of the PRC reaction. This volume. . .provides detailed protocols for the use of polymerase chain reaction on tissues, interphase cells, and metaphase chromosomes. 4 Stars. --Doody's Journal<br> Polymerase chain reaction (PCR) in situ hybridization (ISH) is a hybrid technique . . . The conventional use of ISH allows cellular detection and localization of DNA and RNA by hybridization with complementary nucleic acids probes and, thus, helps in understanding the disease process. PCR, on the other hand, allows the detection of minute nucleic acids sequences, but it does not permit morphological correlation . . . The combination of both PCR and ISH has greatly enhanced the morphological localization of specific nucleic acids sequence in cells and tissues. . . . Overall, this book covers all aspects of the technical details regarding PCR in situ hybridization and it will find wide use in those laboratories who perform or intend to perform in situ analyses of nucleic acids. The editors ought to be complimented for putting together the state of the art techniques in one book, which may serve as a useful source in this technical field for a long time. --American Journal of Human Biology<br>

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