Overview

Even if you think you know all there is to know about this technique, you should buy this book. You will be pleasantly surprised by the helpful tips you can derive from it. With applications in such diverse areas as forensic and diagnostic medicine, genetic engineering, and the food industry, the polymerase chain reaction is of great importance to biomedical research. This book will be invaluable to scientists, emphasizing the practical applications of the technique.

Full Product Details

Author:   M. J. McPherson (Department of Biochemistry) ,  P. Quirke (Department of Pathology, both at the University of Leeds) ,  G. R. Taylor (Yorkshire Regional DNA Laboratory)
Publisher:   Oxford University Press
Imprint:   Oxford University Press
Volume:   77
Dimensions:   Width: 14.70cm , Height: 1.50cm , Length: 23.30cm
Weight:   0.478kg
ISBN:  

9780199631964

ISBN 10:   0199631964
Pages:   274
Publication Date:   05 September 1991
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Out of Print
Availability:   In Print  
Limited stock is available. It will be ordered for you and shipped pending supplier's limited stock.

Table of Contents

Graham R. Taylor: Polymerase chain reaction: basic principles and automation; Adrian Ivinson, & Graham R. Taylor: Polymerase chain reaction in genetic diagnosis; David P. Jackson, Julie D. Hayden, & Philip Quirke: Extraction of nucleic acid from fresh and archival material; Roland G. Roberts, A. Jane Montandon, Peter M. Green, & David R. Bentley: Analysis of genomic sequence variation using amplification and mismatch detection; Belinda J.F. Rossiter, Markus Grompe, & C. Thomas Caskey: Detection of deletions and point mutations; Michael Litt: PCR of TG microsatellites; Susan A. Ledbetter, & David L. Nelson: Genome amplification using primers directed to interspersed repetitive sequences (IRS-PRC); Daniel H. Johnson: PCR amplification of microdissected DNA; Jonathon Silver: Inverse polymerase chain reaction; Sarah Jane Gurr, & Michael J. McPherson: PCR-directed cDNA libraries; Michael J. McPherson, Kerrie M. Jones, & Sarah Jane Gurr: PCR with highly redundant primers; Tim Clackson, Detlef Gussow, & Peter T. Jones: General application of PCR to gene cloning and manipulation; Margaret J. Dallman, & Andrew C.G. Porter: Semi-quantitative PCR for the analysis of gene expression; Kirstin A. Eckert, & Thomas A. Kunkel: The fidelity of DNA polymerases used in the PCR; Appendix, Index

Reviews

This volume should be useful for those who have some experience in the PCR method and wish to extend the technology to more sophisticated and newer applications. --American Journal of Physiology<br> The text and the procedures in these chapters and throughout the book are clearly written and often aided by the use of graphics and photographs of key elements. . . . an excellent presentation and organization of procedureal materials related to PCR. --Journal of the American Chemical Society<br>

This volume should be useful for those who have some experience in the PCR method and wish to extend the technology to more sophisticated and newer applications. --American Journal of Physiology The text and the procedures in these chapters and throughout the book are clearly written and often aided by the use of graphics and photographs of key elements. . . . an excellent presentation and organization of procedureal materials related to PCR. --Journal of the American Chemical Society

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