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Author:   Jennifer Westendorf
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2008 ed.
Volume:   455
Dimensions:   Width: 15.50cm , Height: 2.00cm , Length: 23.50cm
Weight:   0.587kg
ISBN:  

9781627038478

ISBN 10:   1627038477
Pages:   354
Publication Date:   03 November 2014
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

In Vitro Models.- Isolation and Culture of Rodent Osteoprogenitor Cells.- Osteoclast Culture and Resorption Assays.- Assessing New Bone Formation in Neonatal Calvarial Organ Cultures.- Detection of Apoptosis of Bone Cells In Vitro.- In Vivo Rodent Models.- In Vivo Parathyroid Hormone Treatments and RNA Isolation and Analysis.- Assessment of Bone Formation Capacity Using In vivo Transplantation Assays: Procedure and Tissue Analysis.- Ossicle and Vossicle Implant Model Systems.- Utility of the Ovariectomized Rat as a Model for Human Osteoporosis in Drug Discovery.- Orchidectomy Models of Osteoporosis.- Methods of Gene Delivery.- Gene Delivery by Adenoviruses.- Lentivirus Delivery of shRNA Constructs into Osteoblasts.- Gene Delivery by Retroviruses.- Molecular and Genetic Techniques.- Chromatin Immunoprecipitation Assays: Application of ChIP-on-Chip for Defining Dynamic Transcriptional Mechanisms in Bone Cells.- Identification of Transcription Factor Target Genes by ChIP Display.- Application of the Laser Capture Microdissection Technique for Molecular Definition of Skeletal Cell Differentiation In Vivo.- Quantitative Trait Loci Mapping.- Imaging Techniques.- In Situ Nuclear Organization of Regulatory Machinery.- Bioluminescent Imaging in Bone.- Micro-Computed Tomography: A Method for the Non-Destructive Evaluation of the Three-Dimensional Structure of Biological Specimens.- Fourier Transform-Infrared Microspectroscopy and Microscopic Imaging.- Mechanical Testing.- Assessment of the In Vivo Adaptive Response to Mechanical Loading.- Determination of Bone's Mechanical Matrix Properties by Nanoindentation.- Fluid Flow Assays.

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