Overview

This detailed volume provides a robust set of methods to understand variation between patients with ovarian cancers, in vitro models to better study different stages of the disease, and in vivo models to test therapies. Beginning with clinical perspectives to orient basic scientists, the book continues with sections exploring methods to characterize features of the tumor microenvironment (TME), techniques to isolate cells for in vitro study, and in vitro and in vivo models to study the disease. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and practical, Ovarian Cancer: Methods and Protocols serves as an ideal guide for researchers seeking to improve their study of ovarian cancer and find newtherapeutic approaches.

Full Product Details

Author:   Pamela K. Kreeger
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2022
Volume:   2424
Weight:   0.640kg
ISBN:  

9781071619582

ISBN 10:   1071619586
Pages:   318
Publication Date:   18 December 2022
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Part I: Clinical Perspectives   1. Clinical Staging of Ovarian Cancer             Andrea S. O’Shea   2. Pathologic Classification of Ovarian Cancer             Stephanie M. McGregor   Part II: Tumor Microenvironment   3. Multiparameter Flow Cytometry for Detailed Characterization of Peritoneal Immune Cells from Patients with Ovarian Cancer             Jessica Vazquez, Dagna Sheerar, Aleksandar K. Stanic, and Manish S. Patankar   4. Mass Cytometry for the Characterization of Individual Cell Types in Ovarian Solid Tumors             Veronica D. Gonzalez, Ying-Wen Huang, and Wendy J. Fantl   5. Processing and Analysis of Ascites             Hannah M. Micek, Molly J. Carroll, Lisa Barroilhet, and Pamela K. Kreeger   6. Multispectral Staining and Analysis of Extracellular Matrix             Carine M. Renner, Mike R. Visetsouk, Pamela K. Kreeger, and Kristyn S. Masters   7. Quantitative Analysis of the Extracellular Matrix by Immunoblot             Kaitlin C. Fogg   Part III: Cell Isolation   8. Multiparameter Single Cell Characterization of Ovarian Intratumor Heterogeneity             Kristin G. Beaumont, Christina Andreou, Ethan Ellis, and Robert Sebra   9. Culturing Primary Human Mesothelial Cells             Mary Mullen, Hollie Noia, and Katherine Fuh   10. Isolation of Normal and Cancer-Associated Fibroblasts             Katarzyna Zawieracz and Mark A. Eckert   11. Isolation of Primary Normal and Cancer-Associated Adipocytes from the Omentum             Abir Mukherjee   Part IV: Model Systems   12. Isolation of Fallopian Tube Epithelium and Ovaries for Assessment of Cilia Beating Frequency (CBF)             Angela Russo and Joanna E. Burdette   13. Ex Vivo Ovarian Culture to Model the Initial Metastasis in Ovarian Cancer             Matthew Dean   14. In Vivo and Ex Vivo Analysis of Omental Adhesion in Ovarian Cancer             Elizabeth I. Harper and Tyvette S. Hilliard   15. The Role of the Tumor Microenvironment in CSC Enrichment and Chemoresistance: 3D Co-Culture Methods             Michael Bregenzer, Eric Horst, Pooja Mehta, Catherine Snyder, Taylor Repetto, and Geeta Mehta   16. Establishment of In Vivo Ovarian Cancer Mouse Models Using Intraperitoneal Tumor Cell Injection             Sonam Mittal, Prachi Gupta, Pradeep Chaluvally-Raghavan, and Sunila Pradeep   17. Humanized Patient-Derived Xenograft Models of Ovarian Cancer             Sarah B. Gitto, Erin George, Sergey Medvedev, Fiona Simpkins, and Daniel J. Powell, Jr.   18. Xenograft Models of Ovarian Cancer for Therapy Evaluation             Mihaela Popa, Vibeke Fosse, Katrin Kleinmanns, Line Bjørge, and Emmet McCormack   19. Confocal Imaging of Single-Cell Signaling in Orthotopic Models of Ovarian Cancer             Dylan O. Matvey, Thomas S.C. Ng, and Miles A. Miller

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