Overview

Pioneering bench researchers describe in detail the cutting-edge techniques they have developed for analyzing the genomes and gene products in a diverse range of protozoan and metazoan parasites. These readily reproducible techniques can be used in genomic, functional genomic, and post-genomic studies and include transfection methods and vectors for several protozoan parasites, global analysis using microarrays, gene ablation using RNA interference, gene knockout, mutagenesis, and chromosome manipulation. Some of the protocols require DNA sequence data, whereas others were developed independently of whole-genome sequence data. The protocols presented follow the successful Methods in Molecular Biology series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and the avoidance of known pitfalls.

Full Product Details

Author:   Sara E. Melville
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2004 ed.
Volume:   270
Dimensions:   Width: 15.50cm , Height: 3.10cm , Length: 23.50cm
Weight:   0.949kg
ISBN:  

9781588290625

ISBN 10:   158829062
Pages:   452
Publication Date:   17 May 2004
Audience:   College/higher education ,  Professional and scholarly ,  Postgraduate, Research & Scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

Sequencing Strategies for Parasite Genomes Daniella Bartholomeu and Najib M. El-Sayed Annotation of Parasite Genomes Matthew Berriman and Midori Harris Parasite Genome Databases and Web-Based Resources Christiane Hertz-Fowler and Neil Hall Expressed Sequence Tags: Medium-Throughput Protocols Claire Whitton, Jennifer Daub, Marian Thompson, and Mark Blaxter Expressed Sequence Tags: Analysis and Annotation John Parkinson and Mark Blaxter Positive Selection Scanning of Parasite DNA Sequences Winston A. Hide and Raphael D. Isokpehi RACE and RAGE Cloning in Parasitic Microbial Eukaryotes Bryony A. P. Williams and Robert P. Hirt Amplified (Restriction) Fragment Length Polymorphism (AFLP) Analysis Daniel K. Masiga and C. Michael R. Turner Minisatellites and MVR-PCR for the Individual Identification of Parasite Isolates Annette MacLeod Analysis of Differentially Expressed Parasite Genes and Proteins Using Transcriptomics and Proteomics Daniel C. Gare Gene Expression Studies Using Self-Fabricated Parasite cDNA Microarrays Karl F. Hoffmann and Jennifer M. Fitzpatrick DNA Content Analysis on Microarrays Upinder Singh, Preetam H. Shah, and Ryan C. MacFarlane Typing Single-Nucleotide Polymorphisms in Toxoplasma gondii by Allele-Specific Primer Extension and Microarray Detection Chunlei Su, Christian Hott, Bernard H. Brownstein, and L. David Sibley Transfection of the Human Malaria Parasite Plasmodium falciparum Brendan S. Crabb, Melanie Rug, Tim-Wolf Gilberger, Jennifer K. Thompson, Tony Triglia, Alexander G. Maier, and Alan F. Cowman A PCR-Based Method for Gene Deletion and Protein Tagging in Trypanosoma brucei George K. Arhin, Shuiyuan Shen, Elisabetta Ullu, and Christian Tschudi Analysis of Gene Function in Trypanosoma brucei Using RNA Interference Appolinaire Djikeng, Shuiyuan Shen, Christian Tschudi, and Elisabetta Ullu In Vitro Shuttle Mutagenesis Using Engineered Mariner Transposons Kelly A. Robinson, Sophie Goyard, and Stephen M. Beverley Random Mutagenesis Strategies for Construction of Large and Diverse Clone Libraries of Mutated DNA Fragments Sudsanguan Chusacultanachai and Yongyuth Yuthavong Separation, Digestion, and Cloning of Intact Parasite Chromosomes Embedded in Agarose Vanessa Leech, Michael A. Quail, and Sara E. Melville Chromosome Fragmentation in Leishmania Pascal Dubessay, Christine Blaineau, Patrick Bastien, and Michel Pagès FISH Mapping for Helminth Genomes Hirohisa Hirai and Yuriko Hirai Fiber-FISH: Fluorescence In Situ Hybridization on Stretched DNA Klaus Ersfeld Yeast Two-Hybrid Assay for Studying Protein-Protein Interactions Ahmed Osman From Genomes to Vaccines for Leishmaniasis Carmel B. Stober Index

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.,. represents an excellent resource for both students and researchers in the field. - Society for General Microbiolgy

"""...represents an excellent resource for both students and researchers in the field."" - Society for General Microbiolgy"

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