Overview

General Description of the Series: The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.

Full Product Details

Author:   John N. Abelson (California Institute of Technology, Division of Biology, Pasadena, U.S.A.) ,  Melvin I. Simon (The Salk Institute, La Jolla, CA, USA) ,  Helmut Sies (Heinrich-Heine-University Düsseldorf, Germany) ,  John N. Abelson (California Institute of Technology, Division of Biology, Pasadena, U.S.A.)
Publisher:   Elsevier Science Publishing Co Inc
Imprint:   Academic Press Inc
Volume:   v. 299
Dimensions:   Width: 15.20cm , Height: 3.50cm , Length: 22.90cm
Weight:   0.850kg
ISBN:  

9780121822002

ISBN 10:   0121822001
Pages:   506
Publication Date:   05 November 1998
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Out of Print
Availability:   In Print  
Limited stock is available. It will be ordered for you and shipped pending supplier's limited stock.

Table of Contents

"Total Antioxidant Activity: H. Alho and J. Leinonen, Total Antioxidant Activity Measured by Chemiluminescence Methods. I.F.F. Benzie and J.J. Strain, Ferric Reducing/Antioxidant Power Assay: Direct Measure of ""Total Antioxidant Activity"" of Biological Fluids, and a Modified Version (FRASC) Which Enables the Simultaneous Measurement of Total Antioxidant Power and Ascorbic Acid Concentration. Y. Noda, M. Kohno, A. Mori, and L. Packer, Automated ESR Free Radical Detector Assays for Antioxidant Activity in Natural Extracts. A. Kontush and U. Beisiegel, Measurement of Oxidizability of Blood Plasma. G. Cao and R.L. Prior, Measurement of Oxygen Radical Absorbance Capacity in Biological Samples. Vitamin C: M. Levine, Y. Wang, and S.C. Rumsey, Analysis of Ascorbic and Dehydroascorbic Acid in Biological Samples. A.M. Bode and R.C. Rose, Analysis of Water-Soluble Antioxidants by High-Performance Liquid Chromatography with Electrochemical Detection. J. Lykkesfeldt and B.N. Ames, Ascorbic Acid Recycling in Rat Hepatocytes as a Measurement of Antioxidant Capacity: Decline with Age. Polyphenols and Flavonoids: L.C. Bourne and C. Rice-Evans, Method for Detecting and Measuring Bioavailability of Phenolics and Flavonoids in Humans: Pharmacokinetics of Urinary Excretion of Dietary Ferulic Acid. T. Goto and Y. Yoshida, Simultaneous Analysis of Individual Catechins and Caffeine in Green Tea. A.L. Waterhouse, S.F. Price, and J.D. McCord, Reverse Phase HPLC Methods for Analysis of Wine Polyphenols. D.M. Goldberg and G.J. Soleas, Analysis of Antioxidant Wine Polyphenols by High-Performance Liquid Chromatography. D.M. Goldberg and G.J. Soleas, Analysis of Antioxidant Wine Polyphenols by Gas Chromatography-Mass Spectrometry. V.L. Singleton, R. Orthofer, and R.M. Lamuela-Raventós, Analysis of Total Phenols and Other Oxidation Substrates and Antioxidants by Means of Folin-Ciocalteau Reagent. V. Cheynier, J.-M. Souquet, E. LeRoux, S. Guyot, and J. Riguad, Size Separation of Condensed Tannins by Normal-Phase HPLC. R.M. Lamuela-Raventós and A. Waterhouse, Resveratrol and Piceid in Wine. E.N. Frankel, Antioxidant Activity by Headspace Gas Chromatography of Volatile Oxidation Products of w-6 and w-3 p Polyunsaturated Lipids. P.C.H. Hollman, D.P. Venema, S. Khokhar, and I.C.W. Arts, Determination of Tea Catechins by Reversed Phase HPLC. A.S. Pannala, S. Singh, and C. Rice-Evans, Flavonoids as Peroxynitrite Scavengers in Vitro. Thiols: C.K. Sen, S. Roy, S. Khanna, and L. Packer, Determination of Oxidized and Reduced Lipoic Acid Using High Performance Liquid Chromatography and Coulometric Detection. C.K. Sen, S. Roy, and L. Packer, Flow Cytometric Determination of Cellular Thiols. L.A. Ridnour, R.A. Winters, N. Ercal, and D.R. Spitz, Measurement of Glutathione, Glutathione Disulfide and Other Thiols in Mammalian Cell and Tissue Homogenates Using HPLC Separation of N-(1n-pyrenyl)maleimide Derivatives. M. Asensi, J. Sastre, F.V. Pallardo, A. Lloret, M. Lehner, J. Garcia-De-La Asuncion, and J. Viña, GSH:GSSG Ratio as an Indicator of Oxidative Stress Status and DNA Damage. J. Chaudiere, N. Aguini, and J.-C. Yadan, Nonenzymatic Colorimetric Assay of Glutathione in the Presence of Other Mercaptans. H. Baker, B. DeAngelis, E.R. Baker, and S.H. Hutner, Protozoological Method for Assaying Lipoate in Human Biologic Fluids and Tissue. O. Tirosh, Y. Katzhendler, Y. Barenholz, and R. Kohen, Antioxidant Activity of Amidothionophosphates. K. Traber and L. Packer, Quantitation of Anethole Dithiolthione Using High Pressure Liquid Chromatography with Electrochemical Detection. Vitamin E and Coenzyme Q10: D.C. Liebler, J.A. Burr, and A.J.L. Ham, Gas Chromatography-Mass Spectrometry Analysis of Vitamin E and Its Oxidation Products. J.K.G. Kramer, R.C. Fouchard, and K.M.R. Kallury, Improved Method to Determine Vitamin E Forms in Tissues and Diets by High Performance Liquid Chromatography Using a Normal-Phase Diol Column. M. Podda, C. Weber, M.G. Traber, R. Milbradt, and L. Packer, Sensitive HPLC Techniques for Simultaneous Determination of Tocopherols, Tocotrienols, Ubiquinols, and Ubiquinones in Biological Samples. B. Finckh, A. Kontush, J. Commentz, C. Hübner, M. Burdelski, and A. Kohlschütter, HPLC Analysis of Ubiquinol-10, Ubiquinone-10, Carotenoids, and Tocopherols in Neonatal Plasma--Coulometric Electrochemical Detection. C.-P. Aebischer, J. Schierle, and W. Schüep, Simultaneous Determination of Retinol, Tocopherols, Carotene Lycopene and Xanthophylls in Plasma by Means of Reversed Phase HPLC. P.K. Witting, D. Mohr, and R. Stocker, Assessment of the Prooxidant Activity of Vitamin E in Human Low Density Lipoprotein and Plasma. Carotenoids and Retinoids: N. Pellegrini, R. Re, M. Yang, and C.A. Rice-Evans, Screening of Dietary Carotenoids and Carotenoid-Rich Fruit Extracts for Antioxidant Activities Applying the ABTs Radical Cation Decolorization Assay. T. Wingerath, D. Kirsch, R. Kaufmann, W. Stahl, and H. Sies, Matrix-Assisted Laser Desorption Ionization Postsource Decay Mass Spectrometry (MALDI-PSD-MS) in Carotenoid Analysis. A. Mortensen and L.H. Skibsted, Carotenoid Photobleaching. M.A. Livrea and L. Tesoriere, Interactions Between Vitamin A and Vitamin E in Liposomes and in Biological Contexts. T.E. Gundersen and R. Blomhoff, On-Line Solid Phase Extraction and Isocratic Separation of Retinoic Acid Isomers in a Micro-bore Column Switching System. M.R. Lakshman and M.N. Rao, Purification and Characterization of Cellular Carotenoid-Binding Protein from Mammalian Liver. J.P. Landrum, R.A. Bone, L.L. Moore, and C.M. Gomez, Analysis of Zeaxanthin Stereoisomer Distribution within Individual Human Retinas. Subject Index."

Reviews

Praise for the Series ""The Methods in Enzymology series represents the gold-standard."" --NEUROSCIENCE ""Incomparably useful."" --ANALYTICAL BIOCHEMISTRY ""It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page."" --BIO/TECHNOLOGY ""The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection."" --CHEMISTRY IN INDUSTRY ""The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced."" --AMERICAN SOCIETY OF MICROBIOLOGY NEWS ""If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work."" --ENZYMOLOGIA ""A series that has established itself as a definitive reference for biochemists."" --JOURNAL OF CHROMATOGRAPHY

Praise for the Series The Methods in Enzymology series represents the gold-standard. --NEUROSCIENCE Incomparably useful. --ANALYTICAL BIOCHEMISTRY It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page. --BIO/TECHNOLOGY The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection. --CHEMISTRY IN INDUSTRY The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced. --AMERICAN SOCIETY OF MICROBIOLOGY NEWS If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work. --ENZYMOLOGIA A series that has established itself as a definitive reference for biochemists. --JOURNAL OF CHROMATOGRAPHY

Author Information

Helmut Sies, MD, PhD (hon), studied medicine at the universities of Tübingen, Munich, and Paris. He was the professor and chair of the Institute for Biochemistry and Molecular Biology I at Heinrich-Heine-University Düsseldorf, Germany, where he is now professor emeritus. He is a member of the German National Academy of Sciences Leopoldina and was the president of the North Rhine-Westphalian Academy of Sciences and Arts. He was named ‘Redox Pioneer’; was the president of the Society for Free Radical Research International (SFRRI). Helmut Sies introduced the concept of “Oxidative Stress” in 1985, and was the first to reveal hydrogen peroxide as a normal constituent of aerobic cell metabolism. His research interests comprise redox biology, oxidants, antioxidants, micronutrients.

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