Overview

This volume delivers a compendium of detailed protocols to the research community in order to aid in the investigation and discovery of Yersinia virulence mechanisms using important in vivo and in vitro infection models, which have led to major advances in the field and in our understanding of Yersinia pathogen-host interactions. Beginning with a section on mouse models, the book continues with chapters covering the monitoring of bacteria during infection, invertebrate models, as well as Yersinia interaction with immune cells and immune signaling. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.  Comprehensive and authoritative, Pathogenic Yersinia: Methods and Protocols providesa single source for researchers seeking to better understand these pathogens and the diseases they produce in humans.

Full Product Details

Author:   Viveka Vadyvaloo ,  Matthew B. Lawrenz
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   1st ed. 2019
Volume:   2010
Weight:   0.522kg
ISBN:  

9781493995431

ISBN 10:   149399543
Pages:   259
Publication Date:   14 August 2020
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

1.    Media culturing methods for selection and biochemical identification of specific Yersinia phenotypes 2.    Plague Vertebrate Infection models:  Intradermal inoculation 3.    Plague Vertebrate Infection models Intranasal inoculation 4.    Plague Vertebrate Infection models Aerosol inoculation 5.    Plague Vertebrate Infection models Septicemic inoculation 6.    Plague Vertebrate Infection models Flea-borne transmission 7.    Plague Invertebrate Infection model: Caenorhabditis elegans 8.    Plague Invertebrate Infection models: Flea Model 9.    Plague Invertebrate Infection models: Drosophila Model 10. Yersiniosis Infection models: Oral gavage 11. Immune cell infection model: Macrophage model 12. Immune cell infection model: Neutrophil model 13. Intravital microscopy of early bubonic plague events 14. Bioluminescence imaging to track bacterial colonization and dissemination 15. Mutagenesis of the Yersinia spp 16. Dual tissue RNAseq 17. Thermosensor RNA regulation 18. The Type III secretion system (T3SS): in vitro 19. The Type III secretion system (T3SS): in vitro 20. In vivo gene expression 21. Metal studies for plague 22. The Yersinia Inflammasome 23. Proteomic Bioforensic detection methods 

Reviews

The book is written for the research community, including novices. Clinicians and parasitologists with an interest in investigation and discovery of Yersinia virulence mechanisms may also find this book helpful and interesting. ... This is a wonderful resource for understanding the investigation and discovery of the virulence mechanisms of Yersinia. ... This book is quite unique in covering the methods and protocols related to Yersinia pathogen-host interactions. (Omer Iqbal, Doody's Book Reviews, November 01, 2019)

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