Overview

This detailed second edition provides key updates on well-established methods as well as coverage of recently emerging sophisticated techniques and methods that are essential for an in-depth and state-of-the-art understanding of skeletal development and homeostasis and the pathological mechanisms that cause skeletal degeneration. The first half of the book examines cell and molecular biological aspects of osteoarthritis and osteoporosis, while the remainder of the collection focuses on in vivo models of skeletal tissue injury, degeneration, and repair. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and up-to-date, Osteoporosis and Osteoarthritis, Second Edition aims to assist investigators concerned with research topics broadly related to osteoporosis, osteoarthritis, intervertebral disc degeneration, as well as other musculoskeletal disorders.

Full Product Details

Author:   Andre J. van Wijnen ,  Marina S. Ganshina
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   2nd ed. 2021
Volume:   2221
Weight:   0.794kg
ISBN:  

9781071609880

ISBN 10:   1071609882
Pages:   302
Publication Date:   27 September 2020
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Isolation of Murine and Human Osteocyte.- Expansion and Chondrogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stromal Cells.- A Novel Enzymatic Digestion Approach for Isolation and Culture of Rodent Bone Marrow Mesenchymal Progenitors.- Isolation of Nucleus Pulposus and Annulus Fibrosus Cells from the Intervertebral Disc.- Engineering Cartilage Tissue by Co-Culturing of Chondrocytes and Mesenchymal Stromal Cells.- Generation of Induced Pluripotent Stem Cells.- Specimen Preparation for Single Cell Sequencing Analysis of Skeletal Cells.- Mapping 5-Hydroxymethylcytosine (5hmC) Modifications in Skeletal Tissues Using High-Throughput Sequencing.- Using FRAP to Quantify Changes in Transcription Factor Dynamics after Cell Stimulation: Cell Culture, FRAP, Data Analysis, and Visualization.- Quantitative Molecular Models for Biological Processes: Modeling of Signal Transduction Networks with ANIMO.- Generation and Characterization of Mouse Models for Skeletal Disease.- Drill Hole Models to Investigate Bone Repair.- Generation and Experimental Outcomes of Closed Femoral Fracture in Mice.- Mouse Models of Osteoarthritis: Surgical Model of Post-Traumatic Osteoarthritis Induced by Destabilization of the Medial Meniscus.- Immunostaining of Skeletal Tissues.- Mapping Regional Cortical Bone Responses to Local Changes in Loading and Systemic Stimuli.- Pain and Activity Measurements.

Reviews

This book is written for investigators in the field of osteoporosis and osteoarthritis. In my opinion, in addition to the investigators in this field, the book will also be quite useful for students, researchers, and practitioners in the field of orthopedic surgery. ... This is an extremely useful book for students, investigators, orthopedic surgeons, and others who might be interested in learning more about research techniques involved in osteoporosis and osteoarthritis. ... This edition is very much needed. (Omer Iqbal, Doody's Book Reviews, February 8, 2021)

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